Correlation of Vascular Endothelial Growth Factor Expression and Neovascularization with Colorectal Carcinoma: A Pilot Study

Abstract

Background: In Egypt, there is an increasing incidence of colorectal cancer (CRC), especially among patients under 40 years of age, affecting a very important age group in our community. The basic pathogenic step in the process of tumor growth, invasion and metastasis is tumor induced angiogenesis. The aim of this study was to evaluate the angiogenesis in colorectal carcinoma by detect the expression of VEGF vascular endothelial growth factor and micro vascular density (MVD) determination with IHC (immunohistochemistry) method and to determine if and how angiogenesis correlates with clinicopathologic parameters. Methods and findings:Sixty five archival, paraffin embedded tissue samples of colorectal carcinoma from Pathology Lab of Suez Canal University Teaching Hospital (Egypt) with complete follow-up files in Oncology Unit from. VEGF and micro vessels were identified immunohistochemically, using monoclonal VEGF antibody and CD34 antibody, respectively. VEGF IHC expression was positive in 94.7% of cases and MVD ranged from 9 to 42 mean 24.55 ± 13.79. A significant positive relation was found between VEGF expression; and histological type, tumor grade, lymph node (LN) involvement, UICC TNM classification, perineural and lympho-vascular invasions (p<0.05). A significant positive relation was found between VEGF expression and MVD (p<0.001). There is a significant correlation has been found between MVD and size of the tumor, histological subtype, tumor grade, LN involvement, UICC TNM classification, vascular and perineural invasion and survival (p<0.05). Conclusions:This study has highlighted the prognostic significance of VEGF and MVD to predict survival in CRC patients, as well as some of traditional prognostic factors as tumor histologic type, tumor size, tumor grade, LN involvement, tumor stage and lympho-vascular and perineural invasion to identify patients at high risk for relapse who may benefit from adjuvant treatment including new therapeutic strategies in the future.

Ezh2, endothelin-1, and cd34 as biomarkers of aggressive cervical squamous cell carcinoma: an immunohistochemical study

“…This matches the results of other studies (8,12,27) on cervical and colonic carcinomas. As regards lymph node metastasis, a significant relationship with the high MVD was found.…”

“…This observation was similar to the previous studies (12,25,26) that were done on colorectal carcinoma, breast cancer and oral squamous cell carcinoma respectively.…”

“…Then the highest value among the three fields was obtained for analysis. Any brown stained endothelial cell or clusters of endothelial cells with or without lumen was counted as single micro vessel (12). We calculated the median for 54 cases and used it as a cutoff point.…”

Predictive Factors of Positive Circumferential and Longitudinal Margins in Early T3 Colorectal Cancer Resection

“…However, in a study by Tarta et al [ 38 ], they documented a significant association between tumor high microvessel count and its depth of invasion. In another study by Mohamed et al [ 39 ], they documented a significant correlation between MVD and pathological stage of the tumor and with vascular invasion which has an influence on tumor depth of invasion. Consequently, the depth of invasion has its impact on CRM as discussed earlier.…”

Hubungan Kepadatan Limfosit T Sitotoksik CD8 Dengan Karakteristik Klinikopatologi Adenokarsinoma Kolorektal

“…Sesuai dengan hasil penelitian Lenawi C tahun 2017 yang menyatakan bahwa tidak terdapat hubungan antara kepadatan limfosit dengan ukuran tumor (p-value 1,000) 8 dan penelitian Mohamed dkk tahun 2016, terdapat 35 dari 56 pasien memiliki ukuran tumor ≥ 5 cm (62,5%), serta penelitian Zhi-wei dan Jin tahun 2012, terdapat 176 pasien (51%)dengan ukuran tumor ≥5 cmdari total 345 pasien karsinoma kolorektal. 26,27 Hal ini menunjukkan bahwa banyaknya pasien karsinoma kolorektal yang datang ke rumah sakit dengan ukuran tumor yang sudah besar, karena biasanya penderita kanker kolorektal tidak merasakan gejala atau dengan gejala yang tidak begitu jelas. Kebanyakkan pasienpasien yang terdiagnosis stadium awal karsinoma kolorektal ditemukan tidak sengaja melalui pemeriksaan fisik rutin.…”