Correlation between presynaptic dense bodies and tranmitter output at lobster neuromuscular terminals by serial section electron microscopy

Govind, C. K.; Chiang, R. Gary

doi:10.1016/0006-8993(79)90669-3

Cited by 66 publications

(38 citation statements)

References 22 publications

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“…We found the average DB height to be 60 nm and the mean surface area to be 0.029 pmz in both claws. This is in good agreement with other reported measurements for DB heights (55-75 nm in cockroach: Wood, Pfenninger, and Cohen, 1977;55-65 nm in lobster: Govind and Chiang, 1979). A summary of our results is given in the micrograph and diagram in Figure 4.…”

Section: Dense Body Structuresupporting

confidence: 94%

A comparative study by serial electron microscopy of neuromuscular junctions in the dimorphic claws of the snapping shrimp, Alpheus heterochelis

1982

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We compared the neuromuscular junctions on the main closer muscle in the first pair of chelipeds in the snapping shrimp Alpheus heterochelis by serial section electron microscopy. We sought an ultrastructural basis for the different behavioral and physiological functions of these dimorphic claws and for the role of the nervous system in claw transformation. We were unable to detect any statistically significant morphological differences between the junctions. Further, we found the muscle fiber populations and filament arrangements, as well as the electrical properties of the fibers, to be more homogeneous and similar to each other in A. heterochelis than those reported for another species, A. armillatus. We consider our results in light of recent data on the anatomy and electrical properties of the motor neurons within the CNS and conclude that the neural trigger for claw transformation involves factors not revealed by conventional electron microscopy.

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Section: Dense Body Structuresupporting

confidence: 94%

A comparative study by serial electron microscopy of neuromuscular junctions in the dimorphic claws of the snapping shrimp, Alpheus heterochelis

1982

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“…Active zones are structural entities on the presynaptic membrane where vesicles dock to fuse with the membrane. Such differences in synaptic complexity account for the differences between the Ib and Is varicosities in D. melanogaster (Atwood et al, 1993;Kurdyak et al, 1994) and for high and low output synapses in crustaceans Cooper, 1995, 1996a,b;Cooper et al, 1995aCooper et al, , 1996aGovind and Chiang, 1979;King et al, 1996). To resolve this issue in relation to synaptic efficacy in M. scalaris, electron micrographic studies of the motor nerve terminals will need to be undertaken.…”

Section: Discussionmentioning

confidence: 99%

Characterization of development, behavior and neuromuscular physiology in the phorid fly, Megaselia scalaris

Harrison

Cooper

2003

Comparative Biochemistry and Physiology Part A: Molecular &

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The Phoridae is known as 'scuttle flies' because they walk in rapid bursts of movement with short pauses between. In this study, larval locomotive behavior and development was characterized in the phorid, Megaselia scalaris. Comparison was made with the well-characterized fruit fly model, Drosophila melanogaster. Developmentally, the rate of maturation was consistently slower for Megaselia than Drosophila. This disparity was exaggerated at lower temperatures, particularly during larval development. In addition to slower growth, movements in Megaselia were also slower, as evidenced by reduced rates of larval body wall contractions and mouth hook movements. Megaselia larvae also displayed a unique behavior of swallowing air when exposed to a small pool of liquid. This permitted floating upon immersion and, therefore, might prevent drowning in the natural environment. The anatomical and physiological properties of a neuromuscular junction in the phorid larvae were also examined. The innervation of the motor nerve terminals on the ventral abdominal muscle (m6) is innervated by Type Ib and Is axons, similar to Drosophila. As in Drosophila, the Is terminals produce larger excitatory postsynaptic potentials (EPSPs) than the Ib. The amplitudes of the EPSPs in M. scalaris were reduced compared to those of D. melanogaster, but unlike D. melanogaster the EPSPs showed marked facilitation when stimulated with a 20 Hz train. We conclude that there may be differences in synaptic structure of the nerve terminals that could account for the different electrophysiological behaviors. ᮊ

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“…The "brute force" approach of serial sectioning and reconstruction from electron micrographs was adopted; this has proven useful in similar studies designed to clarify the innervation of crustacean muscles (Jahromi and Atwood, 1974;Atwood and Jahromi, 1977;Govind and Pearce, 1981;Govind and Chiang, 1979). We show in this paper that the two major axons supplying muscles 6 and 7 have distinctive endings, representing subcategories of Type I, whereas a structurally different type of ending, probably representing a subcategory of Type 11, can be readily found in muscle 12 and less frequently in muscle 13.…”

Section: Figurementioning

confidence: 93%

Differential ultrastructure of synaptic terminals on ventral longitudinal abdominal muscles in Drosophila larvae

1993

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The innervation of ventral longitudinal abdominal muscles (muscles 6, 7, 12, and 13) of third-instar Drosophila larvae was investigated with Nomarski, confocal, and electron microscopy to define the ultrastructural features of synapse-bearing terminals. As shown by previous workers, muscles 6 and 7 receive in most abdominal segments "Type I" endings, which are restricted in distribution and possess relatively prominent periodic terminal enlargements ("boutons"); whereas muscles 12 and 13 have in addition "Type II" terminals, which are more widely distributed and have smaller "boutons". Serial sectioning of the Type I innervation of muscles 6 and 7 showed that two axons with distinctive endings contribute to it. One axon (termed Axon 1) has somewhat larger boutons, containing numerous synapses and presynaptic dense bodies (putative active zones for transmitter release). This axon also has more numerous intraterminal mitochondria, and a profuse subsynaptic reticulum around or under the synaptic boutons. The second axon (Axon 2) provides somewhat smaller boutons, with fewer synapses and dense bodies per bouton, fewer intraterminal mitochondria, and less-developed subsynaptic reticulum. Both axons contain clear synaptic vesicles, with occasional large dense vesicles. Approximately 800 synapses are provided by Axon 1 to muscles 6 and 7, and approximately 250 synapses are provided by Axon 2. In muscles 12 and 13, endings with predominantly clear synaptic vesicles, generally similar to the Type I endings of muscles 6 and 7, were found, along with another type of ending containing predominantly dense-cored vesicles, with small clusters of clear synaptic vesicles. This second type of ending was found most frequently in muscle 12, and probably corresponds to a subset of the "Type II" endings seen in the light microscope. Type I endings are thought to generate the 'fast' and 'slow' junctional potentials seen in electrophysiological recordings, whereas the physiological actions of Type II endings are presently not known.

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Correlation between presynaptic dense bodies and tranmitter output at lobster neuromuscular terminals by serial section electron microscopy

Cited by 66 publications

References 22 publications

A comparative study by serial electron microscopy of neuromuscular junctions in the dimorphic claws of the snapping shrimp, Alpheus heterochelis

A comparative study by serial electron microscopy of neuromuscular junctions in the dimorphic claws of the snapping shrimp, Alpheus heterochelis

Characterization of development, behavior and neuromuscular physiology in the phorid fly, Megaselia scalaris

Differential ultrastructure of synaptic terminals on ventral longitudinal abdominal muscles in Drosophila larvae

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