Correlation between spontaneous metastatic potential and type I collagenolytic activity in a human pancreatic cancer cell line (SUIT-2) and sublines

Abstract

A human pancreatic cancer cell line (SUIT-2) and four sublines cloned in vitro (S2-007, S2-013, S2-020 and S2-028) were inoculated into nude mice for assessment of metastatic potentials. After 16 weeks of subcutaneous injection, the parent SUIT-2 line metastasized to the lungs and lymph nodes in three of six mice. S2-007 cells presented the highest metastatic potential in pulmonary (5/6) and lymph node (2/6) metastases among the four sublines. No metastasis was found in S2-028. The incidence of spontaneous pulmonary metastasis was correlated with that of pulmonary colonization after intravenous (i.v.) injection of cell clusters (r = 0.87, P = 0.056). Pulmonary colonization potential using single cells, however, did not always reflect a spontaneous metastatic ability. Type I collagenolytic activity in serum-free conditioned media of these cells was correlated effectively with the incidence of spontaneous pulmonary metastasis (r = 0.92, P = 0.026) and pulmonary colonization after i.v. injection of cell clusters (r = 0.95, P = 0.013). Thus, type I collagenolytic activity may possibly be essential to spontaneous cancer metastasis.

Expression of collagenase (MMP2), stromelysin (MMP3) and tissue inhibitor of the metalloproteinases (TIMP1) in pancreatic and ampullary disease

“…The immunoreactivity of MMP3 and TIMPI also correlated, in keeping with previous studies (Wilhelm et al, 1989). It has been shown previously in human pancreatic cancer cells that expression of MMP1 correlated with spontaneous metastatic ability (Taniguchi et al, 1992). An increase in type I collagenolytic activity was noted in a subclone of the human pancreatic cancer cell line SUIT2 that demonstrated an increased metastasising ability in nude mice (Taniguchi et al, 1992).…”

“…It has been shown previously in human pancreatic cancer cells that expression of MMP1 correlated with spontaneous metastatic ability (Taniguchi et al, 1992). An increase in type I collagenolytic activity was noted in a subclone of the human pancreatic cancer cell line SUIT2 that demonstrated an increased metastasising ability in nude mice (Taniguchi et al, 1992). Reduced expression of TIMP is known to be associated with tumour cell line progression (Khokka et al, 1991) but evidence for this in human tumours has been lacking.…”

“…The cellular source of MMPs is often difficult to identify in complex tissues. MMP1 (type I collagenase) appears to be a product of fibroblasts (Biswas, 1982), some tumour cell lines, including the pancreatic cancer cell line SUIT2 (Taniguchi et al, 1992) and colonic tumours (Gray et al, 1993). MMP9 (92 kDa type IV collagenase) is also a product of some cancer cell lines (Okada et al, 1990(Okada et al, , 1992Shima et al, 1993), squamous cell carcinoma of the skin (Pyke et al, 1992) and prostate adenocarcinoma (Hamdy et al, 1994) but also localises to tumour-infiltrating mononuclear cells, especially macrophages (Naylor et al, 1994).…”

Expression and differential regulation of connective tissue growth factor in pancreatic cancer cells

“…SUIT-2 cell lines are clones derived from one primary tumor that di er in their spontaneous metastatic potential in nude mice. For example, the SUIT-2 clone 007 has been shown to have the highest and the SUIT-2 clone 028 the lowest metastatic potential after subcutaneous injection in nude mice (Taniguchi et al, 1992). Expression of CTGF and ®sp12 occurred independent of the metastatic potential of these cell lines (Figure 3).…”

Endostatin expression in a pancreatic cell line is modulated by a TNFα-dependent elastase

“…SUIT-2 is a human pancreatic tumour cell line derived from a liver metastasis and is known to display a highly metastatic phenotype with spontaneous metastasis to lung and regional lymph nodes from subcutaneous nude mouse xenografts (Taniguchi et al, 1992). BxPc-3 were derived from an adenocarcinoma of the body of the pancreas and are moderately differentiated (Tan et al, 1986).…”