Correlation of collagenase secretion with metastatic-colonization potential in naturally occurring murine mammary tumours

Tarin, David; Hoyt, B J; Evans, D J

doi:10.1038/bjc.1982.192

Cited by 50 publications

(15 citation statements)

References 19 publications

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“…This model provided a quantitative assay of tumor cell invasion and demonstrated that natural collagenase inhibitors such as cartilage extract or the purified tissue inhibitor of metalloproteinases (TIMP) both prevented tumor cell invasion of the amnion matrix (23). Additional evidence that collagenolysis facilitates tumor cell invasion was provided by the report of a correlation between collagenase secretion and the metastatic colonization potential in naturally occurring murine mammary tumors (26). Thus immunolocalisation data and in vitro biochemical studies all support the hypothesis that collagen-degrading enzymes play a crucial role in the invasion of connective tissues.…”

Section: Extracellular Enzymesmentioning

confidence: 51%

“…Plasminogen activator is not unique to tumor cells and many normal cells express the enzyme. Its importance for tumor invasion is uncertain, especially as comparative studies of normal and tumor tissues revealed similar levels of plasminogen activator secretion (30), an observation also reported for murine mammary tumors which demonstrated both high and low pulmonary-colonizing potential (26). However, it has been suggested that the secretion of type IV collagenase concomitantly with plasminogen activator in several tumor cell cultures suggests this is a prerequisite for metastasis-latent collagenase being activated following the conversion of plasminogen to plasmin (31).…”

Section: Extracellular Enzymesmentioning

confidence: 98%

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Collagenolytic mechanisms in tumor cell invasion

Woolley¹

1984

Cancer Metast Rev

130

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Connective tissue stroma and basement membrane structures probably present natural barriers to the migration of tumor cells. It has therefore been proposed that collagenolytic enzymes are required to facilitate the spread and invasion of tumor cells into host tissues. The collagenases and cathepsin B-like enzymes are thought to be involved, but the cellular source of collagenolytic activity at the tumor: host interface or 'invasion zone' remains obscure in most cases. The 'invasion zone' of different tumors is very variable with regard to the type and numbers of host or tumor cells, as well as the type of collagenous matrix, and few generalities can be made. The existence within a tumor of specialised subpopulations of cells which have different metastatic potential has been postulated. As a consequence it seems plausible that the phenotypic expression of highly invasive or metastatic tumor cells should include the potential for generating collagenolytic activity. Immunolocalisation studies have demonstrated the production of type I and type IV collagenases at sites of tumor invasion, but it does not appear to be a continuous process and only a small proportion of tumor and/or host cells elaborate enzyme at any one moment. Collagenase production is invariably microenvironmental in nature and it seems likely that local host:tumor cell interactions are important in modulating collagenolysis. Macrophages and mast cells have been shown to stimulate collagenase expression by tumor and stromal cells in vitro, and it is proposed that these cells may assume a contributory role for the induction of collagenolytic activity in vivo. The collagenolytic mechanisms that operate at micro-foci of host:tumor junctions probably depend upon the type of collagen, the cellular composition and the extracellular ionic conditions of each invasion site. Either tumor or host cells may elaborate enzymes, this being dependent upon the type and/or tissue location of the invasive tumor.

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Section: Extracellular Enzymesmentioning

confidence: 51%

Section: Extracellular Enzymesmentioning

confidence: 98%

Collagenolytic mechanisms in tumor cell invasion

Woolley¹

1984

Cancer Metast Rev

130

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“…Cell suspensions were prepared as described previously (Tarin & Price, 1979 Tarin et al, 1982 for full explanation of the choice of these mathematical methods). In keeping with these decisions the median colonisation grade was used for categorisation of tumour colonisation potential, although results for each inoculated animal are also provided.…”

Section: Methodsmentioning

confidence: 99%

Effect of enzymic removal of cell surface constituents on metastatic colonisation potential of mouse mammary tumour cells

Sargent¹,

Price²,

Tarin³

1983

Br J Cancer

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“…This enzyme was also present in serum-free media from a human breast carcinoma cell line ALAB (21) and from a neoplastic epithelial cell line derived from spontaneous rat mammary adenocarcinoma (22). ColLagenase and plasminogen activator have been reported in naturally occurring mammary tumors of the mouse (23) and MCF-7 cells produce collagenolytic activities, able to degrade type I and type IV collagen (24). These findings are consistent with the idea that both interstitial collagenase and type IV collagenase are associated with invasiveness and microinvasive lesions of human breast carcinomas (18,24).…”

Section: Discussionmentioning

confidence: 93%

Biological significance of interstitial collagenase in DMBA-induced mammary tumors of the rat

Wirl¹

1984

Cancer Metast Rev

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In this review the production of interstitial collagenase in DMBA-induced mammary tumors of the rat has been examined. Cell sorting and cell cultures have given us the opportunity to relate the release of collagenase to a specific cell type. By means of FITC-fluorescence and monospecific antibodies (S. Sakamoto, Harvard University, Boston) it was further possible to localize collagenase in vitro and in vivo. The most outstanding characteristic is that collagenase is produced both by cuboidal, epithelial cell and by macrophages in vitro but not by myoepithelial-like cells. On the other hand, synthesis of collagenase in vivo was detected in some stromal cells, possibly macrophages, but not in neoplastic cuboidal cells. This observation has been related to the inability of cuboidal cells to interact with stromal, fibrillar collagen in vivo since tumor cells are arranged in glandular-like structures bordered by myoepithelial cells and a basement membrane. In vitro, fibrillar rat tail tendon collagen was found to be a potent stimulator of collagenase production by cuboidal cells. Collagenase stimulation by interstitial collagen therefore suggests a plausible mechanism for the degradation of collagen fibrils during local invasion by mammary tumor cells.

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Correlation of collagenase secretion with metastatic-colonization potential in naturally occurring murine mammary tumours

Cited by 50 publications

References 19 publications

Collagenolytic mechanisms in tumor cell invasion

Collagenolytic mechanisms in tumor cell invasion

Effect of enzymic removal of cell surface constituents on metastatic colonisation potential of mouse mammary tumour cells

Biological significance of interstitial collagenase in DMBA-induced mammary tumors of the rat

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