Correlation of aberrant proliferation with T‐cell growth factor in adult T‐Cell leukemia cells

Abstract

SUMMARYPeripheral blood lymphocytes from seven patients with adult T-cell leukemia (ATL) were found to lack PHA-responsiveness. However, in most of the cases, minute but distinct proliferation could be induced and maintained by human spleen cell conditioned medium containing PHA or by a combination of PHA and conditioned medium ofgibbon cell line, MLA-144 (MLA-144 CM). These results indicate that the lack of response to mitogens of ATL cells might be attributed not only to the failure ofthese cells to produce T-cell growth factor (TCGF) upon activation, but also to their poor responsiveness to TCGF. Furthermore, a direct proliferative response to mitogen-free MLA-144 CM was shown in two out of seven patients; these two patients experienced rapidly progressive clinical courses. This observation raises the possibility that TCGF promotes the growth of ATL cells in vivo, and is related to the clinical course of the disease.KEY WORDS Adult T-cell leukemia Mitogen T-cell growth factor lnterleukin 2

On the Pinning of Downstream Meniscus for Slot Die Coating

“…The pinning of coating lines with special arrangements of the coating dies has appeared in several patents (O'Brien20 and Maier and Brown21). Tsuda et al22 proposed that the most effective way to pin the downstream meniscus to the coating die corner is to increase the static contact angle of the coating liquid on the die shoulder.…”