Correlation between genomic analyses with metatranscriptomic study reveals various functional pathways of<i>Clostridium sartagoforme</i>AAU1, a buffalo rumen isolate

Nathani, Neelam M.; Duggirala, Srinivas M.; Bhatt, Vaibhav D.; Patel, Amrutlal K.; Kothari, Ramesh; Joshi, Chaitanya G.

doi:10.1080/09712119.2015.1091346

Cited by 9 publications

(8 citation statements)

References 31 publications

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“…The C . sartagoforme isolated from the rumen of buffalo is contrary to the results of this experiment [ 10 ], which may be due to the fact that many physiological and biochemical characteristics of bacteria are encoded by extrachromosomal genetic factors. The factors that affect the expression of physiological and biochemical characteristics are more complicated [ 12 ]; therefore, the results were different.…”

Section: Discussioncontrasting

confidence: 86%

“…Zhang JN isolated Clostridium sartagoforme FZ11 from cow dung compost, using urea as a nitrogen source and raw corn stalk as a substrate, confirming that this strain could decompose cellulose and have good hydrogen production characteristics [ 7 ]. In the research of Nathani Neelam M and and his coleagues, Clostridium sartagoforme AAU1 was isolated from Surti buffalo rumen liquor, and they pointed out that VFAs produced by the bacteria play an important role in animal health maintenance through genomic analysis [ 10 ]. At present, the research about C .…”

Section: Introductionmentioning

confidence: 99%

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Isolation, identification, and biological characteristics of Clostridium sartagoforme from rabbit

Gong

Wang

et al. 2021

PLoS ONE

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In order to develop microbial additives for rabbit feed, a spore-forming bacteria was isolated from the feces of Hyla rabbit using reinforced clostridium medium (RCM). The 16S rDNA sequence of the bacterium was subjected to pairwise sequence alignment using BLAST; the colony morphology, and physiological, biochemical, and stress resistance were studied. The results showed that the bacterium was Clostridium sartagoforme, a gram positive anaerobe, which can produce spores. The colony diameter was 0.5 mm—2.5 mm, the diameter of the bacteria was 0.5 μm—1.0 μm × 2.0 μm—6.3 μm, and the spore diameter was 1 μm—1.2 μm × 1 μm—1.2 μm. C. sartagoforme can utilize various sugars and alcohols such as fructose, galactose, sorbitol, and inositol. It secreted cellulase into the extracellular environment to form a transparent hydrolysis circle in Congo red medium, it could not liquify gelatin, and the lysine decarboxylase reaction was positive. In liquid medium it entered the stable growth period after 9 h of inoculation. Additionally, it had good stress resistance with a survival rate that exceeded 53% after gastric juice (pH 2.5) treatment for 3 h, it grew in a medium with a bile salt concentration of 0.3%, and the survival rate exceeded 85% after 10 minutes at 80°C. Moreover, animal testing indicated that this strain has no adverse effects on the morbidity and mortality of rabbits. In summary, C. sartagoforme XN-T4 was isolated from rabbit feces. This bacterium has good resistance to stress, can decompose a variety of monosaccharides and polysaccharides including cellulose, which is relatively harmless for animal health.

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Section: Discussioncontrasting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Isolation, identification, and biological characteristics of Clostridium sartagoforme from rabbit

Gong

Wang

et al. 2021

PLoS ONE

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“…The appearance of C. ghonii and C. sartagoforme in 2-h wilted silages might suggest that they are low water activity-tolerant. Protein degradation and butyric acid production characterisations were also observed in C. ghonii and C. sartagoforme (Hashimi et al 2013;Zhang et al 2015;Nathani et al 2016). The findings reported here might possibly point to a more important role for C. ghonii and C. sartagoforme, their uniform distribution indicated that C. ghonii and C. sartagoforme could be attributed mainly to the clostridial fermentation in 2-h wilted silage.…”

