Correlation between yield and biochemical parameters in the mulberry silkworm,Bombyx mori L

Chatterjee, Subhrajyoti; Rao, C. G. P.; Chatterjee, Gaurab; Ashwath, S. K.; Patnaik, Anjana

doi:10.1007/bf01184928

Cited by 33 publications

(21 citation statements)

References 14 publications

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“…Amylase activity was assayed following the method of Chatterjee et al (1993) by incubating 10 µl of digestive juice in 0.2% starch as the substrate for 30 min at 37° C, and the reaction was stopped using 1% dinitrosalicylic acid followed by heating in boiling water for 5 min. Amylase assay was carried out in 5 replications for each B. mori breed.…”

Section: Methodsmentioning

confidence: 99%

Identification of RAPD Markers Linked to Digestive Amylase Genes using Near Isogenic Lines of the Silkworm,Bombyx mori

Ashwath

Sreekumar

Toms

et al. 2010

Journal of Insect Science

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Digestive amylase has been identified as a useful marker for breeding in the silkwrom, Bombyx mori L (Lepidoptera: Bombycidae), due to its wide genetic divergence, its role in better digestibility and robustness. The low yielding indigenous B. mori breeds of tropics like India are characterized by high activity amylase genes controlled by Amy div or dv alleles, while the high yielding breeds of temperate origin are endowed with ‘null’ type (Amy dn) with low activity. For improving the digestibility and survival of temperate breeds of Japanese origin, Near Isogenic Lines (NILs) were developed introgressing the Amy div and dv alleles from the Donor Parents (DPs) into the genetic background of the Recurrent Parents (RPs) with ‘null’ type of amylase, which showed significant improvement in viability of the NILs. With the objective to know whether the amylase gene itself may confer higher survival by improving digestibility or some other closely linked genes flanking the amylase locus is responsible for better viability of the NILs, RAPD profiles among six B. mori breeds comprising of the DPs, RPs, and NILs developed through introgression of Amy div or dv alleles were analysed using 27 sets of RAPD primers. Out of the 27 primers, six (OPA01, OPA06, OPA09, OPA15, OPAH03, and OPAH05) showed RAPD products linked to the amylase genes of the DPs introgressed in the NILs, which were absent in their respective RPs. Three amplicons of 1584 bp, 1904 bp, and 1232 bp were specific to Amy div allele and one amplified product of 1776 bp was found to be linked with the Amy dv allele. Interestingly, two PCR products of 2628 and 1375 bp were associated with both Amy div and dv alleles. The results are discussed in light of further characterization of these amplified products leading to identification of DNA sequences that may be responsible for better digestibility and higher survival in B. mori.

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Section: Methodsmentioning

confidence: 99%

Identification of RAPD Markers Linked to Digestive Amylase Genes using Near Isogenic Lines of the Silkworm,Bombyx mori

Ashwath

Sreekumar

Toms

et al. 2010

Journal of Insect Science

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“…In insects, critical larval weight together with larval duration accomplishes endocrine—mediated metamorphic processes (Pradeep et al 2000; Truman 2005) but the process is under genetic control (Dubrovskaya et al 2004). Correlation between quantitative traits and biochemical parameters had reported earlier in B. mori (Shibukawa et al 1986; Chatterjee et al 1993). Significant correlation among biomass traits reflects interrelation among physiologically important processes.…”

Section: Discussionmentioning

confidence: 52%

Molecular Markers for Biomass Traits: Association, Interaction and Genetic Divergence in SilkwormBombyx mori

