Correlation between MBL2/CD14/TNF-α gene polymorphisms and susceptibility to spinal tuberculosis in Chinese population

Abstract

Objective: The present study investigated the clinical significance of mannose-binding lectin 2 (MBL2), cluster of differentiation 14 (CD14) and tumour necrosis factor-α (TNF-α) gene polymorphisms in patients with spinal tuberculosis (TB) in Chinese population. Methods: A total of 240 patients with spinal TB were enrolled in the present study from May 2013 to August 2016 at Hangzhou Red Cross Hospital. A total of 150 age- and sex-matched healthy subjects were enrolled as controls. The genomic DNA was extracted from the peripheral blood of all subjects, and the MBL2, CD14 and TNF-α gene polymorphisms were detected by direct DNA sequencing. Results: (1) Compared with controls, patients with spinal TB exhibited a significantly higher frequency of the XY genotype at the −221G>C polymorphism as well as the Q allele and PQ genotype or an association with the QQ genotype at the +4C>T polymorphism in the MBL2 gene. (2) Compared with controls, patients with spinal TB exhibited a significantly higher frequency of the T allele and TT genotype or an association with the CT genotype at the −159C>T polymorphism in the CD14 gene. (3) Compared with controls, patients with spinal TB exhibited a significantly higher frequency of the T allele and the CT genotype or an association with the TT genotype at the TNF-857 polymorphism in the TNF-α gene. Conclusion: The −221G>C polymorphism of MBL2, the −159C>T polymorphism of CD14 and the TNF-857 polymorphism of TNF-α are risk factors for spinal TB and may be involved in the development of spinal TB in the Chinese population. These factors are indicators of susceptibility to spinal TB and require clinical attention.

The Influence of Single Nucleotide Polymorphisms of NOD2 or CD14 on Susceptibility to Tuberculosis: A Systematic Review

“…Also, analyzes of odds ratio (OR) revealed an increased risk of TB when this allele was present, in case of both pulmonary and extrapulmonary disease. Of note, three of such studies [21][22][23] reported this aforementioned association in Chinese participants, reinforcing the idea that this CD14 allele may indeed increase the risk of developing TB in this particular population.…”

“…1). Through the study selection process, 13 articles met the inclusion criteria and were included in the systematic review [3,9,[21][22][23][24][25][26][27][28][29][30][31]. The majority of the studies evaluated CD14 gene polymorphisms (n = 9), whereas four studies analyzed NOD2 polymorphisms.…”

“…The studies reported data on 7 CD14 SNPs: rs2569190 [3, 9, 21-27], rs2569191 [22,23], rs3138078 [22], rs2915863 [22], rs3138076 [22], rs5744455 [22] and rs5744454 [22]. Of the 9 publications which investigated this gene locus, six studies reported a signi cant association between the T allele of SNP rs2569190 and higher odds of TB [3,[21][22][23][24]26]. The T allele was signi cantly more frequent in TB patients compared with healthy controls in subpopulations from China [21][22][23], Mexico [3], Iran [24] and South Korea [26].…”

“…Of the 9 publications which investigated this gene locus, six studies reported a signi cant association between the T allele of SNP rs2569190 and higher odds of TB [3,[21][22][23][24]26]. The T allele was signi cantly more frequent in TB patients compared with healthy controls in subpopulations from China [21][22][23], Mexico [3], Iran [24] and South Korea [26]. Also, analyzes of odds ratio (OR) revealed an increased risk of TB when this allele was present, in case of both pulmonary and extrapulmonary disease.…”

Association of human leukocyte antigens‐DQB2/DPA1/DPB1 polymorphism and pulmonary tuberculosis in the Chinese Uygur population

“…As mentioned in the literature, GA genotype of SNP rs2069837 A>G located within IL‐6 was associated with PTB, while GG genotype did not show any association with TB (Wu et al, ). Prior studies have noted the relationship between CT genotype of TNF‐857 and spinal TB, while TT genotype did not correlate with spinal TB (Zheng et al, ). Our research shows that heterozygous genotypes are meaningful; however, a number of studies have shown a significant evidence of homozygosity.…”