Correlations among Anaplasma marginale parasitemia and markers of oxidative stress in crossbred calves

De, Ujjwal Kumar; Dey, Sohini; Banerjee, P. S.; Sahoo, Monalisa

doi:10.1007/s11250-011-9938-6

Cited by 23 publications

(15 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results were similar to those reported by others (De et al 2012;Abdel Hamid et al 2014). It was suggested that the severe anaemia could be attributed to immune-mediated destruction of parasitised and non-parasitised erythrocytes (De et al 2012). …”

Section: Discussionsupporting

confidence: 93%

See 1 more Smart Citation

Significance of clinical variables and selected biochemical markers in predicting the outcome of bovine anaplasmosis

El-Ashker¹,

Salama²,

El-Sebaei³

et al. 2015

Vet. Med. - Czech

View full text Add to dashboard Cite

ABSTRACT:The present study was aimed at evaluating the usefulness of selected inflammatory and oxidative stress markers in predicting the clinical outcome of cattle infected with Anaplasma (A). marginale. The study population consisted of 39 cattle naturally infected with A. marginale. The presumptive diagnosis of this infection was initially achieved on the basis of case history, microscopy and clinical examination findings, and confirmed using A. marginale-specific PCR assays. The diseased cattle were categorised according to the clinical outcome into survivors (n = 26) and non-survivors (n = 13). For comparison, ten clinically healthy cattle were randomly selected and served as controls. Blood was drawn from all examined animals to measure the respective levels of selected cytokines, acute phase proteins, oxidative stress markers, and antioxidant enzyme levels. We found that the clinical examination alone was not conclusive and should be used in conjunction with other diagnostic methods. Nonetheless, the non-surviving animals showed anorexia, frequent coughing, dyspnoea, bloody faeces, recumbency, pale or icteric mucous membranes, and haemoglobinuria. Biochemically, tumour necrosis factor alpha, interleukin (IL)-1β, IL-6, serum amyloid A, fibrinogen, malondialdehye, superoxide dismutase, and catalase levels were significantly higher in diseased cattle compared with controls, and were higher in non-survivors than survivors (P < 0.05). In contrast, reduced glutathione (G-SH) was significantly lower in non-surviving cattle than survivors and controls. Interestingly, a significant correlation was found between parasitaemia of the diseased cattle and most of the measured biochemical variables, with IL-1β and G-SH showing the highest correlation. Our findings clearly demonstrate that A. marginale infection is associated with marked inflammatory and oxidative stress responses, which are higher in non-surviving cattle compared with survivors. The overall degree of cytokine and anti-oxidative disruption may have an important prognostic value for the disease outcome.

show abstract

Section: Discussionsupporting

confidence: 93%

“…Nonetheless, the efficiency of traditional microscopic examination of Giemsa-stained blood films in detecting carrier animals with low rates of parasitaemia is very limited (Younis et al 2009). The clinical findings presented in this study were in part similar to those previously described (De et al 2012;OIE 2012;El-Ashker et al 2015).…”

Section: Discussionsupporting

confidence: 90%

Significance of clinical variables and selected biochemical markers in predicting the outcome of bovine anaplasmosis

El-Ashker¹,

Salama²,

El-Sebaei³

et al. 2015

Vet. Med. - Czech

View full text Add to dashboard Cite

show abstract

“…The infection leads to a drastic increase in the number of infected erythrocytes. These cells are then phagocytosed by reticuloendothelial cells leading to hemolytic anemia and icterus [ 7 ]. Clinical signs of A. marginale infection include anorexia, dyspnea, tachycardia, hemolytic anemia, jaundice, lacrimation, salivation, fever, fatigue, diarrhea, frequent urination, and abortion.…”

Section: Introductionmentioning

confidence: 99%

“…Changes in indices of oxidative stress are recorded in parasitic diseases. The role of erythrocytic peroxidation in the pathogenesis of several hemiparasitic infections is well recognized [ 7 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular diagnosis and biochemical studies of tick-borne diseases (anaplasmosis and babesiosis) in Aberdeen Angus Cattle in New Valley, Egypt

