Correlation Between Phenotypic and Genotypic Identification of Trichophyton Violaceum

Abstract

Background: Trichophyton violaceum is an anthropophilic dermatophyte. It is the most common etiologic agent causing dermatophytosis especially tinea capitis in Egypt. As routine phenotypic identification of dermatophytes is either slow or lack specificity, improved identification methods are required. The application of PCR has made rapid and precise identification of T. violaceum possible. Aim: This study was designed to correlate between phenotypic and genotypic identification of T. violaceum. Subjects: Two hundred patients with tinea capitis (122 males and 78 females) with a mean age of 8.5±3.1 were the subjects of this study. Methods: Phenotypic identification of T. violaceum determined by observation of its macroscopic and microscopic characteristics and culture on differential media was correlated with ITS-based PCR. Results: Genotypic identification results of T. violaceum in comparison with phenotypic identification results were identical. Conclusion: Although there is no difference between the results of phenotypic and genotypic identification methods of T. violaceum isolates, genotypic method is recommended as it is rapid and accurate.

Antifungal Activity of Petroleum Ether and Ethanol Extracts of Moringa Oleifera Seeds

“…From 70 samples collected from skin and nail infection; 20 dermatophyte isolates, 21 non dermatophyte moulds and 25 yeast were isolated.Concerning dermatophyte which isolated only from skin infections they were identified by macromorphological and micromorphological characters as well as cultures on rice grains, BCP medium and MHB medium into: Microsporum canis, Trichophyton violaceum, Trichophyton mentagrophytes and Trichophyton rubrum. M. canis and T.violaceum were isolated as the most predominant cause of Tinea capitis the result which coincide with previous works done by El Fangary et al (2011) and Soliman et al (2013). From Tinea corporis and tinea pedis T. rubrum, T.mentagrophytes , M. canis and T. violaceum were the most dermaophytes isolated the results which in agreement with El Fangary et al, (2011) and .…”