Correlation between liver metastasis of the colocalization of actin‐related protein 2 and 3 complex and WAVE2 in colorectal carcinoma

Iwaya, Keiichi; Oikawa, Kosuke; Semba, Seitaro; Tsuchiya, Benio; Mukai, Yasuo; Otsubo, Toshiya; Nagao, Toshiyasu; Izumi, Mitsuru; Kuroda, Masahiko; Domoto, Hideharu; Mukai, Kiyoshi

doi:10.1111/j.1349-7006.2007.00488.x

Cited by 74 publications

(66 citation statements)

References 40 publications

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“…Of some note are the CDCP1/ PKCy transmembrane signaling complex involved in survival (25), the E-cadherin/catenins complexes in mesenchyme induction and cell motility (2), and proteins of the ERM family in cell invasion (26). Additionally, Src targets the Wave2 and the a6/h4 integrin complexes, recently identified as novel players of epithelial cell motility/invasion (27,28). We also noticed a subgroup of signaling proteins that regulate the small GTPases of the Rho family, and which may modulate actinic cytoskeleton occurring during cell invasion (26).…”

Section: Resultsmentioning

confidence: 99%

Quantitative Phosphoproteomics Reveals a Cluster of Tyrosine Kinases That Mediates Src Invasive Activity in Advanced Colon Carcinoma Cells

et al. 2009

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The nonreceptor tyrosine kinase Src is frequently overexpressed and/or activated in human colorectal carcinoma (CRC), and its increased activity has been associated with a poor clinical outcome. Src has been implicated in growth and invasion of these cancer cells by still not well-known mechanisms. Here, we addressed Src oncogenic signaling using quantitative phosphoproteomics. Src overexpression increased growth and invasiveness of metastatic SW620 CRC cells. Stable isotope labeling with amino acids in cell culture in combination with liquid chromatography tandem mass spectrometry allowed the identification of 136 proteins which exhibited a significant increase in and/or association with tyrosine phosphorylation upon Src expression. These mainly include signaling, cytoskeleton, and vesicular-associated proteins. Interestingly, Src also phosphorylated a cluster of tyrosine kinases, i.e., the receptors Met and EphA2, the cytoplasmic tyrosine kinase Fak, and pseudo-tyrosine kinase SgK223, which were required for its invasive activity. Similar results were obtained with metastatic Colo205 CRC cells that exhibit high endogenous Src activity. We concluded that Src uses a tyrosine kinases network to promote its invasive activity in CRC and this implicates a reverse signaling via tyrosine kinase receptors. Targeting these tyrosine kinases may be of significant therapeutic value in this cancer. [Cancer Res 2009;69(6):2279-86]

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Section: Resultsmentioning

confidence: 99%

Quantitative Phosphoproteomics Reveals a Cluster of Tyrosine Kinases That Mediates Src Invasive Activity in Advanced Colon Carcinoma Cells

et al. 2009

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“…The activity of the Arp2/3 complex itself is controlled by actin nucleation-promoting factors, such as the N-WASP or the Scar/WAVE complexes which are themselves recruited to and activated at the membrane by Rac1 [172][173][174]. Increased expression of Arp2/3 and WAVE2 has been shown to correlate with poor prognosis in breast and liver carcinomas underlining the relevance of lamellipodia-like structures in cancer progression [175,176]. Furthermore, the formation of lamellipodia is also observed upon ErbB2-driven EMT in epithelial cells, indicating that the formation of lamellipodia-like structures underlies the increased invasiveness observed during EMT [177].…”

Section: Lamellipodia and Filopodiamentioning

confidence: 99%

EMT, the cytoskeleton, and cancer cell invasion

Yilmaz

Christofori

2009

Cancer Metastasis Rev

1,549

1,314

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The metastatic process, i.e. the dissemination of cancer cells throughout the body to seed secondary tumors at distant sites, requires cancer cells to leave the primary tumor and to acquire migratory and invasive capabilities. In a process of epithelial-mesenchymal transition (EMT), besides changing their adhesive repertoire, cancer cells employ developmental processes to gain migratory and invasive properties that involve a dramatic reorganization of the actin cytoskeleton and the concomitant formation of membrane protrusions required for invasive growth. The molecular processes underlying such cellular changes are still only poorly understood, and the various migratory organelles, including lamellipodia, filopodia, invadopodia and podosomes, still require a better functional and molecular characterization. Notably, direct experimental evidence linking the formation of migratory membrane protrusions and the process of EMT and tumor metastasis is still lacking. In this review, we have summarized recent novel insights into the molecular processes and players underlying EMT on one side and the formation of invasive membrane protrusions on the other side.

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“…WAVE2 expression is elevated in human hepatocellular carcinoma, which correlates with a poor prognosis (11). In addition, colocalization of WAVE2 and the Arp2 is an independent risk factor for liver metastasis originating from colorectal carcinoma (12), and enhancement of WAVE2 expression facilitates the invasion of breast cancer cell line MDA-MB-231 (13). Recently, Yao et al (14) found that WAVE2 was over expressed in gastric cancer cells and there was a significant correlation between WAVE2 protein levels and the migration/invasion of gastric cancer cell lines.…”

Section: Discussionmentioning

confidence: 99%

Flow Cytometry Method Analysis of Apoptosis

Nie²,

Hai-ying

et al. 2014

Technol Cancer Res Treat

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Flow cytometry method (FCM) is a generally accepted tool to analyze apoptosis. Although apoptosis assay kit was applied by many companies, the manufacturers were not consistent with whether using Trypsin with EDTA to collect the adherent cells. In another words, the influence of EDTA on apoptotic ratio is not clear. In this work, we compared the proportion of apoptotic cells with EDTA or EDTA-free Trypsin treatment by FCM. We concluded that Trypsin with or without EDTA has little influence on the proportion of apoptotic cells. In addition, we found that the ratio of necrosis and apoptosis was different in cells collected by scraping.

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Correlation between liver metastasis of the colocalization of actin‐related protein 2 and 3 complex and WAVE2 in colorectal carcinoma

Cited by 74 publications

References 40 publications

Quantitative Phosphoproteomics Reveals a Cluster of Tyrosine Kinases That Mediates Src Invasive Activity in Advanced Colon Carcinoma Cells

Quantitative Phosphoproteomics Reveals a Cluster of Tyrosine Kinases That Mediates Src Invasive Activity in Advanced Colon Carcinoma Cells

EMT, the cytoskeleton, and cancer cell invasion

Flow Cytometry Method Analysis of Apoptosis

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