Correlation of Pretreatment Polarographically Measured Oxygen Pressures with Quantified Contrast-Enhanced Power Doppler Ultrasonography in Spontaneous Canine Tumors and their Impact on Outcome After Radiation Therapy

Bley, Carla Rohrer; Laluhová, Dagmar; Roos, Małgorzata; Kaser‐Hotz, Barbara; Ohlerth, Stefanie

doi:10.1007/s00066-009-1988-6

Cited by 7 publications

(6 citation statements)

References 40 publications

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“…In a study of the efficacy of sorafenib treatment of metastatic renal cancer in humans, the correlation between treatment outcome and the percentage of perfused tissue decreased 3 weeks after treatment (15). In contrast, in studies evaluating DCE-US after radiotherapy for spontaneous tumors in dogs (18,81), DCE-US results were not predictive of disease outcome. This difference can be explained by an important difference in the mechanism of action of radiotherapy compared to ECT combined with GET pIL-12.…”

Section: Discussionmentioning

confidence: 94%

“…Dynamic contrast-enhanced ultrasound (DCE-US) is a simple, readily available, non-invasive, safe, and inexpensive method for assessing tissue perfusion at the capillary level and correlates with histological results of vessel density in preclinical (1)(2)(3) and clinical studies (4)(5)(6)(7). Moreover, DCE-US has been used to predict the efficacy of antiangiogenic treatments of various tumors in preclinical (3) and human clinical trials (8)(9)(10)(11)(12)(13)(14)(15)(16)(17), whereas DCE-US had no predictive value in canine tumors treated with radiotherapy (18). Furthermore, DCE-US results correlated with the results of advanced diagnostic imaging (19).…”

Section: Introductionmentioning

confidence: 99%

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Results of Dynamic Contrast-Enhanced Ultrasound Correlate With Treatment Outcome in Canine Neoplasia Treated With Electrochemotherapy and Interleukin-12 Plasmid Electrotransfer

et al. 2021

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Electrochemotherapy (ECT) and/or gene electrotransfer of plasmid DNA encoding interleukin-12 (GET pIL-12) are effective treatments for canine cutaneous, subcutaneous, and maxillofacial tumors. Despite the clinical efficacy of the combined treatments of ECT and GET, data on parameters that might predict the outcome of the treatments are still lacking. This study aimed to investigate whether dynamic contrast-enhanced ultrasound (DCE-US) results of subcutaneous tumors differ between tumors with complete response (CR) and tumors without complete response (non-CR) in dogs treated with ECT and GET pIL-12. Eight dogs with a total of 12 tumor nodules treated with ECT and GET pIL-12 were included. DCE-US examinations were performed in all animals before and immediately after therapy as well as 8 h and 1, 3, and 7 days later. Clinical follow-up examinations were performed 7 and 14 days, 1 and 6 months, and 1 year after treatment. Numerous significant differences in DCE-US parameters were noted between tumors with CR and non-CR tumors; perfusion and perfusion heterogeneity were lower in CR tumors than in non-CR tumors. Therefore, studies with larger numbers of patients are needed to investigate whether DCE-US results can be used to predict treatment outcomes and to make effective decisions about the need for repeated therapy or different treatment combinations in individual patients.

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Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Results of Dynamic Contrast-Enhanced Ultrasound Correlate With Treatment Outcome in Canine Neoplasia Treated With Electrochemotherapy and Interleukin-12 Plasmid Electrotransfer

et al. 2021

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“…For a subset of the tumors from client-owned dogs, tissue hypoxia levels measured with the Eppendorf polarographic oxygen technique were available from a previous trial [ 7 ] along with tumor biopsies taken at the same time point and in the same tumor region. Formalin-fixed tissue blocks were available from the latter to evaluate the immunohistochemical markers.…”

Section: Methodsmentioning

confidence: 99%

“…Perfusion data was available for a subset of tumor samples as part of a previous trial [ 7 ] in client-owned dogs mentioned above. A 5- to 12-MHz linear transducer (ATL 5000, Philips AG, Zurich, Switzerland) was used to perform imaging.…”

