Correlation between gene expression and MRI STIR signals in patients with chronic low back pain and Modic changes indicates immune involvement

Flåm, Siri Tennebø; Vigeland, Magnus Dehli; Espeland, Ansgar; Kristoffersen, Per Martin; Vetti, Nils; Wigemyr, Monica; Bråten, Lars Christian Haugli; Gjefsen, Elisabeth; Schistad, Elina Iordanova; Haugen, Anne Julsrud; Froholdt, Anne; Skouen, Jan Sture; Zwart, John‐Anker; Storheim, Kjersti; Pedersen, Linda M.; Lie, Benedicte A.; Anke, Audny; Winsvold, Bendik Slagsvold; Lurud, Britt Elin; Hellum, Christian; Andersen, Erling D.; Granvigen, Fredrik; Marchand, Gunn Hege; Kjos, Guro; Andersen, Hege; Presberg, Hilde; Østhus, Ida Beate Øyen; Brox, Jens Ivar; Aßmus, Jörg; Gammelsrud, Karianne Wiger; Huneide, Knut Morten; Grøvle, Lars; Rolfsen, Mads Peder; Wilhelmsen, Maja; Grotle, Margreth; Thorsø, Marianne; Lutro, Olav; Nygaard, Øystein P.; Randen, Sigrun; Claussen, Siv Krüger; Fors, Terese; Kadar, Thomas; Holmgard, Thor Einar; Sørensen, Veronica; Rao, Vidar

doi:10.1038/s41598-021-04189-5

Cited by 6 publications

(4 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Modic changes cause the degeneration of the lumbar spine on imaging and are probably involved in the body’s immune response. 30 , 31 Pradip et al found that Modic changes were chronic subclinical infection foci rather than degeneration markers alone. 32 Ohtori et al reported that endplate abnormality is associated with TNF-induced axonal development and inflammation.…”

Section: Discussionmentioning

confidence: 99%

Using Machine Learning to Predict Surgical Site Infection After Lumbar Spine Surgery

Chen¹,

Liu²,

Zhang³

et al. 2023

IDR

View full text Add to dashboard Cite

The objective of this study was to utilize machine learning techniques to analyze perioperative factors and identify blood glucose levels that can predict the occurrence of surgical site infection following posterior lumbar spinal surgery. Methods: A total of 4019 patients receiving lumbar internal fixation surgery from an institute were enrolled between June 2012 and February 2021. First, the filtered data were randomized into the test and verification groups. Second, in the test group, specific variables were screened using logistic regression analysis, Lasso regression analysis, support vector machine, and random forest. Specific variables obtained using the four methods were intersected, and a dynamic model was constructed. ROC and calibration curves were constructed to assess model performance. Finally, internal model performance was verified in the verification group using ROC and calibration curves. Results: The data from 4019 patients were collected. In total, 1327 eligible cases were selected. By combining logistic regression analysis with three machine learning algorithms, this study identified four predictors associated with SSI, namely Modic changes, sebum thickness, hemoglobin, and glucose. Using this information, a prediction model was developed and visually represented. Then, we constructed ROC and calibration curves using the test group; the area under the ROC curve was 0.988. Further, calibration curve analysis revealed favorable consistency of nomogram-predicted values compared with real measurements. The C-index of our model was 0.986 (95% CI 0.981-0.994). Finally, we used the validation group to validate the model internally; the AUC was 0.987. Calibration curve analysis revealed favorable consistency of nomogram-predicted values compared with real measurements. The C-index was 0.982 (95% CI 0.974-0.999). Conclusion: Logistic regression analysis and machine learning were employed to select four risk factors: Modic changes, sebum thickness, hemoglobin, and glucose. Then, a dynamic prediction model was constructed to help clinicians simplify the monitoring and prevention of SSI.

show abstract

Section: Discussionmentioning

confidence: 99%

Using Machine Learning to Predict Surgical Site Infection After Lumbar Spine Surgery

Chen¹,

Liu²,

Zhang³

et al. 2023

IDR

View full text Add to dashboard Cite

show abstract

“…We do recognise that having published their review protocol in Prospero they had limited freedom to do so. Unfortunately, Herlin et al is often being inappropriately cited as demonstrating no association between LBP and MC without acknowledging its limitations [30][31][32][33][34][35][36][37][38], but others do cite it in a more appropriate nuanced way [23,[39][40][41][42][43][44][45][46][47][48][49].…”

