Correlation of two anthocyanin quantification methods: HPLC and spectrophotometric methods

Lee, Jungmin; Rennaker, Christopher; Wrolstad, Ronald E.

doi:10.1016/j.foodchem.2008.03.010

Cited by 257 publications

(180 citation statements)

References 13 publications

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“…Os valores de pH dos extratos diluídos foram de 1,0 (tampão cloreto de potássio 0,025 Mol L -1 ) e de 4,5 (tampão acetato de sódio 0,4 Mol L -1 ). Os valores de absorbância (A) das amostras diluídas foram calculados, como segue: O conteúdo de antocianinas foi calculado como mg 100 g -1 de malvidina-3-glicosídeo, usando-se o coeficiente de absortividade molar (ε) de 28000 e o peso molecular (PM) de 493,5 (Lee et al, 2008), conforme a seguinte fór-mula:…”

Section: Methodsunclassified

Teores de antocianinas, fenóis totais, taninos e ácido ascórbico em uva 'bordô' sobre diferentes porta-enxertos

2012

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Teores de antocianinas, fenóis totais, taninos e ácido ascórbico em uva 'bordô' sobre...Rev. Ceres, Viçosa, v. 59, n.5, p. 701-708, set/out, 2012 RESUMO ABSTRACT Anthocyanins, total phenols, tannins and ascorbic acid in cv. Bordô grape on different rootstocksThis study aimed to evaluate the content of anthocyanins, total phenols, tannins and ascorbic acid in grape grown onto '420A', 'IAC-766' and 'Ripária-of-Traviú' rootstocks in three different harvest periods. The experiment was conducted in 'Bordo' grapes vineyard in Missal city, Paraná West, in the 2007/2008 harvest. The rootstock effect was evaluated in three periods after flowering: 90 days, with ripening berries , tending to a definite purple; 100 days, with berries completely purple , and 110 days, with berries overripening. The experiment was arranged in a split plot design in randomized blocks. The variables soluble solids, titratable acidity, total phenols index, anthocyanins, tannins and ascorbic acid were analyzed. The results showed that in the rootstocks '420A' and 'IAC-766', the 'Bordô' grape showed similar responses for anthocyanins, tannins, acidity and soluble solids accumulation, but the contents of phenolic compounds in the pulp and epidermis of berries were higher in '420A'. On the rootstock 'Ripária-do-Traviú', the 'Bordô' grape showed lower accumulation of anthocyanins, total phenols and tannins in the epidermis. Higher accumulations of ascorbic acid were found in 'Bordô' grapes on the rootstock 'IAC-766'. There is evidence suggesting that the 'Ripária-do-Traviú' rootstock as inferior to others in nutritional and enological aspects. Este trabalho teve como objetivo avaliar o conteúdo de antocianinas, fenóis totais, taninos e ácido ascórbico, em variedade de uva 'Bordô', cultivada sobre os porta-enxertos '420A', 'IAC-766' e 'Ripária-do-Traviú', em três períodos de colheita distintos. O experimento foi realizado em vinhedo de uva 'Bordô', no município de Missal, no Oeste do Paraná, na safra 2007/2008. O efeito dos porta-enxertos foi avaliado em três períodos de colheita, a partir da época de floração: 90 dias, com bagas em amadurecimento, tendendo ao roxo definitivo; 100 dias, com bagas totalmente roxas; e 110 dias, com bagas em sobrematuração. Foi utilizado o delineamento em blocos casualizados, em parcelas subdivididas no tempo. As variáveis analisadas foram sólidos solúveis, acidez titulável, índice de fenóis totais, antocianinas, taninos e teor de ácido ascórbico. Os resultados mostraram que, nos porta-enxertos '420A' e 'IAC-766', a uva 'Bordô' apresentou respostas similares em acúmulos de antocianinas, taninos, acidez titulável e sólidos solúveis, mas os acúmulos de fenóis totais, tanto na polpa, quanto na epiderme das bagas, foram maiores no '420A'. Ocorreram menores acúmulos de antocianinas, fenóis totais e taninos da epiderme da uva 'Bordô', quando enxertada em 'Ripária-doTraviú'. Acúmulos superiores de ácido ascórbico foram verificados nas uvas enxertadas em 'IAC-766'. Há evidências que apontam o porta-enxerto 'Ripária-do-Traviú' c...

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Section: Methodsunclassified

Teores de antocianinas, fenóis totais, taninos e ácido ascórbico em uva 'bordô' sobre diferentes porta-enxertos

2012

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“…A contributing factor is the difference in calculating total anthocyanidins by summation of individual anthocyanidin amounts compared to using the pH differential method which calculates total anthocyanidins as one group of compounds based on UV spectra [59,60]. In general, blueberry results reported from a spectrophotometric method are higher in the same sample than results from methods Figure 6.…”

Section: Individual Anthocyanidinsmentioning

confidence: 99%

“…Filtration box specifically refers to ultrafiltration (UF), as described in the M&M. Literature on anthocyanidins in blueberries give varying results in comparison to our study. A contributing factor is the difference in calculating total anthocyanidins by summation of individual anthocyanidin amounts compared to using the pH differential method which calculates total anthocyanidins as one group of compounds based on UV spectra [59,60]. In general, blueberry results reported from a spectrophotometric method are higher in the same sample than results from methods quantifying the individual anthocyanidins [19,30,61].…”

