Correlation of apolipoprotein M with leptin and cholesterol in normal and obese subjects

Xu, N.; Nilsson‐Ehle, Peter; Åhrén, Bo

doi:10.1016/j.jnutbio.2004.03.001

Cited by 42 publications

(22 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In view of the report of a strong positive correlation of apoM and leptin in normal and obese subjects (29) and the well-established correlation between leptin and amount of body fat (30)(31)(32), the absence of any statistically significant correlation between apoM and body mass index in this study is somewhat surprising. Additionally, in the earlier study (27), a negative relationship with total cholesterol was found (r 5 20.41), a result in stark contrast to the positive relationship found in the present study.…”

Section: Discussioncontrasting

confidence: 68%

An ELISA for apolipoprotein M reveals a strong correlation to total cholesterol in human plasma

Axler

Ahnström

Dahlbäck

2007

Journal of Lipid Research

103

View full text Add to dashboard Cite

Apolipoprotein M (apoM) is a 188 amino acid, 25 kDa protein belonging to the lipocalin protein superfamily. Although predominantly associated with high density lipoprotein, apoM is found in all major lipoprotein classes. To facilitate clinical studies of apoM, we have developed a sandwich ELISA for the measurement of apoM in human plasma. This method has been used to investigate normal apoM variation and to establish reference values for healthy individuals through the measurement of 598 samples from the Nordic Reference Interval Project Bio-bank and Database (NOBIDA) biobank. For women 18-49 years old, the reference interval for apoM was 0.58-1.18 mmol/l, whereas for women 501 years and for men, the reference range was 0.61-1.30 mmol/l. Correlation studies of apoM with 26 common clinical chemical analytes from the NOBIDA database revealed a marked positive correlation with plasma total cholesterol (r 5 0.52) and LDL and HDL cholesterol (r 5 0.43 and 0.36, respectively). There was no statistically significant correlation with HDL/total cholesterol ratio or body mass index. In conclusion, a sandwich ELISA for the measurement of apoM in human plasma shows that apoM concentration is strongly correlated to total cholesterol in healthy individuals.-Axler, O., J. Ahnström, and B. Dahlbäck. An ELISA for apolipoprotein M reveals a strong correlation to total cholesterol in human plasma.

show abstract

Section: Discussioncontrasting

confidence: 68%

An ELISA for apolipoprotein M reveals a strong correlation to total cholesterol in human plasma

Axler

Ahnström

Dahlbäck

2007

Journal of Lipid Research

103

View full text Add to dashboard Cite

show abstract

“…We have also reported that apoM concentration in plasma was positively correlated to leptin level in obese subjects [6]. In the present study, we further examined the effects of leptin on apoM synthesis in HepG2 Leptin is a cytokine-like peptide secreted by adipose tissue, known to regulate hepatic lipid and lipoprotein metabolism [12][13][14][15].…”

Section: Discussionmentioning

confidence: 77%

“…In the leptin deficient (ob/ob) mouse and leptin receptor deficient (db/db) mouse the apoM mRNA levels and plasma apoM concentrations were greatly reduced when compared to the normal mouse. Furthermore, it is also reported that plasma apoM concentration is positively correlated to leptin level in obese subjects [6].…”

Section: Introductionmentioning

confidence: 94%

Leptin inhibits apolipoprotein M transcription and secretion in human hepatoma cell line, HepG2 cells

Luo

Hurtig

Zhang

et al. 2005

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

Self Cite

View full text Add to dashboard Cite

“…Simvastatin has been shown to reduce total mortality rates in patients with coronary heart disease [7]. Previous studies have shown that the plasma apoM concentration is positively correlated with leptin levels and negatively correlated with total cholesterol in normal and obese subjects [8]. ApoM gene expression could be directly regulated by transcription factors including transforming growth factor (TGF)-β, hepatic nuclear factor (HNF)-1α, liver receptor homolog (LRH)-1 and forkhead box A2 (Foxa2), all these could regulate hepatic lipid metabolism [9-12].…”

Section: Introductionmentioning

confidence: 99%

Effects of simvastatin on apolipoprotein M in vivo and in vitro

Zhang¹,

Mao²,

Luo³

et al. 2011

Lipids Health Dis

Self Cite

View full text Add to dashboard Cite

ObjectiveTo investigate effects of lipid lowering drug, simvastatin, on apolipoprotein M expression in the hyperlipidemic mice and in hepatic cell line, HepG2 cells.MethodsSwiss male mice were randomly divided into the high fat group and control group, and were intragastrically fed with 0.9% saline (control group) or lipid emulsion (high fat group) at the daily dosage of 15 ml/kg body weight, respectively. After 8 weeks feeding, the hyperlipidemic model was successfully induced and these hyperlipidemic mice were then randomly divided into three experimental groups: vehicle control group, high-dose simvastatin-treated group (100 mg/kg body weight), and low-dose simvastatin-treated group (10 mg/kg body weight). Mice were dosed daily for 6 weeks of simvastatin before mice were sacrificed for determining serum lipid profile and apoM protein levels that was determined by using dot blotting analysis. Effects of simvastatin on apoM mRNA expression in the HepG2 cells were determined by real-time RT-PCR.ResultsComparing to high fat model mice without simvastatin treatment, 100 mg/kg simvastatin could significantly increase serum total cholesterol (P < 0.05). Serum apoM levels, in all mice, were significantly lower in the mice at the age of 26 weeks than the mice at 12 weeks old (P < 0.05), which indicated that serum apoM levels were significantly correlated to the mice age. It demonstrated also that treatment of simvastatin did not influence serum apoM levels in these mouse model, although serum apoM levels were increased by about 13% in the 10 mg/kg simvastatin group than in the vehicle control group without simvastatin. In HepG2 cell cultures, simvastatin could significantly decrease apoM mRNA levels with dose- and time-dependent manners. At 10 μM simvastatin treatment, apoM mRNA decreased by 52% compared to the controls.ConclusionThe present study suggested that simvastatin, in vivo, had no effect on apoM levels in the hyperlipidemic mouse model. ApoM serum levels in mice were significantly correlated to the animal's age, whereas in cell cultures simvastatin does inhibit apoM expression in the HepG2 cells. The mechanism behind it is not known yet.

show abstract

Correlation of apolipoprotein M with leptin and cholesterol in normal and obese subjects

Cited by 42 publications

References 31 publications

An ELISA for apolipoprotein M reveals a strong correlation to total cholesterol in human plasma

An ELISA for apolipoprotein M reveals a strong correlation to total cholesterol in human plasma

Leptin inhibits apolipoprotein M transcription and secretion in human hepatoma cell line, HepG2 cells

Effects of simvastatin on apolipoprotein M in vivo and in vitro

Contact Info

Product

Resources

About