et al. 2020

View full text Add to dashboard Cite

Background and Aim: Anaplasmosis and babesiosis are tick-borne diseases that threaten livestock production with subsequent considerable economic losses. This study was conducted to diagnose Anaplasma and Babesia infection using molecular techniques in imported Aberdeen Angus cattle imported from Uruguay to El-Kharga Oasis in New Valley, Egypt, and to investigate the effects of disease on some serum biochemical and oxidative stress parameters. Materials and Methods: Blood samples were collected from 31 cattle, 21 diseased and ten apparently normal, of varying ages and sex. The blood was used for the preparation of blood smears, polymerase chain reaction assay, and separation of serum for biochemical investigation. The experimental production farm at the Faculty of Agriculture, New Valley University, was infested with ticks and variable clinical manifestations during the period from December 2017 to March 2018. One calf died of a suspected blood parasite infection. Results: The blood film examination revealed infection by blood parasites in 21 samples. Anaplasma marginale and Babesia bovis were identified in 12 and 14 samples, respectively. A total of 14 samples were examined by polymerase chain reaction (PCR) to make these identifications. Biochemical parameters showed significantly elevated serum alanine aminotransferase, aspartate aminotransferase, total bilirubin (T. Bil), and urea in blood from parasite-infected female cattle and male calves compared with controls. Increased serum total protein, globulin, and creatinine were recorded only in infected female cattle. The blood glucose level was significantly decreased in infected female cattle and male calves compared with controls. Furthermore, albumin and albumin/globulin ratio was significantly reduced in the infected female cattle. Oxidative stress profiles of infected animals showed a significant increase in serum nitric oxide and malondialdehyde, and both total antioxidant capacity and reduced glutathione (GSH) were significantly reduced in comparison with control animals. Conclusion: The incidence of A. marginale and B. bovis infection is high in imported Aberdeen Angus cattle in New Valley Province. PCR methods provide a short-term assessment of disease. An extensive epidemiological survey, employing serology together with molecular genetic methods, monitoring of abundance and distribution of tick vectors, availability of vaccination programs, and tracking of animal transport is also needed for control of blood parasites.

show abstract

“…After infection, parasitemia increases until the hemolytic crisis, frequently with more than 50% of RBCs infected 18 . The number of infected erythrocytes increases drastically and phagocytosis by reticulo-endothelial cells of parasitized erythrocytes lead to development of hemolytic anemia and icterus 19 . Serum biochemical parameters such as aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT), concentrations are indicators of hepatic function.…”

Section: Introductionmentioning

confidence: 99%

Acute Phase Proteins, Clinical, Hematological and Biochemical Parameters in Dairy Cows Naturally Infected with Anaplasma Marginale

Coşkun¹,

Ekici²,

Güzelbekteş³

et al. 2012

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

SummaryThe aim of the study was to evaluate acute phase response via Haptoglobin and serum amyloid-A concentrations in dairy cows naturally infected with Anaplasma marginale. The second aim of the study was to determine the changes in clinical, hematological and biochemical parameters in dairy cows naturally infected Anaplasma marginale. A total of 40 dairy cattle suffering from bovine anaplasmosis were included to the study from a dairy cattle herd. A total of 10 healthy dairy cattle were selected for control group. Analysis of acute phase proteins, hematologic analysis and biochemical analysis was performed in this study. Serum haptoglobin and serum amyloid-A concentrations significantly increased in cattle infected with Anaplasma marginale compared to healthy cattle. All cattle in infected group demonstrated clinical signs of anaplasmosis. Significantly decreased red blood cell count, packed cell volume, and hemoglobin concentration were observed in infected cattle compared to the control group. Serum aspartate aminotransferase, alkaline phosphatase, creatinine and bilirubin concentrations were significantly increased in infected cattle compared with the control group. In conclusion, the changes of biochemical and hematological parameters may be indicate of anemia and tissue damage in cattle with anaplasmosis. Serum haptoglobin and serum amyloid-A concentrations could be usefull in evaluate of acute phase response in cattle infected with Anaplasma marginale.

show abstract

Correlations among Anaplasma marginale parasitemia and markers of oxidative stress in crossbred calves

Cited by 23 publications

References 29 publications

Significance of clinical variables and selected biochemical markers in predicting the outcome of bovine anaplasmosis

Significance of clinical variables and selected biochemical markers in predicting the outcome of bovine anaplasmosis

Molecular diagnosis and biochemical studies of tick-borne diseases (anaplasmosis and babesiosis) in Aberdeen Angus Cattle in New Valley, Egypt

Acute Phase Proteins, Clinical, Hematological and Biochemical Parameters in Dairy Cows Naturally Infected with Anaplasma Marginale

Contact Info

Product

Resources

About