Correlation between spontaneous metastatic potential, platelet-aggregating activity of cell surface extracts, and cell surface sialylation in 10 metastatic-variant derivatives of a rat renal sarcoma cell line.

Abstract

Several properties of 10 cell lines derived from the polyoma-induced PW20 Wistar-Furth rat renal sarcoma have been examined, including the ability of the tumor cells to metastasize spontaneously from subcutaneous sites in syngeneic hosts, the platelet-aggregating activity of material extracted by urea from the surface of cultured cells, the sialic acid content of the platelet-aggregating material, and the degree of sialylation of cell surface glycoconjugates in cultured cells. A correlation has been observed among all of these parameters. The results suggest a possible link between the degree of cell surface sialylation of tumor cells, their ability to aggregate platelets, and their ability to metastasize. (7,8).In this paper we report the platelet-aggregating activity of the PAM extracted from cells of 10 derivatives of the polyoma-induced PW20 Wistar-Furth rat renal sarcoma cell line, which vary in their ability to metastasize spontaneously from subcutaneous sites of tumor growth (9). The PAM's activity was found to correlate positively with the propensity of the cells to metastasize. In addition, the PAM's activity correlates with the sialic acid content of the PAM and with the degree of cell surface sialylation of the cultured tumor cells. METHODSCell Lines. The PW20 family of tumor cell lines (Table 1) was derived from a culture of the polyoma virus-induced PW20 Wistar-Furth rat renal sarcoma (obtained from H. 0. Sjbgren, Univ. of Lund, Sweden) by varying conditions of passage in tissue culture and passage through syngeneic animals (9). The RO(L) and RO(H) cell lines represent low and high tissue culture passages, respectively, of the original cell culture. The R1 cell line was obtained by reestablishing a subcutaneous RO(L) tumor in tissue culture and maintaining the culture for a large number of passages. The R2 cell line was similarly produced by reculturing a subcutaneous R1 tumor and maintaining the resultant culture for a prolonged period. The RO(L)R1, RO(H)R1, R2R1, and R2R2 cell lines were produced by reculturing subcutaneous RO(L), RO(H), R2, and R2R1 tumors, respectively; the R2L1 and R2N1 cell lines were obtained by culturing explanted lung (L) and lymph node (N) metastases that developed from subcutaneous R2 tumors. These last-named lines were all tested at low passage numbers. In vttro assays were performed with cells within several passages of those listed in The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.

Antimetastatic effects of synthetic polypeptides containing repeated structures of the cell adhesive Arg-Gly-Asp (RGD) and Tyr-Ile-Gly-Ser-Arg (YIGSR) sequences

“…Once in circulation, metastatic tumour cells encounter various host cells or components (Fidler, 1984 (Pearlstein et al, 1980). The adhesive interaction between tumour cells and/or platelets may form homotypic or heterotypic cell clumps and aggregations which can subsequently be arrested and extravasated (Terranova et al, 1982(Terranova et al, , 1984.…”

“…Many different types of tumour cell have been seen to elicit the activation and aggregation of platelets in vitro (Gasic et al, 1973;Pearlstein et al, 1980). These properties have been correlated with the metastatic potential of tumour cells.…”

A comparative study of some growth characteristics and cell-surface properties of neoplastic cells

“…Sialic acids which are responsible for an important part of the surface charge have been involved in metastasis formation. A significant correlation was found between cell surface sialylation and metastatic potential (Pearlstein et al, 1980). However, the exact mechanism by which sialic acids could have a determining role in metastasis is not known.…”

“…The activity of enzymes near to cell surfaces regulates cell detachment (Weiss, 1979 (Wallach, 1975). In these cell interactions, sialic acids appear to play a major role in determining the essential properties of the cell surface (Jeanloz & Codington, 1976;Pearlstein et al, 1980).…”

Increased sialyl Lewis A expression and fucosyltransferase activity with acquisition of a high metastatic capacity in a colon cancer cell line

“…These carbohydrate antigens have been used as tumour markers for preoperative diagnosis of colon cancer. Carbohydrate antigens may affect cellular adhesiveness (Irimura et al, 1981; Dennis et al, 1982), immunogenicity, other immune recognition mechanisms (Gendler et al, 1988), induction of platelet aggregation (Pearlstein et al, 1980;Kjima-Suda et al, 1986), invasive characteristics (Bolscher et al, 1980) and probably other yet undescribed cellular behaviours that may affect the metastatic potential of tumour cells.…”