Correlation of change in phage type with pulsed field profile and 16S rrn profile in Salmonella enteritidis phage types 4, 7 and 9a

Abstract

SUMMARYUsing pulsed-field gel electrophoresis (PFGE) and 168 rRNA (rrn) analysis (ribotyping), the in vivo derivation of strains of Salmonella enteritidis PTs 9a and 7 from a strain of S. enteritidis PT 4 has been demonstrated. All strains were isolated from a single patient over a 6-week period. Further studies have demonstrated that in terms of pulsed-field profile and ribotype, the genotypes of the patient-derived strains differed from those of the reference strains of the respective phage types. It is concluded that when used in combination, these methods can provide evidence of phylogenetic relationships in apparently unrelated S. enteritidis phage types isolated during pathogenesis of disease.

Evaluation of a Salmonella Enteritidis vaccine and related ELISA for respective induction and assessment of acquired immunity to the vaccine and/or Echinacea purpurea in Awassi Ewes

“…The SE strain used in this new bacterin is of higher virulence due to its invasive nature, caused by the presence of a high size plasmid of 38 MDa, known to exist in highly virulent SE strains of phage type 4 [24].…”

Epidemiological analysis of strains of Salmonella enterica serotype Enteritidis from foodborne outbreaks occurring in Italy, 1980-1994

“…These include plasmid profiling [17, 181, IS200 typing [ 191, pulsed-field gel electrophoresis [20,21] and ribotyping [ 18,. Nevertheless, application of these techniques to strains belonging to PT4 or PT8, which have caused the majority of the outbreaks reported in Europe and in the United States, respectively, exhibited insufficient discriminatory power in many instances and consequently often proved to be unsuitable as effective epidemiological tools.…”

Salmonella

“…Typhi (Thong et al 1994), Newport (Ward et al 2002), Stanley (Kirk et al 2004) and phage type, e.g. Enteritidis PT 4 (Powell et al 1994(Powell et al , 1995, S. Typhimurium DT 104 (Lawson et al 2004). Since the mid-1990s PFGE has been used for characterization of a strain of Agona PT 15 responsible for an international outbreak of salmonellosis in at least four countries (Threlfall et al 1996b) and for the discrimination of a strain of S. Newport responsible for an outbreak of salmonellosis in England and Wales in 2001 associated with the consumption of contaminated salad vegetables originating in Spain (Ward et al 2002).…”