Correlation of fetal DNA levels in maternal plasma with Doppler status in pathological pregnancies

Smid, Maddalena; Galbiati, Silvia; Lojacono, Andrea; Valsecchi, Luca; Platto, C.; Cavoretto, Paolo; Calza, Stefano; Ferrari, Augusto; Ferrari, Maurizio; Cremonesi, Laura

doi:10.1002/pd.1504

Cited by 46 publications

(27 citation statements)

References 27 publications

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“…Previous studies reported that plasma cfDNA levels are increased in pregnancies with clinically established PE [13,14,15,16], which has been attributed to placental ischemia, with release into the maternal circulation of necrotic or apoptotic syncytiotrophoblast fragments that contain fetal cfDNA [15,35]. Some contradictory evidence suggests that in cases of early-onset PE the increase in cfDNA precedes the clinical onset of the disease and may be apparent from the first trimester of pregnancy [35,36,37,38].…”

Section: Discussionmentioning

confidence: 99%

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Maternal Plasma Cell-Free Fetal and Maternal DNA at 11-13 Weeks' Gestation: Relation to Fetal and Maternal Characteristics and Pregnancy Outcomes

Poon

Musci²,

Song³

et al. 2013

Fetal Diagn Ther

200

101

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Objective: To examine the possible relationship between maternal and fetal characteristics and pregnancy outcomes on fetal and maternal cell-free (cf) DNA in maternal plasma at 11-13 weeks' gestation. Methods: cfDNA was extracted from maternal plasma of 1,949 singleton pregnancies and chromosome-selective sequencing was used to determine the proportion of cfDNA and total cfDNA counts which was of fetal and maternal origin. Multivariate regression analysis was used to determine whether specific maternal and fetal characteristics and pregnancy complications, such as preeclampsia (PE), early spontaneous preterm birth (SPB) and delivery of small for gestational age (SGA) neonates, were significant predictors of fetal and maternal cfDNA in maternal plasma. Results: The fetal and maternal cfDNA plasma concentration increased with serum pregnancy-associated plasma protein-A and free β-human chorionic gonadotropin level, was higher in women of Afro-Caribbean and East-Asian racial origin than in Caucasians, and lower in smokers, but it was not significantly altered in pregnancies complicated by PE, SPB or SGA. The fetal cfDNA level was inversely related to maternal weight and uterine artery pulsatility index, and maternal cfDNA increased with maternal weight. Conclusions: The fetal and maternal cfDNA level in maternal plasma is affected by maternal and fetal characteristics, but it is not altered in pregnancy complications.

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Section: Discussionmentioning

confidence: 99%

“…However, several studies in pregnancies with male fetuses in which maternal plasma cfDNA level was estimated by amplification loci on the Y-chromosome reported that there is a 2- to 5-fold increase in cfDNA in preeclampsia (PE), which is associated with impaired placentation reflected in low first-trimester serum PAPP-A [12,13,14,15,16]. …”

Section: Introductionmentioning

confidence: 99%

Maternal Plasma Cell-Free Fetal and Maternal DNA at 11-13 Weeks' Gestation: Relation to Fetal and Maternal Characteristics and Pregnancy Outcomes

Poon

Musci²,

Song³

et al. 2013

Fetal Diagn Ther

200

101

View full text Add to dashboard Cite

show abstract

“…Interestingly, amniotic fluid concentration of S100B was found to sensitively and specifically predict spontaneous intrauterine fetal death (Florio et al 2004). Although an early study conducted by Sekisawa et al found no significant difference in maternal plasma levels of cffDNA in nine women with IUGR compared with 20 gestation age-matched controls (Sekizawa et al 2003), more recent studies using larger cohorts found significant increases in women with IUGR (Smid et al 2006, Al Nakib et al 2009, Alberry et al 2009. Similarly, circulating mitochondrial DNA was also found to be increased in patients with IUGR (Colleoni et al 2010).…”

Section: Intrauterine Growth Restrictionmentioning

confidence: 99%

Sterile inflammation and pregnancy complications: a review

Nadeau-Vallée¹,

Obari²,

Palacios³

et al. 2016

Reproduction

219

161

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Inflammation is essential for successful embryo implantation, pregnancy maintenance and delivery. In the last decade, important advances have been made in regard to endogenous, and therefore non-infectious, initiators of inflammation, which can act through the same receptors as pathogens. These molecules are referred to as damage-associated molecular patterns (DAMPs), and their involvement in reproduction has only recently been unraveled. Even though inflammation is necessary for successful reproduction, untimely activation of inflammatory processes can have devastating effect on pregnancy outcomes. Many DAMPs, such as uric acid, high-mobility group box 1 (HMGB1), interleukin (IL)-1 and cell-free fetal DNA, have been associated with pregnancy complications, such as miscarriages, preeclampsia and preterm birth in preclinical models and in humans. However, the specific contribution of alarmins to these conditions is still under debate, as currently there is lack of information on their mechanism of action. In this review, we discuss the role of sterile inflammation in reproduction, including early implantation and pregnancy complications. Particularly, we focus on major alarmins vastly implicated in numerous sterile inflammatory processes, such as uric acid, HMGB1, IL-1α and cell-free DNA (especially that of fetal origin) while giving an overview of the potential role of other candidate alarmins.Reproduction (2016) 152 R277-R292

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“…However, due to the rare occurrence of these cells the much more likely source of cff DNA in maternal plasma is the placenta (Bianchi, 2004;Alberry et al, 2007). The kinetics of cff DNA accumulation in maternal plasma also suggest that the placenta is the likely source as cff DNA levels increase with gestational age (Birch et al, 2005;Galbiati et al, 2005;Smid et al, 2006). It is known from in vitro studies that hypoxia induces both apoptosis and necrosis in villous explants of the placenta, with an accompanying release of cellfree DNA.…”

Section: Origins Of Cell-free Plasma Dna and Identifying Biomarker Dmentioning

confidence: 99%

Noninvasive prenatal diagnosis of aneuploidy using cell‐free nucleic acids in maternal blood: promises and unanswered questions

2007

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The discovery of cell-free fetal (cff) DNA and RNA in the maternal circulation has driven developments in noninvasive prenatal diagnosis (NIPD) for the past decade. Detection of paternally derived alleles in cff DNA is becoming well established. Now much interest is focussing on NIPD of fetal chromosomal abnormalities, such as trisomy 21, which is a considerable challenge because this demands accurate quantitative measurements of the amounts of specific cff DNA or cff RNA sequences in maternal blood samples. Emerging strategies for distinguishing and quantifying the fetal nucleic acids in the maternal circulation promise continued development of the field, and pose a number of unanswered questions.

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Correlation of fetal DNA levels in maternal plasma with Doppler status in pathological pregnancies

Cited by 46 publications

References 27 publications

Maternal Plasma Cell-Free Fetal and Maternal DNA at 11-13 Weeks' Gestation: Relation to Fetal and Maternal Characteristics and Pregnancy Outcomes

Maternal Plasma Cell-Free Fetal and Maternal DNA at 11-13 Weeks' Gestation: Relation to Fetal and Maternal Characteristics and Pregnancy Outcomes

Sterile inflammation and pregnancy complications: a review

Noninvasive prenatal diagnosis of aneuploidy using cell‐free nucleic acids in maternal blood: promises and unanswered questions

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