Correlation of hepatic metallothionein concentrations with acute cadmium toxicity in the mouse

Probst, Gregory S.; Bousquet, William F.; Miya, Tom S.

doi:10.1016/0041-008x(77)90177-6

Cited by 157 publications

(30 citation statements)

References 21 publications

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“…Metallothioneins have virtually no aromatic amino acids and a high cystein content (> 30 0/0); their role as protection agents is undisputed in principle (Webb, 1975;Probst et al, 1977).…”

Section: Discussionmentioning

confidence: 99%

“…In vertebrates, Cd is bound to mid molecular weight proteins, metallothioneins, which serve as protective proteins against acute toxicity and detrimental effects of "free" Cd ions on cytoplasmatic enzymes (Webb, 1975;Probst et al, 1977). Similarly, metallothioneins are responsible for primary accumulation of the metal in kidney and liver of the test animals (Tanaka et al, 1975;Overnell et al, 1977).…”

Section: Introductionmentioning

confidence: 99%

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Accumulation, loss and molecular distribution of cadmium inMytilus edulis

Scholz

1980

Helgolander Meeresunters

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In Mytilus edulis, accumulation and loss of Cd were analyzed under experimental conditions. Cd uptake by the whole soft body is linear, increasing significantly with increasing Cd concentrations in the uptake medium. Until 100 #g Cd 1-1, neither limitation of uptake nor any saturation process can be observed. Loss of Cd, measured after transfer of experimentally contaminated mussels to natural sea water, is exponential; biological half lives vary between 14 and 29 days. Gills are the primary sites of Cd uptake from the water, whereas in mid-gut gland, kidney, and mantle the uptake is retarded during the first few days. The mid-gut gland not only bears the main body load of Cd, but also shows the highest Cd concentrations. Gel chromatographic studies of midgut gland proteins reveal that Cd is eluated over the whole molecular weight range. Three metallothionein-like proteins with molecular weights of 6,600, 13,200, and 21,000 Dalton could be established. However, they cannot be taken as effective detoxification proteins, because more than 50 % of the accumulated metal is bound to high molecular weight proteins.

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“…Metallothioneins have virtually no aromatic amino acids and a high cystein content (> 30 0/0); their role as protection agents is undisputed in principle (Webb, 1975;Probst et al, 1977).…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Accumulation, loss and molecular distribution of cadmium inMytilus edulis

Scholz

1980

Helgolander Meeresunters

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“…Owing to these abilities, the protective role of MT against metal toxicity has been extensively investigated. For instance, pretreatment with Zn or low doses of Cd as a MT inducer results in reduced lethality after administration of high doses of Cd (Webb, 1972;Probst et al, 1977;Coogan et al, 1994). Additionally, we previously showed that multiple injections with Zn or Cd significantly increased MT levels compared to single injections with Zn or Cd Cherian, 1981, 1982).…”

Section: Introductionmentioning

confidence: 99%

Carbon tetrachloride-induced lethality in mouse is prevented by multiple pretreatment with zinc sulfate

et al. 2016

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-Carbon tetrachloride (CCl 4 ) is commonly used as a chemical inducer of experimental liver injury. Several compounds have been demonstrated to attenuate the hepatic damage caused by sublethal doses of CCl 4 . However, rescue from lethal toxicity of CCl 4 has not been reported. In the present study, we evaluated the protective effect of metallothionein (MT), an endogenous scavenger of free radicals, on CCl 4 -induced lethal toxicity of mice. To induce MT production in male ddY mice, we administered Zn (as ZnSO 4 ) at 50 mg/kg as a once-daily subcutaneous injection for 3 days prior to a single intraperitoneal administration of 4 g/kg CCl 4 . Animals were observed for mortality every 3 hr for 24 hr after CCl 4 injection. Liver damage was assessed by determining (in a subset of these mice) blood levels of alanine aminotransferase (ALT; a marker of liver injury) and liver histopathology at 6 hr after CCl 4 injection. Our results showed that three times pretreatment with Zn yielded > 40-fold induction of hepatic MT protein levels compared to control group. Zn pretreatment completely abolished the CCl 4 -induced mortality of mice. We also found that pretreatment of mice with Zn significantly decreased the ALT levels and reduced the histological liver damage as assessed at 6 hr post-CCl 4 . These findings suggest that prophylaxis with Zn protects mice from CCl 4 -induced acute hepatic toxicity and mortality, presumably by induction of radical-scavenging MT.

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“…The relationship between Cd intake and the loss of bone density has been investigated in detail in order to clarify the mechanisms underlying Itai-itai disease [14][15][16][17][18][19][20] . MT has been shown to play an important role in the detoxification of Cd due to binding with Cd through cysteine thiol groups.…”

Section: Discussionmentioning

confidence: 99%

Changes in Metallothionein Isoform Expression in the Bones of Ovariectomized Rats

Sogawa

Ikegame

Miyazaki

et al. 2016

J. Hard Tissue Biology.

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Metal-binding proteins, metallothioneins (MTs), may play important roles in bone metabolism. However, the contribution of MTs to bone metabolism remains obscure. In the present study, we investigated the expression of MT isoforms in bone cells and mRNA for MT isoforms in the tibiae following ovariectomy (OVX). The results obtained showed that MT-I/II and MT-III were expressed in osteoblasts, osteoclasts, and osteocytes 4 weeks after OVX. Peaks in the mRNA expression ratios (OVX/Sham) of MT-I/II and MT-III changed following OVX. The expression ratio of MT-I/II increased after 1 week, whereas that of MT-III increased 4 weeks after OVX. These results suggest that the contribution of MTs to bone metabolism may depend on the isoforms in the cell types and the stage after OVX.

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Correlation of hepatic metallothionein concentrations with acute cadmium toxicity in the mouse

Cited by 157 publications

References 21 publications

Accumulation, loss and molecular distribution of cadmium inMytilus edulis

Accumulation, loss and molecular distribution of cadmium inMytilus edulis

Carbon tetrachloride-induced lethality in mouse is prevented by multiple pretreatment with zinc sulfate

Changes in Metallothionein Isoform Expression in the Bones of Ovariectomized Rats

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