Correlation of<i>Pseudomonas aeruginosa</i>virulence factors from clinical and environmental isolates with pathogenicity in the neutropenic mouse

Woods, Donald E.; Lam, Joseph S.; Paranchych, W.; Speert, David P.; Campbell, Margaret A.; Godfrey, A J

doi:10.1139/m97-077

Cited by 32 publications

(22 citation statements)

References 42 publications

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“…Observations from clinical experience and a number of infectious models indicate that the virulence of P. aeruginosa varies from strain to strain (32,48,51,60), although the mechanisms accounting for this variation are not completely understood. The genes of most of the characterized P. aeruginosa virulence determinants are located in the core genome and therefore present in all strains (59).…”

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confidence: 99%

Hybrid Pathogenicity Island PAGI-5 Contributes to the Highly Virulent Phenotype of a Pseudomonas aeruginosa Isolate in Mammals

Battle¹,

Meyer

Rello

et al. 2008

J Bacteriol

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Most known virulence determinants of Pseudomonas aeruginosa are remarkably conserved in this bacterium's core genome, yet individual strains differ significantly in virulence. One explanation for this discrepancy is that pathogenicity islands, regions of DNA found in some strains but not in others, contribute to the overall virulence of P. aeruginosa. Here we employed a strategy in which the virulence of a panel of P. aeruginosa isolates was tested in mouse and plant models of disease, and a highly virulent isolate, PSE9, was chosen for comparison by subtractive hybridization to a less virulent strain, PAO1. The resulting subtractive hybridization sequences were used as tags to identify genomic islands found in PSE9 but absent in PAO1. One 99-kb island, designated P. aeruginosa genomic island 5 (PAGI-5), was a hybrid of the known P. aeruginosa island PAPI-1 and novel sequences. Whereas the PAPI-1-like sequences were found in most tested isolates, the novel sequences were found only in the most virulent isolates. Deletional analysis confirmed that some of these novel sequences contributed to the highly virulent phenotype of PSE9. These results indicate that targeting highly virulent strains of P. aeruginosa may be a useful strategy for identifying pathogenicity islands and novel virulence determinants.

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confidence: 99%

Hybrid Pathogenicity Island PAGI-5 Contributes to the Highly Virulent Phenotype of a Pseudomonas aeruginosa Isolate in Mammals

Battle¹,

Meyer

Rello

et al. 2008

J Bacteriol

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“…Possibly, the MAR strain thus was less virulent for the 4 patients, i.e., remained localized in the lower respiratory tract and failed to spread and disseminate [72]. As expected [73], the combined blood/ antibiotic time kill curve assays revealed the combination of amikacin and fosfomycin with added blood to be effective against the two MAR isolates 125 and 127 (table 9).…”

Section: Discussionmentioning

confidence: 80%

Surveillance of <i>Pseudomonas aeruginosa</i> in Intensive Care Units: Clusters of Nosocomial Cross-Infection and Encounter of a Multiple-Antibiotic Resistant Strain

