Correlation between semen analysis by motile sperm organelle morphology examination and sperm DNA damage

Background Sperm DNA damage is common in infertile men and is associated with poor semen parameters but the impact of an isolated sperm abnormality on sperm DNA damage has not been studied. Objective To evaluate sperm DNA damage in a large cohort of infertile men with isolated sperm defects. Design, setting and participants Retrospective study of 1084 consecutive, non-azoospermic infertile men with an isolated sperm defect: isolated oligozoospermia (iOligo), isolated asthenozoospermia (iAstheno) or isolated teratozoospermia (iTerato). Outcome measurements and statistical analysis We examined and compared clinical parameters, conventional semen parameters and %sperm DNA fragmentation (%SDF, assessed by flow cytometry-based Terminal deoxynucleotidyl transferase-mediated dUTP Nick End-Labeling assay) in the three groups of men. Results and limitations The mean (±SD) %SDF was significantly higher in the iAstheno compared to the iOligo and iTerato groups (25.0±14.0 vs. 19.2±11.6 and 20.7±12.1 %, respectively, P<0.0001). Similarly, the proportion of men with high %SDF (>30 %) was significantly higher in the iAstheno compared to the iOligo and iTerato groups (31 % vs. 18 % and 19 %, respectively, P<0.0001). In the group of 713 men with iAstheno, %SDF was positively correlated with paternal age (r=0.20, P<0.0001) and inversely correlated with %progressive motility (r=−0.18, P<0.0001). In the subset of 218 men with iTerato, %SDF was also positively correlated with paternal age (r=0.15, P=0.018) and inversely correlated with %progressive motility (r=−0.26, P=0.0001). Conclusions In this large cohort of infertile men with isolated sperm abnormalities, we have found that the sperm DNA fragmentation level is highest in the men with sperm motility defects and that 31 % of these men have high levels of sperm DNA fragmentation. The data indicate that poor motility is the sperm parameter abnormality most closely related to sperm DNA damage.

Section: Discussionsupporting

confidence: 83%

Section: Discussionsupporting

confidence: 58%

Which isolated sperm abnormality is most related to sperm DNA damage in men presenting for infertility evaluation

Belloc¹,

Benkhalifa²,

Cohen-Bacrie³

et al. 2014

“…Nonetheless, there are many reports suggesting that sperm vacuoles reflect sperm chromatin packaging/DNA abnormalities [1,4,6,[10][11][12][13][14][15][16].…”

Section: Discussionmentioning

confidence: 99%

“…It has been suggested that sperm vacuoles should be regarded as normal features of the sperm head that occur naturally during the process of condensation of sperm nuclei, and should not be regarded as degeneration but as physiological changes that do not compromise the sperm quality [5,6]. Meanwhile, studies suggest that the presence of sperm vacuoles is related to male sub fertility [7], lower mitochondrial membrane potential [8], higher incidence of chromosomal abnormalities [8,9], and sperm chromatin packaging/DNA abnormalities [1,4,6,[10][11][12][13][14][15][16]. It has also been suggested that sperm vacuoles reflect non-reacted acrosome [17,18].…”

Section: Introductionmentioning

confidence: 99%

The prevalence of sperm with large nuclear vacuoles is a prognostic tool in the prediction of ICSI success

Setti

Braga

Vingris

et al. 2013

Purpose To investigate if there is a correlation between the prevalence of sperm with large nuclear vacuoles (LNV) and intracytoplasmic sperm injection (ICSI) outcomes. Methods Two hundred male patients undergoing ICSI had their sperm morphology evaluated through motile sperm organelle morphology examination (MSOME) and the percentage of LNV sperm was recorded and correlated to the ICSI outcomes.Results The percentage of sperm with LNV negatively influenced the blastocyst formation (S: 16.9, R 2 : 20.5 %, p=0.004) and implantation (S: 34.7, R 2 : 26.2 %, p=0.001). There were significant differences in the percentage of sperm with LNV between patients in which pregnancy was achieved or not (22.2 % vs. 28.4 %, p<0.001) and in patients with ongoing pregnancy or not (22.4 % vs. 28.5 %, p<0.001). The incidence of sperm with LNV was determinant to the decreased odds of pregnancy (OR: 0.74, p<0.001) and increased odds of miscarriage (OR: 1.46, p<0.001). The area under the curve (AUC) was sufficient to distinguish between couples which did achieve pregnancy or not (AUC: 0.922, p<0.001).Conclusions The MSOME is a prognostic tool in the prediction of ICSI success and could be used to select patients that should have their sperm selected by MSOME for ICSI.

“…This technique revealed a new morphological criterion in human spermatozoa: the presence of nuclear vacuoles. Several studies have found increased levels of fragmented DNA in spermatozoa with large vacuoles [2][3][4][5], whereas others [6,7] have shown abnormalities of chromatin condensation in such spermatozoa. Although the origin of these vacuoles raises many questions, some strong correlations have been established between the morphology of the spermatozoon, in particular the presence of large vacuoles, and its nuclear quality (degree of chromatin condensation and/or DNA integrity, chromosomal content) [8].…”

Section: Introductionmentioning

confidence: 99%

Intracytoplasmic morphologically selected sperm injection (IMSI) does not improve outcome in patients with two successive IVF-ICSI failures

Gatimel

Parinaud

Léandri

2016

Purpose Assessment of sperm morphology has been reconsidered since 2001 with the development of motile sperm organelle morphology examination (MSOME). This observation technique that combines high magnification microscopy and the Nomarski interference contrast makes it possible to select spermatozoa with as few vacuoles as possible before microinjection into the oocyte (intracytoplasmic morphologically selected sperm injection, IMSI). More than 10 years after the development of IMSI, the indications of the IMSI technique and its ability to increase pregnancy and/ or birthrates (compared with conventional ICSI) are still subject to debate. We aimed to better define the interest of IMSI in the third attempt. Methods We assessed the benefit of IMSI by carrying out a retrospective comparative study between IMSI and conventional ICSI during a third ART attempt. Two hundred sixteen couples with two previous ICSI failures were studied between February 2010 and June 2014. Results IMSI did not significantly improve the clinical outcomes compared with ICSI, either for implantation (12 vs 10 %), clinical pregnancy (23 vs 21 %), or live birth rates (20 vs 19 %).Conclusion This study provides supplementary arguments for not achieving IMSI procedure in the third attempt after two previous ICSI failures.