Section: Discussionmentioning

confidence: 49%

“…In contrast, C. ghonii and C. sartagoforme were still found in 2-h wilted silage. C. ghonii and C. sartagoforme have been found in soil, marine sediments, rumens, soft-tissue infections in humans and human faeces (Cano et al 2000;Nathani et al 2016), but have never been detected in silage. From this study, however, alfalfa silage can be regarded as a habitat of C. ghonii and C. Sartagoforme.…”

Section: Discussionmentioning

confidence: 99%

The effect of cultivar, wilting and storage period on fermentation and the clostridial community of alfalfa silage

Zheng

Niu

Zuo

et al. 2017

Italian Journal of Animal Science

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The objective of this study was to gain deeper insights into chemical transformations and the clostridial community dynamics during the ensiling of alfalfa. A factorial experiment was conducted with four alfalfa cultivars [Sanditi (A1), AC Caribou (A2), WL319HQ (A3) and 4030 (A4)] Â three wilting durations [0 h (direct-cut), 2 h and 4 h] Â three storage periods (14, 28 and 56 days). The clostridial community was examined using denaturing gradient gel electrophoresis. High butyric acid and ammonia nitrogen (NH 3 -N) contents and clostridia numbers were observed in direct-cut silage, with higher values observed in A4 than in the other three cultivars. However, butyric acid and NH 3 -N contents and clostridia numbers decreased with wilting regardless of the cultivar. Although Clostridium ghonii and Clostridium sartagoforme were common to all direct-cut and wilted silages, bands for these species were faint. Differences in the appearing time of these species were observed, bands for C. ghonii were found after 14 and 28 days of ensiling, while those for C. sartagoforme were only found after 56 days. In addition, in direct-cut silage, distinct bands for Clostridium perfringens were detected in A1, A2 and A3, while those for Clostridium sporogenes were detected in A4. The inactive spores of clostridia could be observed in 4-h wilted silage as no butyric acid was detected. It was concluded that the enhanced clostridial fermentation of direct-cut silage was attributed mainly to C. perfringens and C. sporogenes, while C. ghonii and C. sartagoforme were involved in the restricted clostridial fermentation of 2-h wilted silage. ARTICLE HISTORY

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“…However, the marine microbes are less explored as >90% are uncultivable in the laboratory conditions, making it inevitable to rely on the advanced 'omic' technologies to uncover the dearth of information on the genetic diversity of the marine microbes. s Metagenomics, that includes direct nucleic acid study of the total environment, has led to identification of novel microbes 2,3 potential metabolic pathways, 4 and several highly expressing enzymes 5 for varied applications apart from the general information about the microbial community and functional structure of the respective niches. 6 Thus, metagenomics and genomics based research studies are increasing for exploration of the marine microbes.…”

Section: Introductionmentioning

confidence: 99%

Sedimentomics - Exploring the Microbial Treasures in Deep Marine Environments

Nathani

Shekar

Dave

2016

JIG

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Advancements in metagenomics and related molecular approaches has allowed easy access to extreme and less explored vicinities over the globe, one of the best examples being the deep marine sediment microbial communities. Several recent investigations have provided access to high number of genomic and metagenomic datasets from circumnavigation in different water bodies that form a major portion of the Earth's surface area. And these microbes play a significant role in the biogeochemical cycles, biodegradation of hydrocarbons and most importantly help in elucidating the vast arena of antibiotics and antibiotic resistance that poses immense health risk to humans. Here, we summarize the advancements in deep sediment microbiome studies using the '-omic' technologies and the remarkable functional and community observations that have enriched the marine microbes' database in an extremely short span of time.

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Correlation between genomic analyses with metatranscriptomic study reveals various functional pathways ofClostridium sartagoformeAAU1, a buffalo rumen isolate

Cited by 9 publications

References 31 publications

Isolation, identification, and biological characteristics of Clostridium sartagoforme from rabbit

Isolation, identification, and biological characteristics of Clostridium sartagoforme from rabbit

The effect of cultivar, wilting and storage period on fermentation and the clostridial community of alfalfa silage

Sedimentomics - Exploring the Microbial Treasures in Deep Marine Environments

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