Traub¹,

Scheidhauer²,

Leonhard³

et al. 1998

Chemotherapy

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Serogrouping (determination of O antigens) and bacteriocin typing (based on susceptibility to one or more of 18 bacteriocins) were employed to survey 210 isolates of Pseudomonas aeruginosa from 201 patients in 8 intensive care units (ICU) during an observation period of 18 months. Eighty-eight isolates (41.9%) were nonserogroupable (NT); most common were serogroups O1, O9, O11, and O3. All except 5 isolates (97.6%) were bacteriocin-typable. However, phenotypic variation of bacteriocin susceptibility, in particular the receptor for bacteriocin No. 13, rendered this typing method presumptive as well. Bacteriocin susceptibility profiles were not predictive of serogroup and vice versa. Workup of 19 isolates from 9 patients disclosed phenotypic variation of antibiotic susceptibility in 3 patients, superinfection by a different strain in 4 patients, and persistence (3 months) of the same strain in 2 patients, respectively. Serotyping and bacteriocin susceptibility test data revealed 15 clusters of putative cross-infection of 2 patients each, 8 clusters involving 3 patients each, one outbreak (serogroup NT, bacteriocin profile 777736) involving 4 patients in the pediatric ICU, one outbreak due to a multiple-antibiotic resistant (MAR) strain in the surgical ICU (4 patients, serogroup O12, bacteriocin profile 30400), and two putative outbreaks in the pneumonology ICU involving 6 patients (serogroup NT, bacteriocin profile 777726) and 9 patients (serogroup NT, bacteriocin profile 777736). Pulsed-field gel electrophoresis (PFGE) macrorestriction analysis (SpeI, XbaI) confirmed the pediatric and surgical ICU strains as singular strains. However, the two putative outbreaks in the pneumonology ICU were due to one particular strain which had infected 13 of the 15 patients as determined with the PFGE genotypic method. Isolates comprising the MAR strain of P. aeruginosa were susceptible only to amikacin, fosfomycin, and polymyxin B; the isolates varied in susceptibility to aztreonam and ceftazidime. This MAR strain was susceptible to the bactericidal activity of 65 vol% of fresh defibrinated human blood from donors B, L, and T. Either amikacin (16 µg/ml) or fosfomycin (8 µg/ml) plus blood and amikacin (8 µg/ml) combined with fosfomycin (8 µg/ml) with and without blood consistently killed isolates of the MAR strain, which thus was amenable to antibiotic therapy.

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“…Different in vitro biofilm forming capacities and reduced ability of lung colonization was observed in environmental isolates as compared to clinical isolates by Head and Yu (22). However Woods et al (31) were not able to differentiate both the isolates on the basis of correlation of production of extracellular enzymes and LD50. In this study, we have observed that environmental isolates differ from clinical isolates with respect to their outer membrane protein profiles and their ability to establish in the urinary tract of mouse.…”

Section: Resultsmentioning

confidence: 95%

A Comparative Study of Clinical and Environmental Isolates of Pseudomonas aeruginosa in Terms of Quorum Sensing, Outer Membrane Proteins and Their Ability to Cause Urinary Tract Infection

Kumar¹,

Chhibber²,

Harjai³

2009

Am. J. Biomed. Sci.

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Pseudomonas aeruginosa, one of the most common causes of nosocomial urinary tract infections, is widely distributed in environments. Environmental and clinical isolates have been compared on the basis of production of virulence factors and genomic diversity. However, no studies are available regarding the comparison of their physiological and morphological characteristics. 15 clinical and environmental isolates of Pseudomonas aeruginosa were screened for quorum sensing molecules and all were found to be producers of varied levels of acyl homoserine lactones (AHL's). Both the isolates showed comparable biofilm forming ability but revealed different outer membrane protein profile. Clinical isolate was found to be more virulent than environmental isolate on the basis of urine and renal colonization in experimental urinary tract infection mouse model. Histopathology and other pathology index factors like myeloperoxidase (MPO), malondialdehyde (MDA) and reactive nitrogen intermediate (RNI) level were also found to be more in case of animals infected with clinical isolates. In conclusion, despite similar abilities of biofilm formation and production of quorum sensing signal molecules, clinical isolate was found to have selective advantage in establishing and causing pathology in the urinary tract of mice. Our results indicate that pathogenicity potential of Pseudomonas aeruginosa vary depending on the source of strain as well as its outer membrane protein profile.

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Correlation ofPseudomonas aeruginosavirulence factors from clinical and environmental isolates with pathogenicity in the neutropenic mouse

Cited by 32 publications

References 42 publications

Hybrid Pathogenicity Island PAGI-5 Contributes to the Highly Virulent Phenotype of a Pseudomonas aeruginosa Isolate in Mammals

Hybrid Pathogenicity Island PAGI-5 Contributes to the Highly Virulent Phenotype of a Pseudomonas aeruginosa Isolate in Mammals

Surveillance of <i>Pseudomonas aeruginosa</i> in Intensive Care Units: Clusters of Nosocomial Cross-Infection and Encounter of a Multiple-Antibiotic Resistant Strain

A Comparative Study of Clinical and Environmental Isolates of Pseudomonas aeruginosa in Terms of Quorum Sensing, Outer Membrane Proteins and Their Ability to Cause Urinary Tract Infection

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