Correlation of genetic variation among wild Trigonella foenum-graecum L. accessions with their antioxidant potential status

Abstract

ABSTRACT. In this study, we analyzed the correlation between genetic variation based on random amplified polymorphic DNA (RAPD), acid phosphatase, and glutamate-oxaloacetate transaminase isozymes, and amino acid composition with the antioxidant potential status of 7 wild Trigonella foenum-graecum L. accessions collected from diverse ecogeographical regions. RAPD revealed that 90 DNA products had highly polymorphism value (94.12%) based on band numbers, with sizes ranging from 50-2100 base pairs, and band intensity. Of 49 DNA polymorphic bands, 31 unique and 3 monomorphic bands were scored. Acid phosphatase and glutamate-oxaloacetate transaminase showed total polymorphism values of 90.00 and 93.75%, respectively, based on zymogram number, relative front (R f ), and optical intensity. Because isozymes are composed of amino acids, they were analyzed using highperformance liquid chromatography, which revealed the presences of 16 amino acids of variable content ranging from 13.21-15.35%, 9 of which are essential amino acids in humans. RAPD and isozymes showed similarly high estimates of genetic variability. Genetic relationships RAPD, isozymes, and antioxidant activity of fenugreek accessions revealed by unweighted pair group method with arithmetic mean clustering analysis based on data obtained from all primers of RAPD and each isozyme were very similar. The antioxidant potential based on free radical scavenging, 2, 2-diphenyl-1-picrylhydrazyl, b-carotenelinoleate, total phenolic, and flavonoid contents values were variable among accessions. We found that fenugreek is a valuable genetic resource with high antioxidant activity. Their genotypes, based on data and clustering of RAPD, isozymes, and variable amino acid contents, combined with their antioxidant potential statues are important in fenugreek breeding and improvement programs.

Comparative Evaluation of Hepatoprotective Activities of <i>Trigonella</i><i> foenum-graecum</i> (seeds), <i>Cymbopogon</i><i> proximus</i> and <i>Acacia nilotica</i> on a model of acute carbon tetrachloride hepatitis in rats

“…О наличии гепатопротекторной активности фитопрепаратов свидетельствуют многочисленные исследования как отечественные, так и зарубежных авторов [5,6]. По данным литературы исследуемые растворы трав обладают гепатотропными свойствами, но патофизиологические механизмы не изучены.…”

Genetic linkage between protein and DNA polymorphisms and antioxidant capacity of Cuminum cyminum L. accessions

“…DNA polymorphisms are produced by rearrangements or deletions at or between oligonucleotide primer binding sites within the genome (Williams et al, 1990) or from mutations in DNA sequences (either a change in the base sequence or in the number of copies of a specific DNA sequence), including substitution mutations (point mutations), rearrangements (insertions or deletions), or errors in the replication of tandemly repeated DNA (Haliem and Al-Huqail, 2014) due to environmental stress in different ecogeographical regions. Additionally, such changes in DNA configuration may lead to the loss or deletion of DNA bands (non-unique), gain (insertion) of amplified DNA bands (unique), changes in nitrogenous bases, or changes in DNA fragment size, and consequently, different length DNA fragments with high polymorphism that could be very valuable for DNA fingerprinting and the identification of genetic variations among different cumin germplasm accessions.…”

“…Studies have shown that the genetic diversity of cumin is high, possibly allowing it to adapt more easily to environmental variations (Bahraminejad et al, 2012). RAPD-PCR is an efficient method used for varietal identification, for the study of polymorphism, genetic biodiversity, and phylogenetic relationships among different plant species (Haliem and Al-Huqail, 2014;Ben Tamarzizt et al, 2015;Sharaf-Eldin et al, 2015), and especially among cumin accessions (Bahraminejad et al, 2012;Rostami-Ahmadvandi et al, 2013;Parashar et al, 2014;Choudhary et al, 2015).…”

The Promise of Molecular and Genomic Techniques for Biodiversity Research and DNA Barcoding of the Arabian Peninsula Flora

“…However, Z. coccineum demonstrated higher levels of genetic variation than the other species (Hammad and Qari, 2010). Similarly, Haliem and Al-Huqail (2014) studied the genetic variation in fenugreek ( Trigonella foenum-graecum ) ( n = 7) from Saudi Arabia and Yemen using RAPD, concluding that this species exhibited a high level of polymorphism (94.2%).…”