Correlation between membrane potential, spike discharge and tension in smooth muscle

Bülbring, Edith

doi:10.1113/jphysiol.1955.sp005299

Cited by 342 publications

(64 citation statements)

References 19 publications

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“…On the other hand, with strong currents, only anodal stimulation, which depolarized the cells far away, produced a spike. This phenomenon of reversed polarity seemed to be the same as that observed by Bulbring (1955) In each row, the three records on the left a were taken from the same cell near the left stimulating electrode, the three middle records b from a cell near the centre of the tissue, and the three right records c from a cell near the electrode on the right. Zero level indicated on the left of each group of records.…”

Section: Resultssupporting

confidence: 68%

Electrical responses of smooth muscle to external stimulation in hypertonic solution

Tomita¹

1966

The Journal of Physiology

142

107

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SUMMARY1. The electrical responses of single smooth muscle cells of the guineapig taenia coli to external stimulation were studied in two times hypertonic solution and compared with the responses to intracellular stimulation.2. Exposure to Krebs solution made two times hypertonic by adding sucrose abolished the mechanical movement and stopped the spontaneous electrical activity. The electrical response to stimulation was essentially similar to that in physiological solution.3. When the tissue was placed between stimulating electrodes, the cells near the cathode were depolarized and produced spikes, while the cells near the anode were hyperpolarized and produced small spikes only with weak stimuli. The cells near the centre were not polarized but produced spikes with a frequency pattern similar to that near the cathode.4. When both stimulating electrodes were put close together at one end of the tissue, the intracellularly recorded extrapolar polarization changed its polarity at 1-2 mm distance from the stimulating electrode. When an insulating partition was placed between the stimulating and recording site, the reversed polarity was no longer observed and the electrotonic potential spread decayed roughly exponentially with distance from the stimulating electrode. The time course of the electrotonic potential was similar to that predicted from the cable equation applied to nerve. The space constant was 1-68 + 0-08 mm (S.E. of mean) and the time constant was 60-100 msec. The cable properties may be explained by assuming that many fibres, connected in series and in parallel, are aggregated as functional units.5. The strength-duration curve was a simple hyperbola and the chronaxie was about 20 msec. The relation between extracellularly applied current and intracellularly recorded potential showed that membrane resistance decreased with depolarization and slightly increased with EXTERNAL STIMULATION OF SMOOTH MUSCLE 451 hyperpolarization. The spike was propagated in both directions at the same speed as in physiological solution (7.3 + 0 7 cm/sec).6. Long anodal current often produced electrical activity of low amplitude which seemed to be due to the spike activity near the cathode, because the same frequency modulation was seen in both activities, and external hyperpolarization reduced the size of the propagated spike.

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Section: Resultssupporting

confidence: 68%

Electrical responses of smooth muscle to external stimulation in hypertonic solution

Tomita¹

1966

The Journal of Physiology

142

107

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“…The stretch-induced reduction in membrane potential has also been reported on various excitable tissues such as vertebrate muscle spindle (KATZ, 1950), invertebrate stretch receptor (EYZAGUIRRE and KUFFLER, 1955a, b), vertebrate myelinated nerve (GRAY and RITcHIE, 1954), vertebrate skeletal muscle fibers (IsHIKo and SATO, 1960), and rhesus ape cardiac muscles (KAUFMANN and THEOPHILE, 1967). The frequency of repetitive discharges in vertebrate smooth muscle can also be increased by stretch with accompanying reduction in membrane potential (BULBRING, 1955). The rate of spontaneous firing in vertebrate skeletal muscle immersed in Ca-free medium also increases by stretch (BULBRING et al, 1956).…”

Section: Discussionmentioning

confidence: 82%

Length-dependent changes of pacemaker frequency in the isolated rabbit sinoatrial node.

1984

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To correlate changes in pacemaker frequency with those of length and tension in mammalian atrial tissues, a strip of the sinoatrial (SA) nodal tissue (about 10 mm in length and 4 mm in width) isolated from the rabbit heart was subjected to constant-length and constantload stretches, and the relation between the resulting pacemaker frequency changes and the segmental length changes of the preparation was examined by means of cinematographic recording of the preparation with carbon markers on its surface. The amount of stretch-induced length changes was larger in the perinodal tissue segments than in the SA nodal segments, indicating that the nodal area is less extensible than the perinodal area. The time course of stretch-induced length changes of one nodal segment (closer to the inferior versa cava) was found to roughly parallel that of pacemaker frequency changes, suggesting that the pacemaker frequency is primarily dependent on the length but not on the tension of the SA nodal area.

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“…Lengths from 4 to 6 mm were mounted isometrically in an organ bath of 3 ml., made of Perspex, through which solution flowed continuously at the rate of 2-3 ml./min, at a constant temperature of 350 C. The membrane potential was measured with intracellular electrodes with a resistance between 20 and 40 mQ, by the 'floating' method described by Woodbury & Brady (1956). Tension was measured with a mechano-electronic transducer valve (RCA 5734) mounted in the manner described by Bulbring (1955). The upper surface of the taenia was kept less than 2 mm below the surface of the bathing solution.…”

Section: Methodsmentioning

confidence: 99%

The influence of potassium, sodium and chloride on the membrane potential of the smooth muscle of taenia coli

Kuriyama¹

1963

The Journal of Physiology

106

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Correlation between membrane potential, spike discharge and tension in smooth muscle

Cited by 342 publications

References 19 publications

Electrical responses of smooth muscle to external stimulation in hypertonic solution

Electrical responses of smooth muscle to external stimulation in hypertonic solution

Length-dependent changes of pacemaker frequency in the isolated rabbit sinoatrial node.

The influence of potassium, sodium and chloride on the membrane potential of the smooth muscle of taenia coli

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