Correlation of serum alkaline phosphatase activity with the healing process of long bone fractures in dogs

Komnenou, Anastasia; Karayannopoulou, Maria; Polizopoulou, Z. S.; Constantinidis, Theodore C.; Dessiris, A.

doi:10.1111/j.1939-165x.2005.tb00006.x

Cited by 62 publications

(48 citation statements)

References 14 publications

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“…In a study performed by Komnenou et al (2005) on dogs, and in one performed by Singh Ajai et al (2013) on human patients, the serum alkaline phosphatase (ALP) activity was determined throughout the healing process of long bone diaphyseal fractures. In both studies it was observed that serum ALP activity remained within the reference limits during the entire postoperative period in patients that had developed a non-union process, probably indicating a suppression of osteoblastic activity.…”

Section: Discussionmentioning

confidence: 99%

Bone turnover markers for early detection of fracture healing disturbances: A review of the scientific literature

Sousa

Dias

López-Peña

et al. 2015

An. Acad. Bras. Ciênc.

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Imaging techniques are the standard method for assessment of fracture healing processes. However, these methods are perhaps not entirely reliable for early detection of complications, the most frequent of these being delayed union and non-union. A prompt diagnosis of such disorders could prevent prolonged patient distress and disability. Efforts should be directed towards the development of new technologies for improving accuracy in diagnosing complications following bone fractures. The variation in the levels of bone turnover markers (BTMs) have been assessed with regard to there ability to predict impaired fracture healing at an early stage, nevertheless the conclusions of some studies are not consensual. In this article the authors have revised the potential of BTMs as early predictors of prognosis in adult patients presenting traumatic bone fractures but who did not suffer from osteopenia or postmenopausal osteoporosis. The available information from the different studies performed in this field was systematized in order to highlight the most promising BTMs for the assessment of fracture healing outcome.

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Section: Discussionmentioning

confidence: 99%

Bone turnover markers for early detection of fracture healing disturbances: A review of the scientific literature

Sousa

Dias

López-Peña

et al. 2015

An. Acad. Bras. Ciênc.

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“…These markers are also used in several animal species in clinical and orthopaedic research, particularly in sheep (Newman et al 1995, Chavassieux et al 1997, Turner 2002, Taniguchi et al 2003, Klein et al 2004, Komnenou et al 2005, Seebeck et al 2005.…”

mentioning

confidence: 99%

“…Sheep have also been used as an experimental model in orthopaedic studies, with assessment of bone healing usually acquired by means of imaging, histological and histomorphometric techniques and mechanical tests . Nevertheless, measurements of serum markers of bone turnover could possibly also aid in the evaluation of cellular function in fracture or bone defect healing research, supplying information about the organic response to the lesion and to the selected treatment (Taniguchi et al 2003, Klein et al 2004, Komnenou et al 2005, Seebeck et al 2005.…”

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confidence: 99%

Assessment of markers of bone formation under controlled environmental factors and their correlation with serum minerals in adult sheep as a model for orthopaedic research

et al. 2008

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SummaryEighteen healthy skeletally mature (3 years old) ewes, with an average weight of 45 kg, of the Portuguese Churra da Terra Quente breed were used to evaluate the normal values of total and bone-specific isoform of alkaline phosphatase serum activities (ALP and BALP, respectively) and serum osteocalcin (OC) and their correlation with the serum mineralscalcium (Ca), phosphorus (P), magnesium (Mg) and ionized calcium (Ca 2þ ). The sheep were maintained under controlled environmental conditions (constant diurnal photoperiod cycle and identical husbandry and feeding) for six weeks before the collection of the blood samples. The measurement of the total ALP and serum minerals was performed with automated biochemistry analysers using the BioMé rieux w kits, the serum electrolyte Ca 2þ Diametrics Medical, Inc w specific cassettes and the BALP and OC METRATM kits from QUIDEL Keywords Total and bone-specific alkaline phosphatase; osteocalcin; minerals; serum; sheepThe biochemical markers of bone metabolism reflect the bone turnover process and are usually divided into formation and resorption markers. Formation markers are proteins or enzymes secreted by osteoblasts during the bone formation process, such as serum total and bone-specific isoform of alkaline phosphatase (BALP), serum osteocalcin (OC) and serum procollagen type I C-and N-terminal propeptides. The latter two molecules are released during the type I collagen molecule formation. Bone resorption markers are those resulting from the breakdown of type I collagen

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“…Sendo a fosfatase alcalina um indicador de atividade osteoblástica durante a reparação de fratura, o aumento dos níveis séricos concomitante á formação do calo ósseo sugerem deposição de matriz óssea (KOMNENOU et al, 2005), porém a grande variação dos valores entre os indivíduos remete a graus variados de atividade e pode diminuir a importância de tais resultados. Conforme Carano e Filvaroroff (2003) e Doblaré et al (2004), o calo ósseo encontra-se formado ao fi nal da segunda ou terceira semana e a partir deste período ocorre diminuição da atividade osteoblástica.…”

Section: Resultsunclassified

<b>Perfil sérico da fosfatase alcalina após o reparo de defeitos osteocondrais com células mononucleares da medula óssea e proteína morfogenética óssea em coelhos</b><br>DOI:10.5007/2175-7925.2011v24n4p103

et al. 2011

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ResumoVerifi cou-se a atividade da fosfatase alcalina sérica em resposta ao reparo de defeitos osteocondrais em vinte e quatro coelhos da raça Nova Zelândia. Distribuídos em três grupos, o controle (GC), o tratado com células mononucleares da medula óssea (GCM) e o que recebeu células mononucleares autólogas com proteína morfogenética óssea (GCM+BMP). Após exposição do sulco troclear da articulação fêmoro-tíbio-patelar esquerda removeu-se um segmento em forma de cunha. O local foi preenchido com enxerto osteocondral alógeno conservado em glicerina a 98%. No GC foi realizada a enxertia óssea, no GCM além do enxerto foi inoculado 2x10 6 células mononucleares e no GCM+BMP foi administrada intra-articularmente o mesmo número de células associadas à 1μg de proteína morfogenética óssea. A resposta osteoblástica foi mensurada por meio da análise sérica de fosfatase alcalina no dia 0 (pré-operatório) 3, 15, 30, 45 após cirurgia, além do acompanhamento por exames radiográfi cos. Procedeu-se a análise de variância em blocos ao acaso, esquema fatorial e teste de Tukey (p=0,05). A média geral do GCM foi superior quando comparada aos outros grupos e as maiores taxas estiveram entre o 15º e 45º dias de pós-operatório. A discrepância dos valores entre os indivíduos do mesmo grupo proporciona dúvidas na veracidade do teste.Palavras-chave: Células-tronco, Consolidação de fraturas, Marcador ósseo Biotemas, 24 (4): 103-109, dezembro de 2011

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Correlation of serum alkaline phosphatase activity with the healing process of long bone fractures in dogs

Abstract: Serial determination of serum ALP activity during fracture healing could be an additional tool in predicting fractures at risk of developing a nonunion, helping the clinician to choose the appropriate intervention.

Cited by 62 publications

References 14 publications

Bone turnover markers for early detection of fracture healing disturbances: A review of the scientific literature

Bone turnover markers for early detection of fracture healing disturbances: A review of the scientific literature

Assessment of markers of bone formation under controlled environmental factors and their correlation with serum minerals in adult sheep as a model for orthopaedic research

<b>Perfil sérico da fosfatase alcalina após o reparo de defeitos osteocondrais com células mononucleares da medula óssea e proteína morfogenética óssea em coelhos</b><br>DOI:10.5007/2175-7925.2011v24n4p103

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