INTRODUCTIONIn medical curriculum, methods of assessment have always held most important place in terms of quality of final outcome of medical graduates. For the assessment of cognitive, recall, interpretive and analytical abilities of medical students, the commonest written examination is long and short answer questions.1 However, with advancement of medical technology, these open ended questions have been replaced with structured long or short answer questions bringing more objectivity to the evaluation by the examiners.2,3 Whereas, another most popular form of written assessment method is use of multiple choice questions (MCQs) which are considered to be both reliable and suitable due to its high objectivity and ease of administration. 4,5 Balanced use of multiple tools of assessments are utilized to test various domains of learning as per Bloom's Taxonomy. 6,7 There are ABSTRACT Background: Several studies have indicated gender differences in performance of undergraduate students in medical curriculum mainly in the clinical specialties which requires different set of skills as compared to pre and paraclinical subjects. Therefore, the study aim to investigate gender difference if any, on overall performance as well as on different modalities of assessment like multiple choice questions (MCQ) and structured long & short answer questions (LSQ) of medical students in physiology. Methods: Performance of 238 first year MBBS students in overall theory (MCQ+LSQ) and separately in MCQ and LSQ were compared between male (n=139) & female (n=99). Further, they were sub-grouped on the basis of total marks in theory as low (<50%), medium (50-65%) and high achievers (>65%). Results: Female students scored significantly more compared to male students in total (60.8±7.42% vs 56.81±8.78%) as well as individually in MCQ & LSQ. Also, overall failure rate was more for male (19%) as compared to female (7%). However, comparison of high achiever group revealed no significant difference in performance of both genders in all modalities of assessments. Whereas, female students of both medium and low achiever groups had significantly high scores in LSQ as compared to male, but no such difference was observed for MCQ. Conclusions: Average and below average female students performed better relative to their male counterparts in subjective assessment (LSQ) as compared to assessment demanding more analytical/critical thinking (MCQ). However, performance of high achievers did not show any such gender difference. Therefore, different types of assessments bring out different abilities of students across genders depending on their grade of performance in a medical curriculum.