Section: Individual Anthocyanidinsmentioning

confidence: 99%

LC–MS/MS and UPLC–UV Evaluation of Anthocyanins and Anthocyanidins during Rabbiteye Blueberry Juice Processing

et al. 2017

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Blueberry juice processing includes multiple steps and each one affects the chemical composition of the berries, including thermal degradation of anthocyanins. Not-from-concentrate juice was made by heating and enzyme processing blueberries before pressing, followed by ultrafiltration and pasteurization. Using LC-MS/MS, major and minor anthocyanins were identified and semi-quantified at various steps through the process. Ten anthocyanins were identified, including 5 arabinoside and 5 pyrannoside anthocyanins. Three minor anthocyanins were also identified, which apparently have not been previously reported in rabbiteye blueberries. These were delphinidin-3-(p-coumaroyl-glucoside), cyanidin-3-(p-coumaroyl-glucoside), and petunidin-3-(pcoumaroyl-glucoside). Delphinidin-3-(p-coumaroyl-glucoside) significantly increased 50% after pressing. The five known anthocyanidins-cyanidin, delphinidin, malvidin, peonidin, and petunidin-were also quantitated using UPLC-UV. Raw berries and press cake contained the highest anthocyanidin contents and contribute to the value and interest of press cake for use in other food and non-food products. Losses of 75.7% after pressing and 12% after pasteurization were determined for anthocyanidins during not-from-concentrate juice processing.

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“…Total anthocyanin content of the crude extracts were determined by a pH differential method in a 96 well microplate using a spectrophotometer (Versamax microtitre reader) as described by Lee et al (2005) and Lee et al (2008). Results expressed as mg cyanidin-3-glucoside equivalent per g dry plant material were obtained using equations 1 and 2 (Lee et al, 2005).…”

Section: Determination Of Anthocyaninmentioning

confidence: 99%

Polarity of extracts and fractions of four Combretum (Combretaceae) species used to treat infections and gastrointestinal disorders in southern African traditional medicine has a major effect on different relevant in vitro activities

Ahmed

McGaw

Elgorashi

et al. 2014

Journal of Ethnopharmacology

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Graphical abstractPhenolic-enriched extracts of four Combretum species namely Combretum bracteosum, Combretum padoides, Combretum vendae and Combretum woodii used in South African traditional medicine to treat various ranges diseases were evaluated against some diarrhoeal aetiologies. All the crude extracts and their fractions of varied polarities had good antimicrobial (non-polar fractions) or good antioxidant (polar fractions) activity. The crude extract of all the plant species were cytotoxic to Vero cell lines but did not lead to COX enzyme inhibition. Phenolic-enriched extractsFour Combretum species traditionally used for treating infectious and gastrointestinal complications in southern Africa In vitro assays: antioxidant, anti-inflammation, antimicrobial, cytotoxicity AbstractEthnopharmacological importance: Infections and gastrointestinal (GIT) disorders such as diarrhoea causes many problems in human health and animal production. Many Combretum species are used in traditional medicine to treat various diseases by rural people in Africa and Asia. Much of the work done to date on some species to validate their ethnopharmacological use was on the non-polar or intermediate polarity components. Many species are yet to be studied against relevant disease parameters using more polar extracts. Aims:The polar components were extracted and fractionated by solvent-solvent fractionation to yield fractions of different polarities. The activity of these fractions on different parameters that could be involved in infectious and gastrointestinal track (GIT) disorders was investigated. The cytotoxic activities of the extracts were also determined to evaluate the potential of these extracts to combat diarrhoea in production animals. Materials and Methods:Phenolic-enriched leaf extracts of Combretum bracteosum (Cob), Combretum padoides (Cop), Combretum vendae (Cov) and Combretum woodii (Cow) were obtained by extracting with a mixture of 70% acetone acidified with 1% HCl and n-hexane. The extract was sequentially treated by solvent-solvent fractionation with dichloromethane, ethyl acetate, and butanol to yield fractions with a large variation in polarity. The phenolic constituents of the extracts and fractions were determined using standard procedures. The antioxidant activities were determined using various standard methods. The minimum inhibitory concentrations (MICs) of the crude extracts and fractions against four bacterial and three fungal strains were assessed with a microplate serial dilution method.Cyclooxygenase (COX) and lipoxygenase (LOX) enzyme inhibitory assays and cytotoxicity studies against Vero cells were also carried out.Result: Some of the fractions had much higher antioxidant activity than the positive controls. The average EC 50 values of the extracts for the DPPH and ABTS antioxidant assays were 0.21-12 µg/ml (Cop), 0.25-16 µg/ml (Cov), 0.33-9.41 µg/ml (Cow) and 4.97-85 µg/ml (Cob) respectively while the mean EC 50 values for the positive controls ascorbic acid and trolox were 1.28-1.51 and 1.02-1.19 ...

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Correlation of two anthocyanin quantification methods: HPLC and spectrophotometric methods

Cited by 257 publications

References 13 publications

Teores de antocianinas, fenóis totais, taninos e ácido ascórbico em uva 'bordô' sobre diferentes porta-enxertos

Teores de antocianinas, fenóis totais, taninos e ácido ascórbico em uva 'bordô' sobre diferentes porta-enxertos

LC–MS/MS and UPLC–UV Evaluation of Anthocyanins and Anthocyanidins during Rabbiteye Blueberry Juice Processing

Polarity of extracts and fractions of four Combretum (Combretaceae) species used to treat infections and gastrointestinal disorders in southern African traditional medicine has a major effect on different relevant in vitro activities

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