2007

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Improvement of high yielding, disease resistant silkworm strains became imminent to increase production of silk, which is a major revenue earner for sericulturists. Since environment interacts with phenotype, conventional breeding did not result in commendable yield improvement in synthetic strains of silkworm, Bombyx mori. Identification of DNA markers associated with different economically important biomass traits and its introgression could assist molecular breeding and expression of stabilized high yielding characters, but genetic basis of most quantitative traits in silkworm is poorly understood due to its polygenic control. Correlation analysis (R = 0.9) revealed significant interrelation among biomass traits viz., larval duration (TLD), larval weight (LWT), cocoon weight (CWT), shell weight (SWT), shell ratio (SR) and floss content. PCR using inter simple sequence repeat (ISSR) primers revealed 92% polymorphism among 14 tropical and temperate strains of B. mori, with average diversity index of 0.747. Stepwise multiple regression analysis (MRA) selected 35 ISSR markers positively or negatively correlated with different biomass traits, illustrated polygenic control. ISSR marker 830.81050bp was significantly associated with LWT, CWT, SWT, SR and floss content, indicated its pleiotropic role. Two ISSR markers, 835.51950bp and 825.9710bp showed significant association with floss content and TLD. These markers were segregated in F2 generation and Chi-square test confirmed (χ2 = ~45; P < 0.05) its genetic contribution to the associated biomass traits. Strains, with both positively and negatively correlated markers, had intermediate mean value for biomass traits (eg. SWT = 0.17 ± 0.014 g in GNM and Moria) indicated interaction of loci in natural populations. Low yielding Indian strains grouped together by Hierarchical clustering. Chinese and Japanese strains were distributed in the periphery of ALSCAL matrix indicated convergence of genetic characters in Indian strains. Average genetic distance between Chinese strains and Indian strains (0.193) significantly (P < 0.01) varied from that between Chinese and Japanese strains. Interaction of loci and allelic substitutions induced phenotypic plasticity in temperate B. mori populations on tropic adaptation in India. These outcomes show possibility to combine favorable alleles at different QTL to increase larval, cocoon and shell weight.

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“…Also in literature it is well known that the majority of the economically important genetic traits of silkworms are qualitative in nature and that phenotypic expression is greatly influenced by environmental factors such as temperature, relative humidity, light, and nutrition (Chatterjee et al, 1993, Thiagarajan et al, 1993Ramesha et al, 2009). …”

Section: Introductionmentioning

confidence: 99%

Comparative Study of Biological Characters of Silkworm Eggs

Pașca¹,

Furdui²,

Mărghitaș³

et al. 2015

BUASVMCN-ASB

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The silkworm Bombyx mori L. is a domesticated insect considered as a reference in several domains (Mauchamp et al., 2008). The theoretical and practical importance of the silkworms are shown in many studies (Nagaraju et al., 1996;Kipriotis et al., 2000;Sudhakara et al., 2003;Vassileva et al., 2004;Petkov et al., 1998;Matei et al., 2002;. This study was conducted to compare four monovoltine breeds of Romanian silkworm, hatching in specific conditions, in different years (2001 and 2008), to see if there is a major difference between the biological characters of eggs belonging to those breeds in time. For both 2001 and 2008 years, four breeds AC29, AC, B1 and AB of Bombyx mori (eggs) were obtained from SC SERICAROM (Bucharest, Romania) and the same microclimate conditions were assuring. In total, per data set of each year were 16 variants because each of the breed was hatch in four variants, for more complete results of analyzed samples. The evaluation was made on the base of indices used for classified biological characters of silkworm eggs, comparative data shows that, between the same breeds hatching in different years, there is no significant difference. According to biological characteristics, the study allowed us to make a classification of the analyzed breeds. In the present research, we demonstrated that biological characters of silkworm eggs were well preserved and there are no significant variations between both set of data taken in study.

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Correlation between yield and biochemical parameters in the mulberry silkworm,Bombyx mori L

Cited by 33 publications

References 14 publications

Identification of RAPD Markers Linked to Digestive Amylase Genes using Near Isogenic Lines of the Silkworm,Bombyx mori

Identification of RAPD Markers Linked to Digestive Amylase Genes using Near Isogenic Lines of the Silkworm,Bombyx mori

Molecular Markers for Biomass Traits: Association, Interaction and Genetic Divergence in SilkwormBombyx mori

Comparative Study of Biological Characters of Silkworm Eggs

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