Section: Methodsmentioning

confidence: 99%

“…For this study, dogs with malignant oral tumors were chosen as a large animal model for the investigation of microenvironmental parameters as they are uniquely suited for repetitive minimally invasive and non-invasive observation during radiotherapy [ 6 , 7 ]. In contrast to xenograft studies, canine tumors develop naturally and grow over long periods of time in the presence of an intact immune system, sharing similarities with human neoplasms such as inter-patient tumoral heterogeneity [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Hypoxia-Related Marker GLUT-1, CAIX, Proliferative Index and Microvessel Density in Canine Oral Malignant Neoplasia

et al. 2016

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For various types of tumor therapy, it is suggested that co-targeting of tumor microenvironment, mainly tumor vasculature, mediates tumor response mechanisms. Immunohistochemistry for glucose transporter-1 (GLUT-1), carbonic anhydrase-IX (CAIX), Ki-67, and von Willebrand factor VIII for microvessel density (MVD) were performed on formalin-fixed paraffin-embedded samples of canine oral malignant neoplasms. Polarographic oxygen measurements (median pO2) and perfusion data via contrast-enhanced power Doppler ultrasound (median vascularity, median blood volume) provided additional information. Ninety-two samples were analyzed: sarcomas (n = 32), carcinomas (n = 30), and malignant melanomas (n = 30). Polarographic oxygen and perfusion data was available in 22.8% (sarcomas n = 9, carcinomas n = 7, melanomas n = 5), and 27.1% (sarcomas n = 10, carcinomas n = 8, melanomas n = 7) of cases, respectively. GLUT-1 expression was detected in 46.7% of all samples, and was generally weak. CAIX expression was found in 34.8% of all samples. Median Ki-67 score and MVD count was 19% and 17, respectively. The evaluation of the GLUT-1 score and continuous data showed significantly lower GLUT-1 levels in sarcomas (mean 5.1%, SD 6.2) versus carcinomas and melanomas (mean 16.5%/ 19.0%, SD 17.3/ 20.9, p = 0.001). The expression of CAIX correlated mildly positively with GLUT-1 (p = 0.018, rho = 0.250) as well as with Ki-67 (p = 0.014, rho = 0.295). MVD showed a significantly lower level in melanomas (mean 12.6, SD 7.7) versus sarcomas and carcinomas (mean 21.8/ 26.9, SD 13.0/20.4, p = 0.001). Median vascularity and blood volume were significantly lower in sarcomas (mean 10.4%, SD 11.0, and mean 6.3%, SD 6.5, respectively) versus carcinomas (mean 39.2%, SD 16.4 and mean 33.0%, SD 25.6, respectively) and melanomas (mean 36.0%, SD 18.3, and 31.5%, SD 24.5). Between the 3 histological groups, there was neither a significant difference in the GLUT-1 and CAIX score and continuous data, nor the Ki67 score, or polarographic oxygen measurements. GLUT-1 continuous data and Ki-67 (p<0.001, rho = 0.403), as well as Ki-67 and MVD (p = 0.029, rho = 0.228) correlated positively and a mild correlation was found between vascularity and GLUT-1 (p = 0.043, rho = 0.408). GLUT-1, CAIX, proliferative index and MVD levels were established as microenvironmental descriptors with the purpose of creating a baseline in order to follow changes seen in the tumor microenvironment after hypofractionated radiation with high doses.

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Quantitative assessment of hypoxia subtypes in microcirculatory supply units of malignant tumors Using (immuno-)fluorescence techniques

et al. 2011

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Correlation of Pretreatment Polarographically Measured Oxygen Pressures with Quantified Contrast-Enhanced Power Doppler Ultrasonography in Spontaneous Canine Tumors and their Impact on Outcome After Radiation Therapy

Abstract: These analyses show that quantified contrast-enhanced power Doppler ultrasonography does not represent a noninvasive indirect method to assess tumor hypoxia measured by invasive pO(2) histography. Both technologies were nonprognostic indicators in spontaneous malignant canine oral tumors.

Cited by 7 publications

References 40 publications

Results of Dynamic Contrast-Enhanced Ultrasound Correlate With Treatment Outcome in Canine Neoplasia Treated With Electrochemotherapy and Interleukin-12 Plasmid Electrotransfer

Results of Dynamic Contrast-Enhanced Ultrasound Correlate With Treatment Outcome in Canine Neoplasia Treated With Electrochemotherapy and Interleukin-12 Plasmid Electrotransfer

Hypoxia-Related Marker GLUT-1, CAIX, Proliferative Index and Microvessel Density in Canine Oral Malignant Neoplasia

Quantitative assessment of hypoxia subtypes in microcirculatory supply units of malignant tumors Using (immuno-)fluorescence techniques

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