Correlation of Circulating 17β-Oestradiol with Haemostatic Factors in Healthy Postmenopausal Women

Abstract

The aim of the study was to evaluate any correlation between the circulating oestrogenic hormone 17β-oestradiol and haemostatic factors in healthy postmenopausal women. In keeping with this objective, the correlations were evaluated irrespective of whether the source of the hormone was purely endogenous or exogenous as well. Accordingly, a univariate correlation adjusted for age, body mass index, and duration of menopause was determined in 42 healthy postmenopausal women aged 47–78 years, 19 of whom were self-reported users of hormone replacement therapy. The rest were self- reported never users. Serum 17β-oestradiol exhibited a direct correlation with endogenous thrombin potential extrinsic pathway (R = 0.42, p = 0.01) and prothrombin fragments 1 and 2 (R = 0.37, p = 0.03) and an inverse correlation with antithrombin III (R = –0.36, p = 0.03) and α₂-antiplasmin (R = –0.45, p = 0.005). The observations suggest an association of this hormone with net thrombin generation on the one hand and improved fibrinolysis on the other.

α2-Antiplasmin is involved in bone loss induced by ovariectomy in mice

“…Genome-wide screens demonstrate the existence of high-affinity estrogen receptor-binding sites in mouse liver for α 2 -AP gene [30,31]. Our present data that plasma α 2 -AP levels were significantly elevated in mice with OVX, compared to control mice, were compatible with those previous data [28,29]. Moreover, estrogen suppressed the expression of α 2 -AP in mouse hepatocytes.…”

“…Based on these results, we speculate that circulating α 2 -AP is produced in the liver and kidney, but is not locally produced in the bone. Cleuren et al reported that administration of estrogen significantly reduced plasma α 2 -AP in mice [28] and Mukherjee et al found a negative correlation between blood α 2 -AP and estradiol levels in patients undergoing hormone replacement therapy [29]. Genome-wide screens demonstrate the existence of high-affinity estrogen receptor-binding sites in mouse liver for α 2 -AP gene [30,31].…”

Effect of Hormone Replacement Therapy in Normalizing Age Related Neuronal Markers in Different Age Groups of Naturally Menopausal Rats

“…It is known that during aging the number of neurons is reduced and the protein synthesis and turnover rate is decreased (Zs-Nagy 1985). Mukherjee et al (1999) who showed that the total protein synthesis is significantly higher in adult males than females and decreases in old males but not in females, also reported that estradiol treatment enhances the synthesis of proteins remarkably in both sexes, 9.7-fold in adults, 4.1-fold in old males, 4.0-fold in adults and 3.4-fold in old females. On the other hand, the intracellular dry mass content increases i.e.…”

One Year Follow-Up of Hormone Replacement Therapy with Percutaneous Estradiol and Low-Dose Vaginal Natural Progesterone in Women with Mild to Moderate Hypertension

“…The effect of HRT on coagulation factors is also a matter of controversy. An inverse correlation between estradiol and antithrombin III levels has been observed in healthy postmenopausal women pooled without distinction between HRT users and nonusers (Mukherjee et al, 1999). Controlled prospective studies have suggested that HRT is associated with decreased levels of coagulation inhibitors, although a net activation of coagulation is not observed (Hoibraaten et al, 2000;van Baal et al, 2000).…”