Section: Discussionmentioning

confidence: 99%

“…Papers citing Herlin et al were identified using Pub-Med [23,[30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49]. The literature was searched through PubMed with the keywords "MRI (back pain) association Modic" restricted to the last 5 years and identified 135 articles.…”

Section: Methodsmentioning

confidence: 99%

Modic changes as seen on MRI are associated with nonspecific chronic lower back pain and disability

Czaplewski,

Rimmer,

McHale

et al. 2023

J Orthop Surg Res

View full text Add to dashboard Cite

Background Estimating the contribution of endplate oedema known as Modic changes to lower back pain (LBP) has been the subject of multiple observational studies and reviews, some of which conclude that the evidence for an association of Modic change with LBP is uncertain while others demonstrate a clear link. The clinical trials demonstrating the benefit of basivertebral nerve ablation, a therapeutic intervention, in a tightly defined homogenous patient group with chronic LBP and Modic changes type 1 or type 2, provides further evidence for the contribution of Modic changes to LBP and shows that in these subjects, nerve ablation substantially reduces pain and disability. These interventional studies provide direct evidence that Modic changes can be associated with lower back pain and disability. This review set out to explore why the literature to date has been conflicting. Methods A narrative, forensic, non-systematic literature review of selected articles to investigate why the published literature investigating the association between Modic imaging changes and chronic low back pain is inconsistent. Results This review found that previous systematic reviews and meta-analyses included both heterogeneous study designs and diverse patient syndromes resulting in an inconsistent association between Modic changes and nonspecific chronic lower back pain. Re-analysis of literature data focussing on more homogenous patient populations provides clearer evidence that Modic changes are associated with nonspecific chronic lower back pain and that type 1 Modic changes are more painful than type 2. Conclusions Studies using tightly defined homogenous patient groups may provide the best test for association between MRI-findings and pain and disability. Clinical benefit of basivertebral nerve ablation observed in randomised controlled trials further supports the association between type 1 and type 2 Modic changes with pain and disability.

show abstract

“…The quality of the sequencing was assessed using FastQC and Qualimap [35,36]. Preprocessing and quality control of the RNA sequencing data are described in detail elsewhere [37]. Briefly, 97.4% of the reads were successfully mapped to the human genome (GRCh38.p10) using HISAT2 v2.1.0 [38], and 45.3% of these were assigned to genes with feature counts from Subread v1.6.3 [39], using gene coordinates from Ensembl 88 [40].…”

Section: Generation Preprocessing and Quality Control Of Datamentioning

confidence: 99%

Long-Term Use of Amoxicillin Is Associated with Changes in Gene Expression and DNA Methylation in Patients with Low Back Pain and Modic Changes

et al. 2023

Self Cite

View full text Add to dashboard Cite

Long-term antibiotics are prescribed for a variety of medical conditions, recently including low back pain with Modic changes. The molecular impact of such treatment is unknown. We conducted longitudinal transcriptome and epigenome analyses in patients (n = 100) receiving amoxicillin treatment or placebo for 100 days in the Antibiotics in Modic Changes (AIM) study. Gene expression and DNA methylation were investigated at a genome-wide level at screening, after 100 days of treatment, and at one-year follow-up. We identified intra-individual longitudinal changes in gene expression and DNA methylation in patients receiving amoxicillin, while few changes were observed in patients receiving placebo. After 100 days of amoxicillin treatment, 28 genes were significantly differentially expressed, including the downregulation of 19 immunoglobulin genes. At one-year follow-up, the expression levels were still not completely restored. The significant changes in DNA methylation (n = 4548 CpGs) were mainly increased methylation levels between 100 days and one-year follow-up. Hence, the effects on gene expression occurred predominantly during treatment, while the effects on DNA methylation occurred after treatment. In conclusion, unrecognized side effects of long-term amoxicillin treatment were revealed, as alterations were observed in both gene expression and DNA methylation that lasted long after the end of treatment.

show abstract

Correlation between gene expression and MRI STIR signals in patients with chronic low back pain and Modic changes indicates immune involvement

Cited by 6 publications

References 73 publications

Using Machine Learning to Predict Surgical Site Infection After Lumbar Spine Surgery

Using Machine Learning to Predict Surgical Site Infection After Lumbar Spine Surgery

Modic changes as seen on MRI are associated with nonspecific chronic lower back pain and disability

Long-Term Use of Amoxicillin Is Associated with Changes in Gene Expression and DNA Methylation in Patients with Low Back Pain and Modic Changes

Contact Info

Product

Resources

About