Correlation of proline content and gene expression involved in the metabolism of this amino acid under abiotic stress

Abstract

This study evaluated the metabolism of proline in rice based on the expression pattern of genes involved in biosynthesis and catabolism of amino acid and determined the correlation with the proline content produced under stress. In stage V4, genotypes with a differentiated response to salinity and low-temperature stresses were exposed separately to two test conditions: 150 mM NaCl and 13°C. Proline increased in both genotypes and stresses. The expression of P5CS1 was increased in both genotypes and conditions, while P5CS2 was responsive in the sensitive genotype under salinity. High correlations between proline content/P5CS1, proline content/P5CS2, P5CS1/P5CR, and P5CS2/P5CR were observed for the tolerant genotype under salt stress. The correlation between proline content/PDH was 0.42 and-0.13 (tolerant genotype) and-0.54 and 0.15 (sensitive genotype) under salinity and cold, respectively. P5CDH was positively correlated with proline content under salinity for both genotypes. Under salt stress, there was a positive correlation between proline content/OAT in the tolerant genotype. It was concluded that the increase in proline content is greater for the tolerant genotype. Under salt stress, the levels of transcript genes are more correlated with the proline content in the tolerant genotype, while at 13°C, the correlation of the sensitive genotype was higher.

Transcriptome analysis and molecular mechanism of linseed (Linum usitatissimum L.) drought tolerance under repeated drought using single-molecule long-read sequencing

“…(Figure 9a). P5CR gene family members encode the last enzyme in the plant proline biosynthesis pathway, overexpression P5CR gene will signi cantly improve the photosynthetic response of Arabidopsis under drought and hightemperature stress [27,28]. In this study, we found that the expression level of P5CR gene, such as Lus10034453, in Z141 was signi cantly higher than that in NY-17 under drought stress, and its expression pattern also matched our drought treatment model (Figure 9b).…”

Evaluation of Mitigation Effects of Glomus Mosseae on Triticum Aestivum L., Cv. Chamran Under Drought Stress

“…Chamran. In accordance to our results, Porcel and Ruiz-Lozano (2004) recorded a decrease in proline concentration in AM-inoculated Glycine max L. Additionally, similar results have been reported by Asrar et al (2012) in Antirrhinum majus L. and Amiri et al (2017) in Pelargonium graveolens L. There is some ambiguity in the actual association of proline with tolerance to stresses (Ashraf and Foolad 2007;Benitez et al, 2016), while some scholars postulated that increase in proline level is indeed related to a specific level of tolerance, while others have found the proline aggregation as a signal of injury (Hien et al, 2003;Silvente et al, 2012). Carbohydrates are of the critical primary compounds to promote various physiological tolerant processes under abiotic stresses (Amiri et al 2017).…”

Transcriptome analysis and molecular mechanism of linseed (Linum usitatissimum L.) drought tolerance under repeated drought using single-molecule long-read sequencing

“…9a). P5CR gene family members encode the last enzyme in the plant proline biosynthesis pathway, overexpression P5CR gene will significantly improve the photosynthetic response of Arabidopsis under drought and hightemperature stress [30,31]. In this study, we found that the expression level of P5CR gene, such as Lus10034453, in Z141 was significantly higher than that in NY-17 under drought stress, and its expression pattern also matched our drought treatment model (Fig.…”