Correlation with OCT and histology of photocoagulation lesions in patients and rabbits

Abstract

ABSTRACT.Purpose: To examine spectral domain optical coherence tomographic (OCT) and histological images from comparable retinal photocoagulation lesions in rabbits, and to correlate these images with comparable OCT images from patients. Methods: 508 rabbit lesions were examined by HE-stained paraffin histology. 1019 rabbit lesions versus 236 patient lesions were examined by OCT, all at the time-points 1 hr, 1 week and 4 weeks after photocoagulation. We analysed 100 lm lesions (in humans) and 133 lm lesions (in rabbits) of 200 ms exposures at powers titrated from the histological threshold up to intense damage. Lesions were matched according to morphological criteria. Results: Dome-shaped layer alterations, retinal infiltration by round, pigmented cells, outer nuclear layer interruption, and eventually full thickness retinal coagulation are detectable in histology and OCT. Horizontal damage extensions are found 1½ times larger in OCT. More intense irradiation was necessary to induce comparable layer affection in rabbit OCT as in histology. Restoration of the inner retinal layers is only shown in the OCT images. Comparable primary lesions caused more pronounced OCT changes in patients than in rabbits during healing. Conclusions: Optical coherence tomographic images indicate different tissue changes than histologic images. After photocoagulation, they show wider horizontal damage diameters, but underestimate axial damage particularly during healing. Conclusions on retinal restoration should not be drawn from OCT findings alone. Retinal recovery after comparable initial lesions appears to be more complete in rabbit than in patient OCTs.

New Frontiers in Selective Retinal Lasers

“…This has been shown to result in both restoration of retina structure and function in these laser spots with intact retinal ganglion cells in the laser region through retinal remodeling [25] . This restoration of morphology has also been shown in humans with OCT following less intense laser burns [26,27] .…”

A Simple Optical Coherence Tomography Quantification Method for Choroidal Neovascularization

“…32 Evaluation of L-CNV in vivo using OCT imaging has been previously studied in different species such as monkeys, 33,34 rabbits, 35 and rats. 27,36,37 Others have evaluated OCT in the mouse model of L-CNV used here by comparing OCT imaging to immunostaining and histological analyses.…”

Comprehensive Detection, Grading, and Growth Behavior Evaluation of Subthreshold and Low Intensity Photocoagulation Lesions by Optical Coherence Tomographic and Infrared Image Analysis

“…An additional halo, which may develop around intense burns, was considered a secondary effect and excluded from the measurements, because it is strongly time-dependent, and exclusion of these halos results in diameter measurements equal to histological damage diameters [27, 28]. In IR images, all reflectivity alterations—bright or dark—that differed from the pretreatment image were included in the lesions size measurement.…”