Correlation of Chlorophyll Meter Readings with Gas exchange and Chlorophyll Fluorescence in Flag Leaves of Rice (Oryza sativaL.) Plants

Abstract

fax +81-92-642-2833). Abbreviations : Chl, chlorophyll; F o , initial Chl fl uorescence of a dark-adapted leaf; F m , the maximum Chl fl uorescence of a darkadapted leaf; F v , variable Chl fl uorescence; N, nitrogen; NPT, new plant type; P G , gross photosynthetic rate; PPFD, photosynthetic photon fl ux density; PSII, photosystem II; Rubisco, ribulose-1,5-bisphosphate carboxylase/oxygenase. Abstract : The objective of this study was to establish the correlation of the chlorophyll meter (SPAD) readings with the contents of chlorophyll (Chl) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), the gross photosynthetic rate (P G ), and the maximum quantum yield of photosystem II (PSII) (F v /F m ) in fl ag leaves of rice (Oryza sativa L.) in ripening stage. The SPAD readings signifi cantly correlated with the Chl content, the Rubisco content, P G and F v /F m (R 2 = 0.848, 0.648, 0.671 and 0.712, respectively), which suggests that the SPAD meter has the potential to estimate the photosynthetic capacity of the fl ag leaves. However, both P G and F v /F m had a stronger relationship with the Rubisco content than the SPAD readings, indicating that the PSII photochemical and CO 2 assimilation capacities are strongly infl uenced by the Rubisco content. Therefore, accurate calibration would be indispensable to obtain the physiological information from the SPAD readings of fl ag leaves. Correlation of Chlorophyll Meter

A Quantitative Trait Locus for Chlorophyll Content and its Association with Leaf Photosynthesis in Rice

“…On the other hand, a digital chlorophyll meter (Soil and Plant Analyzer Development [SPAD] meter) provides a non-destructive method for estimating leaf chlorophyll content by measuring light absorption of specific spectral bands in living leaves (Watanabe et al 1980;Chubachi et al 1986). The methods for measurement of SPAD values are simple and quick, and close correlations between SPAD values and leaf photosynthesis values have been observed in rice (Huang and Peng 2004;Kato et al 2004;Kumagai et al 2009). Therefore, SPAD measurement may be a more appropriate method than destructive measurement of leaf chlorophyll content for use in genetic analysis of leaf photosynthesis.…”

Early-Season Soil Waterlogging and N Fertilizer Sources Impacts on Corn N Uptake and Apparent N Recovery Efficiency

“…On the 7th day of waterlogging, the CM readings were significantly lower in the 7-days waterlogged plots compared to non-waterlogged plots in all three years of this experiment ( Figure 4A-C). Previous studies have shown that CM readings were highly correlated with leaf chlorophyll and N content [38][39][40]. In waterlogged treatments, the lower CM readings might have resulted from a decrease in leaf chlorophyll content due to chlorophyll destruction mediated by superoxide radicals formed under waterlogging stress [41][42][43][44].…”

Relationship between photochemical efficiency (JIP-Test Parameters) and portable chlorophyll meter readings in papaya plants

“…In rice, Kumagai et al (2009) correlated PCM readings with some photosynthetic parameters, suggesting that the PCM readings are an indirect indication of the photosynthetic capacity in the flag leaves. In Amaranthus vlitus, there was a linear correlation between PCM readings and some physiological parameters of the leaves, such as photosynthesis, transpiration, and stomatal conductance.…”