Correlation of enterohemorrhagic
            <i>Escherichia coli</i>
            O157 prevalence in feces, hides, and carcasses of beef cattle during processing

Elder, R. O.; Keen, James E.; Siragusa, Gregory R.; Barkocy-Gallagher, Genevieve A.; Koohmaraie, M.; Laegreid, William W.

doi:10.1073/pnas.97.7.2999

Cited by 675 publications

(543 citation statements)

References 22 publications

(23 reference statements)

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“…High prevalences of EHEC O157 in bovine faeces, 27.8% in United State [7], 19.7% in the United Kingdom [2] and 16.1% in the Czech Republic [4], have been shown previously. In Japan, it was previously reported that the isolation rate of EHEC O157 was 0.3% in faecal samples from slaughterhouse cattle in 1987 [11], 1.4% in faeces and 0.3% of dressed carcasses in 1996 [22], and 6.5% in faeces in 1999 [15].…”

mentioning

confidence: 94%

“…In particular, it is often isolated from faeces of cattle showing no clinical signs of disease. It was reported that the prevalence of EHEC O157 in cattle was 0.2-28% in the United States [7,8,23], 1.9% in Australia [5], and 4.2-20% in Europe [2,4,9,16]. Therefore, cattle can harbour the organisms in their faeces and are regarded as a natural reservoir and source of infection [3].…”

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confidence: 99%

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High Prevalence of Enterohemorrhagic Escherichia coli (EHEC) O157 from Cattle in Selected Regions of Japan

Ezawa

Gocho

Kawata

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. The prevalence of enterohemorrhagic Escherichia coli (EHEC) O157 was examined in bovine faeces. EHEC O157 was isolated from the faeces of 42 (13.0%) of 324 cattle. Of the 4 farms and the facilities tested, the 3 farms and the facilities wer e found positive for EHEC O157. The highest isolation rate among the farms was 33.7%. The prevalence of EHEC O157 in heifers was higher than that in calves and other cattle. No cattle positive for EHEC O157 showed any clinical signs except 2 calves with diarrhea in a veterinary hospital. Almost all isolates possessed the stx gene, and Stx-positive strains carrying both stx 1 and stx 2 genes were predominant. These results indicate that EHEC O157 are distributed in bovine faeces, and that dairy and beef farms in selected regions of Japan are heavily contaminated with the organisms. KEY WORDS: cattle, EHEC O157, prevalence.

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confidence: 94%

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confidence: 99%

High Prevalence of Enterohemorrhagic Escherichia coli (EHEC) O157 from Cattle in Selected Regions of Japan

Ezawa

Gocho

Kawata

et al. 2004

The Journal of Veterinary Medical Science

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“…Transmission of E. coli O157:H7 from cattle to humans occurs via foodborne and waterborne routes (3)(4)(5). Although E. coli O157:H7 strains occur in other animals, cattle have the highest known prevalence (1,2,6). Therefore, knowledge of the ecologic relationship between E. coli O157:H7 and its bovine reservoir host is critical to understanding the epidemiology of the disease and ultimately to the rational development of programs to eliminate the organism from cattle.…”

mentioning

confidence: 99%

“…C attle are a major reservoir for enterohemorrhagic Escherichia coli O157:H7 strains, which cause hemorrhagic colitis and hemolytic uremic syndrome (HUS) in humans (1)(2)(3). Transmission of E. coli O157:H7 from cattle to humans occurs via foodborne and waterborne routes (3)(4)(5).…”

