Correlation of clinical, cytological and histological findings in oral squamous cell carcinomas

Sousa, Michele Cardoso; Alves, Mônica Ghislaine Oliveira; Souza, Luciano Albino; Brandão, Adriana Aigotti Haberbeck; Almeida, Janete Días; Cabral, Luiz Antônio Guimarães

doi:10.3892/ol.2014.2212

Cited by 8 publications

(7 citation statements)

References 26 publications

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“…As a result of cytopathological grading, the real picture came into existence and so an increasing trend was observed from precancerous lesions to malignant cases due to shifting of numbers. The cytological findings of Sousa et al (2014) have demonstrated that the 86.79% of the cases were found to have malignant potential [46]. Thus, the present finding corroborates the earlier reports of Mc Kinley and Sousa et al Francois et al (1997) have reported that chromatins were very finely granular and almost invisible in normal oral squamous cells, while these chromatins form well defined irregular clumps with variable size, shapes and sharp pointed projections in neoplastic cells.…”

Section: Discussionsupporting

confidence: 82%

Nuclear pleomorphism-based cytopathological grading in human oral neoplasm

Mohanta¹,

Prafulla²

2017

Russ. Open Med. J.

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Objective -The objective of the present study is record the nuclear pleomorphism in various stages of oral carcinogenesis and to analyse their utility in cytopathological grading for early detection of human oral cancer Material and Methods -In this hospital based case-control study, oral site, age-group and sex-matched 272 subjects (136 cases and 136 normal healthy individuals) were included. Scraped exfoliated cytosmears were collected from the affected oral site of the subjects and smearing was done in the pre-cleaned-coded glass-slides. Two such slides were prepared from each subject. The cytosmears were immediately fixed in aceto-alcohol (1 part of glacial acetic acid: 3 part of absolute ethyl alcohol) fixative. One set of the slide was stained with Papanicolaou's stain and the other set was counter-stained with Giemsa's Solution for cytopathological analysis. Test of proportion (ztest) was followed and the critical ratio (z-value) was calculated for the test of significance. Results -Nuclear pleomorphism in the form of round, oval, spindle, elongated fiber as well as irregular shapes were mostly observed in oral squamous cells during different stages of carcinogenesis. Appearance of such nuclear pleomorphism in human oral neoplasm may be considered as a sign of cellular alternation in general and index of oral carcinogenesis in particular. In the present study, the frank malignant cases mimic to be either premalignant lesions or benign/carcinoma in situ were detected on the basis of nuclear pleomorphismbased cytopathological grading and so an increasing trend was observed from precancerous lesions to malignant cases due to shifting of numbers. Diagnostic tests also indicated that the Sensitivity was calculated to be 83.5%, Specificity was 100%, positive predictive value (PPV) was 100%, negative predictive value (NPV) was 30% and the accuracy was found to be 84.6%. Therefore, the nuclear pleomorphismbased cytopathological grading system makes itself an ideal screening test for early detection of human oral cancer. Conclusion -Pattern of nuclear pleomorphism corresponding to various cytological atypias is a common feature observed during different stages of oral carcinogenesis and thus, it has a practical implication in grading and early detection of oral cancer.

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Section: Discussionsupporting

confidence: 82%

Nuclear pleomorphism-based cytopathological grading in human oral neoplasm

Mohanta¹,

Prafulla²

2017

Russ. Open Med. J.

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“…Exfoliative cytology is a reliable tool for assessing malignant change in various organs [ 1 ]. Cytology has been also applied to the diagnosis of oral lesions [ 2 – 4 ]. Accurate cytological diagnosis of oral lesions, especially in distinguishing benign lesions from malignant ones, is essential for treatment as well as for clinical and epidemiological research including the study of prognosis [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Diagnostic accuracy of oral cancer cytology in a pilot study

et al. 2017

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BackgroundRecently, cytology has been applied to the diagnosis of oral lesions. We aimed to explore the diagnostic accuracy of oral cytology based on the histological diagnosis.MethodsHistological diagnoses of 327 cases were classified as Negative, Borderline lesion –, Borderline lesion +, oral intraepithelial neoplasia/carcinoma in situ (OIN/CIS), or Positive. Cytological diagnoses were classified as NILM (negative for intraepithelial lesion or malignancy), LSIL (low-grade squamous intraepithelial lesion), HSIL (high-grade squamous intraepithelial lesion), or SCC (squamous cell carcinoma). The cytology slides were evaluated by 10 raters and the results were compared with the histology results.ResultsIn 142 cases that were histologically negative, the number of NILM, LSIL, HSIL, and SCC and other malignancy was 77 (54.2%), 47 (34.3%), 8 (5.6%), and 10 (7.0%), respectively. Among 32 cases of Borderline lesion –, the number of NILM, LSIL, HSIL, and SCC and other malignancy was 11 (34.3%), 11 (34.3%), 9 (28.1%), and 1 (3.1%), respectively. Also, in 4 cases of Borderline lesion +, the number of NILM, LSIL, HSIL, and SCC and other malignancy was 2 (50.0%), 0 (0.0%), 0 (0.0%), 2 (50.0%), respectively. Among 12 cases of OIN/CIS, the number of NILM, LSIL, HSIL, and SCC and other malignancy was 1 (8.3%), 2 (16.7%), 4 (33.3%), and 5 cases (41.7%), respectively. Among 137 cases with a histological diagnosis of Positive, the number of NILM, LSIL, HSIL, and SCC and other malignancy was 7 (5.1%), 22 (16.1%), 19 (13.9%), and 89 (65.0%), respectively. Sensitivity, specificity, and positive predictive and negative predictive values were 93.5, 50.6, 62.4, and 89.8%, respectively, when the cytological diagnosis of Negative was assumed to be NILM; they were 77.8, 83.9, 81.0 and 81.1%, respectively, if the cytological diagnosis of Negative was assumed to be NILM and LSIL. The number of false-positive and false-negative diagnosis affected cases with LSIL and HSIL may indicate the difficulty in the cytological diagnosis of borderline lesions. While the negative predictive value was relatively high (89.8%) when cytological Negative was assumed to be NILM only.ConclusionHistopathological examination should be recommended in cases with cytological diagnoses of LSIL, HSIL, and SCC.

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“…Relationships between histological characteristics and SCC progression have been reported in various organs, including OSCC (2,3). Four grades of mode of invasion were proposed by Jakobsson for the evaluation of cell invasiveness and proliferation of SCC in the larynx (4): grade 1 has a well-defined borderline; grade 2 has a lessmarked borderline; grade 3 has groups of cells and no distinct borderline; and grade 4 has diffuse growth.…”

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confidence: 99%