Correlation of NO Metabolites and 8-Iso-Prostaglandin F _2a With Periventricular Hyperintensity Severity

Abstract

Objective-Oxidative stress and NO are thought to play important roles in arteriosclerosis pathogenesis, a major cause of white matter lesions in the brain. Therefore, we examined whether NO metabolites (NOx) and 8-iso-prostaglandin F 2␣ (IsoP) levels in vivo correlated with the severity of periventricular hyperintensity (PVH) to evaluate potential roles of oxidative stress and NO in white matter lesions. Methods and Results-Participants (687 males and 528 females) of a health-screening examination were recruited into the study. The plasma NOx and urinary IsoP levels were measured using the Griess method and ELISA, respectively. PVH was diagnosed on the basis of MRIs. In nonparametric univariate trend analyses, plasma NOx as well as aging, presence of hypertension and of lacunes, mean blood pressure, and high-density lipoprotein cholesterol showed highly significant monotone correlation with PVH severity (PՅ0.01). By the multivariate ordinal regression analysis, the plasma NOx (Pϭ0.002) and urinary IsoP (Pϭ0.01) levels were found to be independent factors influencing the severity of PVH together with aging (PϽ0.001), presence of hypertension (PϽ0.001) and lacunes (PϽ0.001), and mean blood pressure (Pϭ0.001). Conclusions-Oxidative stress and NO have a close correlation with PVH severity. (Arterioscler Thromb Vasc Biol.

The Pathobiology of Vascular Dementia

“…6). Markers of endothelial activation, hemostasis, inflammation and oxidative stress are also upregulated in blood, consistent with more widespread effects in the systemic circulation (Gallacher et al, 2010; Knottnerus et al, 2010; Markus et al, 2005; Rouhl et al, 2012a; Shibata et al, 2004; Xu et al, 2010) (fig. 6).…”

Cardiovascular risk factors and small vessel disease of the brain: Blood pressure, white matter lesions, and functional decline in older persons

“…Other inflammatory and prothrombotic markers that have been correlated with WMH lesions in smaller studies include plasma nitric oxide metabolites and urinary 8-iso-prostaglandin F2α and prothrombin factors, von Willebrand Factor, and plasminogen activator inhibitor-1 (PAI-1). 45,46 Lipoproteins. Similar to the glycemic data noted above, studies have shown heterogeneous findings relating lipid abnormalities to small vessel disease of the brain.…”

Effects of Six Functional SNPs on the Urinary 8-Isoprostane Level in a General Japanese Population; Shimane COHRE Study

“…The urinary IsoP level is thought to be a good marker for the oxidative stress in population studies because IsoP is a stable substance in the urine even under the room temperature [11]. Further, several reports indicated that the urinary IsoP level was a risk factor for cardiovascular diseases, such as cerebrovascular disease and myocardial infarction [13,14].…”

“…IsoP is an isomer of prostaglandin F2α, which is generated through non-enzymatic peroxidation of arachidonic acid by the oxidative stress [11]. This substance is stable in the urine and is a good marker for the oxidative stress under the epidemiological study design [12][13][14][15].…”