Correlation plenoptic imaging for microscopy applications

Scagliola, Alessio; Lena, Francesco Di; Garuccio, Augusto; D’Angelo, Milena; Pepe, F.

doi:10.1016/j.physleta.2020.126472

Cited by 27 publications

(38 citation statements)

References 40 publications

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“…The comparison reported in Table I clarifies the expected theoretical improvements offered by CLM, in terms of both resolution and DOF [36], with respect to both standard microscopy and conventional lightfield microscopy [37]. The first column highlights the…”

Section: Introductionsupporting

confidence: 53%

“…Properties of the light-field microscope are derived by the general features of light-field imaging devices (see [37] for a detailed discussion), while the resolution and DOF limits in CLM are derived from the theoretical analysis reported in Ref. [36], as well as in Materials and Methods.…”

Section: Microscopy Resolutionmentioning

confidence: 99%

“…Here, we design a CPI architecture suitable for different microscopy modalities (fluorescence, polarization, darkfield) that are critical for biological applications. To this end, the sample is illuminated with the whole light beam from a chaotic light source [36], rather than by just one beam out of a correlated beam pair [25,28,29]. This enables imaging self-emitting, scattering and diffusive samples, as well as performing birefringent imaging, without sacrificing the retrieved correlation.…”

Section: Introductionmentioning

confidence: 99%

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Light-field microscopy with correlated beams for extended volumetric imaging at the diffraction limit

Massaro,

Giannella,

Scagliola

et al. 2021

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Light-field microscopy represents a promising solution for microscopic volumetric imaging, thanks to its capability to encode information on multiple planes in a single acquisition. This is achieved through its peculiar simultaneous capture of information on light spatial distribution and propagation direction. However, state-of-the-art light-field microscopes suffer from a detrimental loss of spatial resolution compared to standard microscopes. We propose and experimentally demonstrate a light-field microscopy architecture based on light intensity correlation, in which resolution is limited only by diffraction. We demonstrate the effectiveness of our technique in refocusing threedimensional test targets and biological samples out of the focused plane. We improve the depth of field by a factor 6 with respect to conventional microscopy, at the same resolution, and obtain, from one acquired correlation image, about 130, 000 images, all seen from different perspectives; such multi-perspective images are employed to reconstruct over 40 planes within a 1 mm 3 sample with a diffraction-limited resolution voxel of 20 × 20 × 30 µm 3 .

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Section: Introductionsupporting

confidence: 53%

Section: Microscopy Resolutionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Light-field microscopy with correlated beams for extended volumetric imaging at the diffraction limit

Massaro,

Giannella,

Scagliola

et al. 2021

Preprint

Self Cite

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“…where M and M L are the magnifications of the images of the reference object plane and of the focusing element, respectively, while the power n is equal to 1 or 2, according to whether the object lies in only one [16,33,35] or both [36,37] optical paths. Experimental CPI based on pseudo-thermal light is shown in Figure 2c, where both the acquired out-of-focus image and the corresponding refocused image are shown [16].…”

Section: Plenoptic Imaging With Correlations: From Working Principle To Recent Advancesmentioning

confidence: 99%

“…Our objective is to achieve, in both devices, high resolution, whether diffractionlimited or sub-Rayleigh, combined with a DOF larger by even one order of magnitude compared to standard imaging. The science and technology developed in the project will contribute to establishing a solid baseline of knowledge and skills for the development of a new generation of imaging devices, from quantum digital cameras enhanced by refocusing capability to quantum 3D microscopes [37] and space imaging devices.…”

Section: Perspectivesmentioning

confidence: 99%

Towards Quantum 3D Imaging Devices

et al. 2021

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We review the advancement of the research toward the design and implementation of quantum plenoptic cameras, radically novel 3D imaging devices that exploit both momentum–position entanglement and photon–number correlations to provide the typical refocusing and ultra-fast, scanning-free, 3D imaging capability of plenoptic devices, along with dramatically enhanced performances, unattainable in standard plenoptic cameras: diffraction-limited resolution, large depth of focus, and ultra-low noise. To further increase the volumetric resolution beyond the Rayleigh diffraction limit, and achieve the quantum limit, we are also developing dedicated protocols based on quantum Fisher information. However, for the quantum advantages of the proposed devices to be effective and appealing to end-users, two main challenges need to be tackled. First, due to the large number of frames required for correlation measurements to provide an acceptable signal-to-noise ratio, quantum plenoptic imaging (QPI) would require, if implemented with commercially available high-resolution cameras, acquisition times ranging from tens of seconds to a few minutes. Second, the elaboration of this large amount of data, in order to retrieve 3D images or refocusing 2D images, requires high-performance and time-consuming computation. To address these challenges, we are developing high-resolution single-photon avalanche photodiode (SPAD) arrays and high-performance low-level programming of ultra-fast electronics, combined with compressive sensing and quantum tomography algorithms, with the aim to reduce both the acquisition and the elaboration time by two orders of magnitude. Routes toward exploitation of the QPI devices will also be discussed.

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