Correlation of
            <i>Campylobacter</i>
            Bacteriophage with Reduced Presence of Hosts in Broiler Chicken Ceca

Atterbury, Robert J.; Dillon, E.; Swift, Craig; Connerton, Phillippa L.; Frost, J. A.; Dodd, C.E.R.; Rees, Catherine; Connerton, Ian F.

doi:10.1128/aem.71.8.4885-4887.2005

Cited by 99 publications

(61 citation statements)

References 17 publications

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“…Despite the absence of in vitro susceptibility in isolates of field trial 1, a reduction took place. Other experimental studies in chickens found phages hardly ever lysing Campylobacter isolates of the same source in vitro (16,32,39). Nevertheless, phage findings are associated with reduced numbers of Campylobacter (16,39).…”

Section: Reduction Of Campylobactermentioning

confidence: 94%

“…Their use for reducing Campylobacter in the chicken gut has been investigated in studies with different phages, doses, experimental settings, and application routes. All currently published studies showed promising results with reductions of Campylobacter in the chicken gut of 0.5 to 5.0 log 10 CFU/g (12)(13)(14)(15)(16). Adjusting the dosing methods and timing of previous studies to the conditions in commercial broiler houses plays a major role in further developing bacteriophage-mediated biocontrol of Campylobacter (17).…”

mentioning

confidence: 98%

“…in the avian gut. However, the extent and duration of reduction after dosing were highly variable (12)(13)(14)(15)(16). The field trials were carried out to reassess the results of these studies and to account for the demands of the conditions in commercial broiler production (e.g., no CaCO 3 could be used as buffer in the drinking (Fig.…”

Section: Reduction Of Campylobactermentioning

confidence: 99%

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Effect of Bacteriophage Application on Campylobacter jejuni Loads in Commercial Broiler Flocks

Kittler¹,

Fischer²,

Abdulmawjood

et al. 2013

Appl Environ Microbiol

102

121

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Campylobacteriosis is the most frequent food-borne human enteritis. The major source for infection with Campylobacter spp. is broiler meat. Risk assessments consider the reduction of Campylobacter in primary production to be most beneficial for human health. The aim of this study was to test the efficacy of a bacteriophage application under commercial conditions which had proved to be effective in previous noncommercial studies under controlled experimental conditions. A phage cocktail for Campylobacter reduction was tested on three commercial broiler farms each with a control and an experimental group. Colonization of Campylobacter was confirmed prior to phage application in fecal samples. Subsequently, a phage cocktail was applied via drinking water in the experimental group (log 10 5.8 to 7.5 PFU/bird). One day after phage application, Campylobacter counts of one experimental group were reduced under the detection limit (<50 CFU/g, P ‫؍‬ 0.0140) in fecal samples. At slaughter, a significant reduction of >log 10 3.2 CFU/g cecal content compared to the control was still detected (P ‫؍‬ 0.0011). No significant reduction was observed in the experimental groups of the other trials. However, a significant drop in cecal Campylobacter counts occurred in a phage-contaminated control. These results suggest that maximum reduction of Campylobacter at the slaughterhouse might be achieved by phage application 1 to 4 days prior to slaughter.

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Section: Reduction Of Campylobactermentioning

confidence: 94%

mentioning

confidence: 98%

Section: Reduction Of Campylobactermentioning

confidence: 99%

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Effect of Bacteriophage Application on Campylobacter jejuni Loads in Commercial Broiler Flocks

Kittler¹,

Fischer²,

Abdulmawjood

et al. 2013

Appl Environ Microbiol

102

121

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“…The use of bacteriophages to reduce Campylobacter cecal colonization of chickens has shown encouraging results as far as reducing Campylobacter counts. A number of studies have reported that under experimental conditions, bacteriophage treatment of chickens which have already been colonized with Campylobacter results in reductions of 0.5 to 5.0 log 10 CFU/g Campylobacter in the chicken gut (410)(411)(412)(413)(414). For example, in a field trial by Kittler and colleagues, a phage cocktail at levels of 5.8 to 7.5 log 10 PFU/bird was added to the drinking water of chickens colonized with Campylobacter, and the results showed that, at slaughter, Campylobacter counts in the ceca of treated birds were significantly reduced (Ͼ3.2 log 10 CFU/g cecal content) compared with those of the control group (P ϭ 0.0011) (415).…”

Section: Reducing Campylobacter Transmission In Chickensmentioning

confidence: 99%

Global Epidemiology of Campylobacter Infection

et al. 2015

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SUMMARY Campylobacter jejuni infection is one of the most widespread infectious diseases of the last century. The incidence and prevalence of campylobacteriosis have increased in both developed and developing countries over the last 10 years. The dramatic increase in North America, Europe, and Australia is alarming, and data from parts of Africa, Asia, and the Middle East indicate that campylobacteriosis is endemic in these areas, especially in children. In addition to C. jejuni , there is increasing recognition of the clinical importance of emerging Campylobacter species, including Campylobacter concisus and Campylobacter ureolyticus . Poultry is a major reservoir and source of transmission of campylobacteriosis to humans. Other risk factors include consumption of animal products and water, contact with animals, and international travel. Strategic implementation of multifaceted biocontrol measures to reduce the transmission of this group of pathogens is paramount for public health. Overall, campylobacteriosis is still one of the most important infectious diseases that is likely to challenge global health in the years to come. This review provides a comprehensive overview of the global epidemiology, transmission, and clinical relevance of Campylobacter infection.

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“…Since they are ubiquitous and their prevalence is high in the same environment where their hosts are D DAVID PUBLISHING abundant, bacteriophages can be considered good indicators of the presence of bacteria [23]. For instance it is clearly demonstrated the correlation between coliphages (phages active against Escherichia coli) and bacteria responsible of colibacillosis in animals [24].…”

Section: Introductionmentioning

confidence: 99%

Bacteriophage Morphological Characterization by Using Transmission Electron Microscopy

Aprea¹,

D’Angelo²,

Prencipe³

et al. 2015

JLS

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Bacteriophages or more commonly "phages" are bacterial viruses. They are ubiquitous and good indicators of bacterial contaminations since their prevalence is high in those environments where their hosts are abundant. Phage classification is based on morphology and for this reason, even though it is considered an old technique, TEM (Transmission Electron Microscopy) still plays a key role in their characterization. In the present work, the authors focused on TEM analysis of phage ɸApr-1 isolated against Lactococcus lactis (L. lactis), implicated in industrial fermentations and of phage ɸIZSAM-1, active against Listeria monocytogenes (L. monocytogenes), isolated from the environment. For observation with TEM (EM 900T-Zeiss), phages were harvested in liquid media and were negative stained with fosfotungstic acid 2‰. An accurate viral ultrastructure analysis by using TEM is fundamental not only in the first approach of characterization of newly isolated phages but also for providing useful information to go further to the selection process as potential bio-decontaminants.

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Correlation of Campylobacter Bacteriophage with Reduced Presence of Hosts in Broiler Chicken Ceca

Cited by 99 publications

References 17 publications

Effect of Bacteriophage Application on Campylobacter jejuni Loads in Commercial Broiler Flocks

Effect of Bacteriophage Application on Campylobacter jejuni Loads in Commercial Broiler Flocks

Global Epidemiology of Campylobacter Infection

Bacteriophage Morphological Characterization by Using Transmission Electron Microscopy

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