mentioning

confidence: 99%

Cattle lack vascular receptors for Escherichia coli O157:H7 Shiga toxins

Pruimboom‐Brees

Morgan

Ackermann

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

175

143

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Escherichia coli O157:H7 causes Shiga toxin (Stx)-mediated vascular damage, resulting in hemorrhagic colitis and the hemolytic uremic syndrome in humans. These infections are often foodborne, and healthy carrier cattle are a major reservoir of E. coli O157:H7. We were interested in knowing why cattle are tolerant to infection with E. coli O157:H7. Cattle tissues were examined for the Stx receptor globotriaosylceramide (Gb3), for receptivity to Stx binding in vitro, and for susceptibility to the enterotoxic effects of Stx in vivo. TLC was used to detect Gb3 in tissues from a newborn calf. Gb3 was detected by TLC in kidney and brain, but not in the gastrointestinal tract. Immunohistochemistry was used to define binding of Stx1 and Stx2 overlaid onto sections from cattle tissues. Stx1 and Stx2 bound to selected tubules in the cortex of the kidney of both newborn calves (n ‫؍‬ 3) and adult cattle (n ‫؍‬ 3). Stx did not bind to blood vessels in any of the six gastrointestinal and five extraintestinal organs examined. The lack of Gb3 and of Stx receptivity in the gastrointestinal tract raised questions about the toxicity of Stx in bovine intestine. We found that neither viable E. coli O157:H7 nor Stx-containing bacterial extracts were enterotoxic (caused fluid accumulation) in ligated ileal loops in newborn calves. The lack of vascular receptors for Stx provides insight into why cattle are tolerant reservoir hosts for E. coli O157:H7.

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“…A presença da bactéria na faca dos manipuladores durante a evisceração mostra que este objeto não está sendo esterilizado de maneira corret, conforme designa a circular N o 175/2005, do MAPA, que preconiza a esterilização das facas por 20 segundos a 82ºC, no mínimo, após cada operação, pois durante a evisceração pode ocorrer uma contaminação da carcaça mais extensa por conteúdo gastrointestinal (Brasil, 2005). Elder et al (2000) também indicaram que o processo de evisceração é um ponto crítico de controle para a contaminação das carcaças, e estratégias de intervenção devem ser implementadas.…”

Section: Resultados E Discussão Ocorrência De Escherichia Coliunclassified

Ocorrência de Escherichia coli O157:H7 e O26 sorbitol negativas em matadouro frigorífico de bovino e suscetibilidade a antimicrobianos

et al. 2014

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Cattle beef could be a way of transmittingEscherichia coli O157: H7 and non-O157 to humans if they consume undercooked meat, being responsible for causing severe diseases such as hemolytic uremic syndrome. Bacterial resistance has become worrying concerning the efficacy in the treatment of diseases, and because of these aspects, the objective of this study was to verify the occurrence of E. coli O157: H7 and non-O157 in the stages of cattle slaughter and to evaluate the susceptibility of these bacteria against antimicrobial action. We collected from a cattle slaughterhouse 21 samples from manipulators' hands, 21 from knives and 300 from 50 animals in six points of the flowchart. The isolation was performed using the CT-SMAC agar and characterization of serotypes by PCR. There was higher occurrence of E. coli O157: H7 (12.0%) in animals and lower prevalence of E. coli O26 (8.0%) and O113 (2.0%). E. coli O26 was present in 9.52% of the knives. The presence of E. coli non-O157 sorbitol negative was an unexpected fact due to the method of isolation. All E. coli O157: H7 isolates were sensitive to tetracycline, cefepime, cefoxitin, ciprofloxacin and sulphazotrim, and 78.85% of them were resistant to cephalothin and 34.61% to ampicillin. All E. coli O26 were sensitive to cefepime, cefoxitin and sulphazotrim, and 88.23% were resistant to tetracycline and cephalothin and 82.35% to ampicillin. The antimicrobial multiresistance was observed in both serotypes. It should be, therefore, a criteria for using antimicrobial in treatments to avoid become a public health concern.

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Correlation of enterohemorrhagic Escherichia coli O157 prevalence in feces, hides, and carcasses of beef cattle during processing

Cited by 675 publications

References 22 publications

High Prevalence of Enterohemorrhagic Escherichia coli (EHEC) O157 from Cattle in Selected Regions of Japan

High Prevalence of Enterohemorrhagic Escherichia coli (EHEC) O157 from Cattle in Selected Regions of Japan

Cattle lack vascular receptors for Escherichia coli O157:H7 Shiga toxins

Ocorrência de Escherichia coli O157:H7 e O26 sorbitol negativas em matadouro frigorífico de bovino e suscetibilidade a antimicrobianos

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