Correlation between plasma membrane surface area and transferrin secretion rate in isolated hepatocytes

Pechinot, Danielle; Foucrier, Jean; Feldmann, Gérard

doi:10.1002/jcp.1041290308

Cited by 6 publications

(11 citation statements)

References 27 publications

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“…At the present time, the tech nique has been used only to study plasma protein secretion by a global population of isolated hepatocytes and not by hepatocytes isolated from a particular lobular zone. We have demonstrated that the rate of secretion for several plasma proteins, such as albumin [112,113], fibrinogen [113] or transferrin [114], varies from hepatocyte to hepatocyte and increased with the cell size [112,114]. It is difficult to transpose these results in vivo since the size of individual hepatocytes has not been measured in situ.…”

Section: Hepatocellular Heterogeneity For Plasma Protein Synthesismentioning

confidence: 97%

Functional Hepatocellular Heterogeneity for the Production of Plasma Proteins

Feldmann

Scoazec²,

Racine³

et al. 1992

Enzyme

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It is now well established that hepatocytes are the main liver cells responsible for the synthesis of plasma proteins produced by the liver. That these cells are not specialized in the production of the different plasma proteins is also well established. Presently the point still debated is whether a functional hepatocellular heterogeneity exists for plasma protein synthesis as for many other hepatocyte functions. Several physiological and pathological situations suggest that this heterogeneity takes place in the hepatocytes of two opposite hepatic lobular zones, the periportal and centrilobular zones. However, this zonal difference, which supposes different regulatory mechanisms, must be confirmed by techniques other than the now classical immunocytochemistry or the in situ hypbridization technique recently proposed for the demonstration of mRNAs in hepatocytes. Another hepatocellular heterogeneity, the intercellular heterogeneity, which can be observed in the same lobular zone, is more difficult to analyze, but shows that from hepatocyte to hepatocyte a variation exists in the synthesis of a given plasma protein.

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Section: Hepatocellular Heterogeneity For Plasma Protein Synthesismentioning

confidence: 97%

Functional Hepatocellular Heterogeneity for the Production of Plasma Proteins

Feldmann

Scoazec²,

Racine³

et al. 1992

Enzyme

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“…Even though this determination did not include the change in microvilli surface area or membrane blebbing observed, the results 55 suggest that membrane area may not be a ratedetermining factor in hybridoma respiratory metabolism (Clegg and Gordon, 1985) or Ig secretion. This is in contrast to the correlation between plasma membrane surface area and transferrin secretion rate in isolated hepatocytes (Pechinot et al, 1986) and recent implications that the degree of hybridoma microvilli density may be related to MoAb secretion (Kitano et al, 1988).…”

Section: Discussionmentioning

confidence: 56%

“…As cell volume changes, surface membrane area, membrane recycling (Novak et al, 1988) and the distribution of blebs and microvilli may also change. Membrane blebbing and the density of microvilli may be related to available reserve surface membrane for rapid nonmetabolic expansion of cell volume (Erickson and Trinkaus, 1976;Borrelli et al, 1986;Clegg, 1986), nutrient uptake and protein secretion rate in constitutively secreting cells (Pechinot et al, 1986). Hybridoma morphology is intimately related to cell cytoskeletal filaments and subcellular organelles.…”

Section: Introductionmentioning

confidence: 99%

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Methods for determination of growth-rate-dependent changes in hybridoma volume, shape and surface structure during continuous recycle

1990

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Hybridoma volume and surface membrane structure were found to vary as a function of specific growth rate using a method of cell recycle with continuous medium perfusion to vary growth rate. Mean hybridoma volume determined at constant osmolality by both electronic particle counting and scanning electron microscopic (SEM) methods indicated that rapidly growing cells are significantly larger than very slowly growing cells. We have previously determined that during both rapid and slow growth over a range of L-glutamine provision rates (Gln PR) that specific monoclonal antibody (MoAb) secretion rate was not changed. In this study a constant MoAb secretion rate per unit of membrane area was found which may indicate that changing membrane area is not a rate-determining factor in MoAb secretion. SEM methods were of limited use for accurate determination of cell volume due to cell shrinkage and large coefficients of variations. In spite of this limitation, SEM stereology methods were useful in confirming that cells remained spherical over a wide range of specific growth rates and that hybridoma cells were not circular. Sequential SEM observations also revealed that surface membrane structure of the 9.2.27 murine hybridoma investigated was correlated with growth rate. Under conditions of very slow growth, hybridoma surface microvilli density appeared to be significantly reduced.

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“…Transferrin (Tf), like most other plasma proteins, is synthesized by hepatocytes [12,25,37] and its production in normal and/or pathological states has been extensively investigated [6,15,26,30,33]. We previously demonstrated at the single cell level that the amount of secreted Tf is proportional to the size of secreting hepatocytes and constant per unit plasma membrane surface area and per unit time [31]. The aim of the present work was to establish whether a relationship exists or not between Tf secretion and hepatocytic ploidy.…”

Section: Introductionmentioning

confidence: 99%

Transferrin secretion and hepatocyte ploidy: analysis at the single cell level using a semi‐automatic image analysis method

Foucrier

Pechinot

Rigaut

et al. 1988

Biology of the Cell

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In a previous work it was shown that transferrin (Tf) secretion is directly related to the membrane surface area of hepatocytes (Péchinot D. et al. [31]). The aim of the present work was to search for a possible relationship between Tf secretion and hepatocytic ploidy using a semi-automatic image analysis method. A determination of Tf secretion by isolated normal adult hepatocytes was achieved at the single cell level, using a modified reverse hemolytic plaque test. A Feulgen reaction was also performed on these hepatocytes. It allowed the evaluation, for each secreting hepatocyte, of the quantity of Tf secreted and its nuclear characteristics. Discrimination between diploid (2c) and tetraploid (4c and 2c2c) hepatocytes was performed and the amount of Tf secreted by each subpopulation determined. It appeared that a 2-fold secretion ratio was not found between tetraploid and diploid hepatocytes. These results suggest, as Tf production is not directly proportional to the degree of ploidy of hepatocytes, that some not yet elucidated regulatory mechanisms may act on Tf gene expression.

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Correlation between plasma membrane surface area and transferrin secretion rate in isolated hepatocytes

Cited by 6 publications

References 27 publications

Functional Hepatocellular Heterogeneity for the Production of Plasma Proteins

Functional Hepatocellular Heterogeneity for the Production of Plasma Proteins

Methods for determination of growth-rate-dependent changes in hybridoma volume, shape and surface structure during continuous recycle

Transferrin secretion and hepatocyte ploidy: analysis at the single cell level using a semi‐automatic image analysis method

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