Correlation of serology with morphological changes in gastric biopsy in Helicobacter pylori infection and evaluation of immunohistochemistry for H. pylori identification

Shukla, Shailaja; Agarwal, Aditya; Pujani, Mukta; Rohtagi, Anurag

doi:10.4103/1319-3767.103428

Cited by 23 publications

(5 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…this outcome was similar to [11] who identified 66.13% of serum Anti-Helicobacter pylori IgG and 12% of IgM by ELISA and also comparable to [12] who detected the highest seroreactive level of IgG and IgA in patients who suffered from antral gastritis with the recording of significant association between antral gastritis parameter and ELISA IgG. The current study result was similar to [13] who demonstrated the high titer of IgG 72% and low titer of IgM 36% by ELISA and obtained notable correlation between anti-H.pylori IgG and antral gastritis. Many studies' findings disagreed with the result of the present study as in [14] who found the highest level of IgM 63.33%, and IgG 13.33% by ELISA technique.…”

Section: Resultssupporting

confidence: 90%

Gastroduodenal endoscopic findings and Seroprevalence of Helicobacter pylori among suspected individuals

Al-barwary¹,

Saadi²,

Salih³

et al. 2022

KUJSS

View full text Add to dashboard Cite

The peptic ulcer was developed and appeared recently as a contagious disease, distributed all over the world and the most common cause of this disease is H.pylori. Laboratory diagnosis was used to detect this bacterium by several methods which include invasive and non-invasive methods. ELISA technique was used to identify IgG, IgM, and IgA antibodies against H.pylori. All participants underwent endoscopic procedures at the Duhok City/Azadi Hospital/Endoscopy Center, and a highly skilled endoscopist carried out the procedures, As well questionnaire form has been filled out through the interview with the participants to enroll the socio-demographic factors which include occupation, history of treatment, and endoscopic findings. Among the 90 participants, the serological prevalence is 72%, 7% and 30% of positive antibodies IgG, IgM, and IgA respectively. The housewife group recorded a high percentage of positive IgG whereas low percentages were recorded for the military group. A significant immune reaction of IgG in the student group has been noted. In addition to the housewife group to the positive IgM. The effect of those who had treatment previously has been revealed by positive IgG. Moreover, antral gastritis had a significant relationship with positive IgG by using ELISA. in the conclusion, the Housewives group was more infected with this bacterium, and a significant association was found between antral gastritis and seropositive IgG.

show abstract

Section: Resultssupporting

confidence: 90%

Gastroduodenal endoscopic findings and Seroprevalence of Helicobacter pylori among suspected individuals

Al-barwary¹,

Saadi²,

Salih³

et al. 2022

KUJSS

View full text Add to dashboard Cite

show abstract

“…The reported sensitivities of histochemical methods range between 60% and 90%, which is similar to our determined sensitivity of 64.5%. 10,[24][25][26] Our findings show that CV staining is a poor ancillary diagnostic test for detecting H. pylori, particularly when there is a low bacterial load present. IHC staining showed a sensitivity almost double that of CV staining (67.0% vs. 33.7%) at a low organism load.…”

Section: Discussionmentioning

confidence: 99%

Role of Helicobacter pylori Immunohistochemistry in the Histopathological Assessment of Inflamed Endoscopic Gastric Biopsies

Hall,

Bruce-Brand,

Mudini

et al. 2024

Korean J Helicobacter Up Gastrointest Res

View full text Add to dashboard Cite

Objectives: The identification of Helicobacter pylori is one of the main tasks of diagnostic histopathologists when evaluating endoscopic gastric biopsies. The sensitivity and specificity of different stains that facilitate this identification vary. Despite the existing guidelines, many histopathology laboratories perform routine histochemical staining of all gastric biopsies to improve turnaround times. This study assessed the utility of an H. pylori immunohistochemical (IHC) stain compared with a routinely used histochemical stain, cresyl violet (CV), in the South African setting.Methods: Cases were identified retrospectively, and original histopathology reports were used to establish the “ground truth” diagnoses. Three pathologists independently evaluated the CV and IHC stains; each pathologist was timed in a standardized manner. The sensitivity, specificity, interobserver variability, and time taken to identify H. pylori with each stain were compared.Results: The overall sensitivity and specificity for IHC staining (85.2% and 97.7%, respectively) were higher than those for CV staining (64.5% and 90.6%, respectively). Detection of H. pylori took an average of 16 and 49 seconds using the IHC and CV stains, respectively. The prevalence of H. pylori in our laboratory was 23.7%, which is lower than the reported national prevalence in South Africa.Conclusions: IHC stain-based detection of H. pylori in inflamed gastric biopsies demonstrated superior sensitivity and specificity than CV staining. This was particularly true for cases involving patients with low bacterial loads. The interpretation of H. pylori IHC staining is much faster than that associated with CV staining, which is important in centers with high caseloads and shortages of pathologists.

show abstract

“…Gastric fragments were obtained during endoscopy from five different sites as recommended by the Updated Sydney System for classification of gastritis. [ 9 ] A number of clinicopathological variables such as gender, age, tumor location, histological type, tumor-node-metastasis (TNM) stage, depth of tumor invasion, lymph node metastasis, distant metastasis, and vascular invasion were obtained from the histopathological records and included for survival analysis. The tissue sections were also assessed for the presence of H. pylori infection by immunohistochemical staining using polyclonal anti- H. pylori antibody.…”

Section: Methodsmentioning

confidence: 99%

MicroRNA-155-enhanced autophagy in human gastric epithelial cell in response to Helicobacter pylori

Zhu

Yao

et al. 2016

Saudi J Gastroenterol

View full text Add to dashboard Cite

Background/Aim:MicroRNAs (miRNAs) are a class of small noncoding RNAs acting as posttranscriptional gene expression regulators in many physiological and pathological conditions. MiR-155 is one kind of miRNAs that plays an important role in causing various diseases. However, the precise molecular mechanism of the ectopic expression of miR-155 in Helicobacter pylori infection remains poorly understood. Autophagy has recently been identified as an effective way to control the intracellular bacterium survival. In the present study, we demonstrate a novel role of miR-155 in regulating the autophagy-mediated anti-H. pylori response.Patients and Methods:Totally 86 H. pylori-positive patients together with 10 H. pylori-negative, healthy control subjects were included in the study. Correlation between immunohistochemical grades and miR-155 expression were determined. Molecular mechanism of miR-155 on regulation of autophagy and elimination of intracellular H. pylori were determined using the GES-1 cell model.Results:We found that overexpression of miR-155 by transfecting miR-155 mimics could significantly decrease the survival of intracellular H. pylori, and this process was through induction of autophagy. Furthermore, there was a significant correlation between miR-155 and immunohistochemical grades in H. pylori-positive patients, and miR-155 expression were decreased in the intestinal metaplasia group.Conclusions:The results have indicated that the miR-155 expression level plays a key role in immunity response against H. pylori and this might provide potential targets for the future treatment of H. pylori-related diseases.

show abstract

Correlation of serology with morphological changes in gastric biopsy in Helicobacter pylori infection and evaluation of immunohistochemistry for H. pylori identification

Cited by 23 publications

References 31 publications

Gastroduodenal endoscopic findings and Seroprevalence of Helicobacter pylori among suspected individuals

Gastroduodenal endoscopic findings and Seroprevalence of Helicobacter pylori among suspected individuals

Role of Helicobacter pylori Immunohistochemistry in the Histopathological Assessment of Inflamed Endoscopic Gastric Biopsies

MicroRNA-155-enhanced autophagy in human gastric epithelial cell in response to Helicobacter pylori

Contact Info

Product

Resources

About