Correlation of renal solute-free water reabsorption and energy of interaction of vasotocin analogs with V2 receptor

Kutina, A. V.; Karavashkina, T. A.; Ei, Shakhmatova; Gao, Jian; Mordvintsev, D. Yu.; Kuzmin, Dmitry; Tsetlin, Victor I.; IuV, Natochin

doi:10.1134/s1062359011060057

Cited by 2 publications

(1 citation statement)

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“…Peptides were dissolved in 0.9% NaCl and tested in vivo at two different doses ( n = 10 rats in each group). The experimental design was based on our previous data . The effect of peptides on urinary sodium and potassium excretion was examined after the injection of the analogues at a dose 0.05 nmol per 100 g body weight (bw).…”

Section: Methodsmentioning

confidence: 99%

Synthesis of new vasotocin analogues: effects on renal water and ion excretion in rats

Kutina

Marina

Eliseev

et al. 2013

Journal of Peptide Science

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Vasopressin and nonmammalian hormone vasotocin are known to increase the water permeability of mammalian collecting ducts, frog skin and the urinary bladder. Neurohypophysial nonapeptides have also been shown to interfere with the regulation of renal ion transport. The subject of this study was a search for vasopressin and vasotocin analogues with selective effects on renal water, sodium and potassium excretion. During this study, we synthesised the following peptides: 13 vasotocin analogues modified at positions 4 (Thr or Arg), 7 (Gly or Leu) and 8 (D-Arg, Lys or Glu); 4 vasopressin analogues modified at positions 4 and 8; and 9 peptides shortened or extended at the C-terminal or with substitutions for Gly-NH2. Most of these peptides had mercaptopropionic acid (Mpa) instead of Cys in position 1. The effects of these nonapeptides on renal water, sodium and potassium transport were evaluated in in vivo experiments using Wistar rats. Some nonapeptides possessed antidiuretic, natriuretic and kaliuretic activities ([Mpa(1)]-arginine vasotocin, [Mpa(1), homoArg(8)]-vasotocin, [Mpa(1), Thr(4)]-arginine vasotocin and [Mpa(1), Arg(4)]-arginine vasopressin). Substitutions at positions 4 and 8 increased the selectivity of peptide actions. The antidiuretic [D-Arg(8)]-vasotocin analogues had no effects on sodium excretion. [Mpa(1), Arg(4)]-arginine vasotocin was antidiuretic and kaliuretic but not natriuretic. [Mpa(1), Glu(8)]-oxytocin had weak natriuretic activity without any effects on water and potassium transport. In accordance with the data obtained, synthesised vasotocin analogues could be good candidates for pharmaceuticals selectively regulating renal sodium and potassium transport, which is of clinical importance.

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Section: Methodsmentioning

confidence: 99%