Correlation between p53 and Mdm2 expression with histopathological parameters in cattle squamous cell carcinomas

Al-Jameel, Waseem; Al-Mahmood, Saevan S.; Al-Saidya, A. M.

doi:10.14202/vetworld.2022.10-15

Cited by 3 publications

(2 citation statements)

References 27 publications

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“…Finally, slides were dehydrated in ethanol and xylene and coverslipped. Slides were inspected using a microscope and photographed using a digital camera to assess the degree of gene expressions (17)(18)(19).…”

Section: Immunohistochemistrymentioning

confidence: 99%

Inflammatory mediators and inflammatory cells as reliable molecular targets for assessment of wound age and vitality in rats

Al-Salih

Al-Jameel

2023

IJVS

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Evaluation of the wound age and vitality is a persistent problem for forensic examinations to determine when injuries occurred and whether during the antemortem or post-mortem period. This study aimed to use the inflammatory cells and mediators as a target marker to determine wound age in antemortem or post-mortem samples in rats. Wound skin samples (n=39) were collected from antemortem groups aged 30, 60, 180, and 360 min and post-mortem groups within the same periods with control samples (unwounded group). Immunohistochemical staining examined all tissue samples for two inflammatory cell markers (CD15, CD68) and 2 inflammatory mediators (IL-6, TNF-α). The results showed that CD15-positive neutrophils were expressed in all time intervals of antemortem, and the expression gradually increased from 30 to 360 min. The CD68-positive macrophages began at 30 minutes and reached the highest level at 360 minutes in the antemortem wound. Because of the negative expression of CD15 and CD68 in all postmortem groups, they could be a marker for comparing the vital and non-vital wounds. Furthermore, the Immunoreactivity results showed that the IL-6 and TNF-α were negatively expressed in unwounded skin samples. In addition, the periods after the induced wound produced a rise in the expression area of both markers in antemortem wounds. In this study, IL-6 and TNF-α expressions were negative or low in areas far from wound margin, unwounded specimens, and post-mortem wounds. CD15, CD68, IL-6, and TNF-α may be valuable markers for assessing wound age and vitality.

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Section: Immunohistochemistrymentioning

confidence: 99%

Inflammatory mediators and inflammatory cells as reliable molecular targets for assessment of wound age and vitality in rats

Al-Salih

Al-Jameel

2023

IJVS

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“…The sections were stained with haematoxylin for 60 second, washed in distal water, dehydrated and cover-slipped. Sections were seen using a microscope and photographed using digital camera (13).…”

Section: Immunocytochemistrymentioning

confidence: 99%

Immunohistochemical expression of proliferation markers in canine osteosarcoma

Al-Jameel¹,

Al-Sabaawy²,

Abed³

et al. 2022

IJVS

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Canine osteosarcoma is an extremely malignant bone tumor that often arises in the bones of the limbs. It is a highly metastatic disease distinguished by proliferative bone lesions and a tendency for pulmonary metastasis. Overexpression of proliferative proteins are associated with bad prognosis in human osteosarcoma. Here, we tested the expression of the different proliferative proteins (p53, p16, vimentin, and mdm2) in nine archival samples with canine osteosarcoma. Paraffin-embedded tissue sections were confirmed by histopathology and stained by immunohistochemistry for p53, p16, vimentin and mdm2. Positive expression of these proteins was evaluated as the ratio of positive cancer cells and the intensity staining was assessed in several areas. Histopathologically, 95% of samples were grade II and III. All high-grade osteosarcomas were particularly cellular. The cancer cells were generally large spindle-shaped and large nucleus with distribution of osteoid between the cancer cells. Immunohistochemical detection of p53, p16, vimentin and mdm2 was 89%, 56%, 78%, and 89% of samples respectively. The staining intensity for p53, p16, vimentin and mdm2 was particularly nuclear in 81%, 66%, 78%, and 79% of the cancer cells respectively. Our present work suggests that p53, p16, vimentin, and mdm2 were detected in grade III canine osteosarcomas samples. In addition, these proliferative markers are the significant biomarkers in canine osteosarcomas and can be used as a predictor for diagnostic and prognostic value and allowing cancer differentiation. This primary data supports that both canine and human osteosarcomas share same molecular characters which are approved by expression of proliferative genes.

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Carcinoma escamocelular ocular en bovino de raza Brahman Gris: reporte de caso

Quebrada,

Ramírez

2023

Rev. Med. Vet. Zoot.

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En el presente trabajo se reporta el primer caso de un bovino de raza Brahman de 5 años, el cual se remitió por presentar una masa en el tercer parpado en el ojo izquierdo que obstruía la visión. El análisis histopatológico de la muestra remitida evidencia un carcinoma de células escamosas bien diferenciado sin evidencia de invasión linfovascular. El manejo terapéutico consistió en exenteración para evitar recidivas, por lo cual la evolución del paciente fue satisfactoria sin causar problemas en su rendimiento productivo. Se ha descrito que un posible factor de riesgo está relacionado con una permanente exposición a la luz solar que afecta la replicación del ADN de las células e induce procesos tumorales. En ese sentido, es indispensable prestar atención y realizar monitoreo a las anormalidades que se puedan presentar en el ganado ya sea cebuino o taurino.

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Correlation between p53 and Mdm2 expression with histopathological parameters in cattle squamous cell carcinomas

Cited by 3 publications

References 27 publications

Inflammatory mediators and inflammatory cells as reliable molecular targets for assessment of wound age and vitality in rats

Inflammatory mediators and inflammatory cells as reliable molecular targets for assessment of wound age and vitality in rats

Immunohistochemical expression of proliferation markers in canine osteosarcoma

Carcinoma escamocelular ocular en bovino de raza Brahman Gris: reporte de caso

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