Correlation between Some Nutritional Components and the Total Antioxidant Capacity Measured with Six Different Assays in Eight Horticultural Crops

Abstract

The contents of antioxidant nutritional compounds, total soluble phenolics (TSP), vitamin C, vitamin E, beta-carotene, and total carotenoids (TC), were correlated with the total antioxidant capacity (AOC) of hydrophilic (HPE) and lipophilic extracts (LPE) from eight horticultural crops, namely, guava, avocado, black sapote, mango, papaya, prickly pear fruit, cladodes, and strawberry. AOC was measured using six different assays: 2,2'-diphenyl-1-picrylhydrazyl (DPPH), N,N-dimethyl-p-phenylendiamine (DMPD), ferric-ion-reducing antioxidant power (FRAP), oxygen radical absorbance capacity (ORAC), Trolox equivalent antioxidant capacity (TEAC), and total oxidant scavenging capacity (TOSC). AOC values from HPE were about 95 times higher than LPE values. HPE of guava had the highest AOC value when evaluated with DMPD, DPPH, FRAP, TEAC, and TOSC assays, whereas with ORAC assay, black sapote had the highest value. HPE of papaya and prickly pear fruit presented the lowest AOC values with all assays. From HPE, vitamin C and TSP contents were highly correlated with AOC for all assays, while from LPE, TC and beta-carotene contents possessed a high correlation with AOC only in the DMPD assay.

Calidad nutrimental y nutraceutica de hoja de moringa proveniente de árboles de diferente altura

“…Vitamina C. El ácido L-dehidro ascórbico presenta actividad biológica y puede ser convertido fácilmente a ácido L-ascórbico por los humanos, la suma de ácido ascórbico y L-dehidro ascórbico se toma como el contenido de vitamina C (Corral-Aguayo et al, 2008). El contenido de ácido ascórbico y dehidro ascórbico disminuyo en las hojas de los árboles conforme aumenta la altura (Cuadro 3).…”

“…Vitamin C. L-dehydro ascorbic acid shows biological activity and can be easily converted to L-ascorbic acid by humans, the amount of ascorbic acid and L-dehydro ascorbic is taken as the content of vitamin C (Corral-Aguayo et al, 2008). The content of ascorbic acid and dehydro ascorbic decreased in the leaves of trees as height increases (Table 3).…”

“…La vitamina C fue determinada por cromatografía líquida de alta resolución (HPLC) de acuerdo con el procedimiento descrito por Corral-Aguayo et al (2008). Brevemente, a una muestra de hoja (1 g) se le añadieron 10 mL de una solución de extracción (ácido cítrico 0.1 M y 0.05% de EDTA a pH 2.35-2.4), se agitó, centrifugó (5000 rpm, 10 min) y se recuperó el sobrenadante.…”

“…Vitamin C was determined by high performance liquid chromatography (HPLC) according to the procedure described by Corral-Aguayo et al (2008). Briefly, to a leaf sample (1 g) were added 10 ml of an extraction solution (citric acid 0.1 M and EDTA 0.05% at pH 2.35-2.4), stirred, centrifuged (5 000 rpm, 10 min) and the supernatant was recovered.…”

Effect of aguamiel (agave sap) on hematic biometry in rabbits and its antioxidant activity determination

“…Furthermore, these beverages reduced the risk of anaemia and iron deficiency anaemia in pregnant women and infants (Makola et al, 2003;Nnam, 2009). Some fruits or vegetables (nopalito-tender cactus, guava, papaya, mango, strawberry, prickly pear and others) grown in Mexico have been considered appropriate owing to their antioxidant properties (Corral-Aguayo et al, 2008). Additionally, in the arid and semiarid lands of Mexico, the pulp of a plant known as maguey manso (Agave salmiana, Gentry) is used as forage, while aguamiel (its sap) is collected in order to produce the alcoholic beverage known as pulque.…”