Correlation study on expression of GST-π protein in brain tissue and peripheral blood of epilepsy rats induced by pilocarpine

Deng, Xuejun; Jia, Hong; Yang, Zhiyong; Li, Gang; Sun, Shan

doi:10.1007/s11596-011-0586-x

Cited by 5 publications

(2 citation statements)

References 8 publications

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“…Still, expression of GSTP1 was found to be significantly higher in brain specimens from patients with epilepsy, than in controls [11]. Furthermore, Deng et al have found that the change in expression of GSTP in peripheral blood shows the same pattern as that in brain tissues, suggesting GSTP might contribute to drug resistance in epilepsy, suggesting that the GSTP over-expression in peripheral blood could be used as a marker for resistance to anti-epileptic agents [31].…”

Section: Discussionmentioning

confidence: 99%

GSTA1, GSTM1, GSTP1 and GSTT1 polymorphisms in progressive myoclonus epilepsy: A Serbian case–control study

Ercegovac

Jović

Sokić

et al. 2015

Seizure

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Section: Discussionmentioning

confidence: 99%

GSTA1, GSTM1, GSTP1 and GSTT1 polymorphisms in progressive myoclonus epilepsy: A Serbian case–control study

Ercegovac

Jović

Sokić

et al. 2015

Seizure

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“…Based on prior evidence of changes in glutathione‐ S ‐transferase π (GSTP) protein expression levels in the epileptic brain, Deng et al [] measured changes in both brain (by quantitative immunohistochemistry) and blood (by Western blotting) in response to pilocarpine‐induced epilepsy in rats. As hypothesized, levels of GSTP were significantly higher in the drug‐resistant group than in the drug‐responsive group, while no significant difference was found between the control and the drug‐responsive groups.…”

Section: Comparability Of the Proteomementioning

confidence: 99%

On the outside, looking in: A review and evaluation of the comparability of blood and brain “‐omes”

Tylee

Kawaguchi

Glatt

2013

American J of Med Genetics Pt B

210

148

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In this article, we review studies detailing the correspondence between peripheral blood and brain tissue across various domains of high-throughput -omic analysis in order to provide a context for evaluating blood-based biomarker studies. Specifically, we reviewed seven studies comparing patterns of DNA methylation (i.e., an aspect of the epigenome), eight articles comparing patterns of gene expression (i.e., the transcriptome), and three articles comparing patterns of protein expression (i.e., the proteome). Our review of the epigenomic literature suggests that CpG-island methylation levels are generally highly correlated (r ¼ 0.90) between blood and brain. Our review of transcriptomic studies suggests that between 35% and 80% of known transcripts are present in both brain and blood tissue samples; estimates of cross-tissue correlation in expression levels were found to range from 0.25 to 0.64, with stronger correlations observed among particular subsets of genes. Relative to the epigenome and transcriptome, the proteome has not been as fully compared between brain and blood samples, highlighting an important area for future work as whole-proteome profiling methods mature. Beyond reviewing the relevant studies, we discuss some of the assumptions, methodological issues, and gaps in knowledge that should be addressed in order to better understand how the multiple ''-omes'' of the brain are reflected in the peripheral blood. A better understanding of these relationships is a critical precursor to the validation of biomarkers for brain disorders. Ó 2013 Wiley Periodicals, Inc.Key words: blood; brain; epigenome; gene expression; genome; methylation; neuropsychiatry; proteome; transcriptome INTRODUCTIONTechnological advances in molecular biology over the last two decades have fundamentally altered our approach to studying brain disorders. The ''throughput'' of many molecular-profiling techniques, which had heretofore been a major bottleneck to discovery, has increased exponentially. This, in turn, has initiated a notable trend in the field away from hypothesis-testing of candidate genes, transcripts, and proteins, and toward hypothesis-generation through the simultaneous evaluation of all members of a particular molecular species (i.e., an ''-ome''). As a consequence, the number of -omes entering the scientific vernacular has also increased rapidly to include the genome (all DNA sequence variations), epigenome (all chemical modifications to the DNA and histone proteins), transcriptome (all expressed RNA transcripts), and proteome (all expressed proteins), among others.Given the demonstrable heritability of many neuropsychiatric disorders [Glatt et al., 2010], much research has been dedicated to identifying the genomic variations underlying their susceptibility. These genomic studies have been predicated on the fundamental and largely true assumption that the genome is equivalent in sequence and structure in all cells and tissues of the same organism. This tenet allows for the standard practice of examining DNA sequen...

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Effect of adenosine A2A receptor antagonist ZM241385 on amygdala-kindled seizures and progression of amygdala kindling

Liu

et al. 2012

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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The purpose of this study was to evaluate the effect of adenosine A2A receptor antagonist ZM241385 on amygdala-kindled seizures and its roles in epileptogenesis. Electrodes were implanted into the right amygdala of male adult Wistar rats. Kindling was accomplished by using stimulus strength of 500 μA applied daily to the amygdala until 10 consecutive stage 5 seizues were induced. Then effect of ZM241385 was studied in fully kindled rats after intracerebroventricular administration of the drug. In addition, the effect on kindling progression was evaluated through ZM241385 injection before daily stimulation. In all experiments, behavioral changes in the rats in response to ZM241385 were monitored closely. The results showed that, in fully amygdala-kindled rats, ZM241385 (0.001-0.1 nmol/L) decreased afterdischage duration (ADD), motor seizure duration (MSD), stage 5 duration (S5D) and seizure duration (SD), but only the effect on ADD was dose-dependent. The doses of 0.001-0.1 nmol/L had no influence on stage 4 latency (S4L) and seizure stage (SS). The dosages of 0.0001 and 1 nmol/L of ZM241385 did not exert any effect on all seizure parameters. In contrast to the results in fully amygdala-kindled rats, ZM241385 (0.001-0.1 nmol/L) had minimal or no effects on the progression of amygdala-kindled seizures. We are led to the conclusion that although ZM241385 had no influence on the progression of amygdala-kindled seizures, it had potent anti-convulsant profile and little adverse effects at the dosage of 0.001-0.1 nmol/L, suggesting that the agent is effective against the amygdala-kindled seizures.

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Correlation study on expression of GST-π protein in brain tissue and peripheral blood of epilepsy rats induced by pilocarpine

Cited by 5 publications

References 8 publications

GSTA1, GSTM1, GSTP1 and GSTT1 polymorphisms in progressive myoclonus epilepsy: A Serbian case–control study

GSTA1, GSTM1, GSTP1 and GSTT1 polymorphisms in progressive myoclonus epilepsy: A Serbian case–control study

On the outside, looking in: A review and evaluation of the comparability of blood and brain “‐omes”

Effect of adenosine A2A receptor antagonist ZM241385 on amygdala-kindled seizures and progression of amygdala kindling

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