Correlations between hematopoietic progenitor cell counts as measured by Sysmex and CD34+ cell harvest yields following mobilization with different regimens

Vogel, Wichard; Hg, Kopp; Kanz, Lothar; Einsele, Hermann

doi:10.1007/s00432-002-0351-4

Cited by 18 publications

(5 citation statements)

References 15 publications

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“…It is interesting that the HPC method correlated well with the standard CD34 + cell determination in the peripheral blood but the HPC count agreed only weakly with the reference method in the collection product ( R = 0.464) and similar results have been reported by Vogel et al [28]. Our group of patients was relatively small and it is possible that the correlation index could increase if more patients are used.…”

Section: Discussionsupporting

confidence: 86%

Timing of Peripheral Blood Stem Cell Yield: Comparison of Alternative Methods with the Classic Method for CD34⁺Cell Determination

Fatorova

Bláha

Lánska

et al. 2014

BioMed Research International

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Hematopoietic stem cells (HSCs), still represent a certain mystery in biology, have a unique property of dividing into equal cells and repopulating the hematopoietic tissue. This potential enables their use in transplantation treatments. The quality of the HSC grafts for transplantation is evaluated by flow cytometric determination of the CD34+ cells, which enables optimal timing of the first apheresis and the acquisition of maximal yield of the peripheral blood stem cells (PBSCs). To identify a more efficient method for evaluating CD34+ cells, we compared the following alternative methods with the reference method: hematopoietic progenitor cells (HPC) enumeration (using the Sysmex XE-2100 analyser), detection of CD133+ cells, and quantification of aldehyde dehydrogenase activity in the PBSCs. 266 aphereses (84 patients) were evaluated. In the preapheretic blood, the new methods produced data that were in agreement with the reference method. The ROC curves have shown that for the first-day apheresis target, the optimal predictive cut-off value was 0.032 cells/mL for the HPC method (sensitivity 73.4%, specificity 69.3%). HPC method exhibited a definite practical superiority as compared to other methods tested. HPC enumeration could serve as a supplementary method for the optimal timing of the first apheresis; it is simple, rapid, and cheap.

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Section: Discussionsupporting

confidence: 86%

Timing of Peripheral Blood Stem Cell Yield: Comparison of Alternative Methods with the Classic Method for CD34⁺Cell Determination

Fatorova

Bláha

Lánska

et al. 2014

BioMed Research International

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“…Previous comparisons of HPC analysis by Sysmex SE and XE series analyzers with CD34+ cell counting showed only moderate correlations, 19,20 and the relationship between preharvest peripheral blood HPC counts and CD34+ yields in apheresis products was not strong. 31 Although high peripheral blood HPC counts were useful for determining when to initiate stem cell collection, low HPC counts (<5 × 10 6 /L) often underestimated peripheral blood CD34+ cells, and thus CD34 analysis was required.…”

Section: Discussionmentioning

confidence: 78%

“…17-19 Although HPC appears to be a useful positive predictor of when to initiate apheresis to obtain desired CD34+ cell yields, HPC levels below predefined cutoffs have not reliably predicted poor CD34+ cell collections. The latter has limited the use of HPC as a surrogate for CD34 analysis in PBSCT.…”

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confidence: 99%

Evaluation of new automated hematopoietic progenitor cell analysis in the clinical management of peripheral blood stem cell collections

et al. 2015

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BACKGROUND Successful peripheral blood stem cell transplantation (PBSCT) depends on the collection and infusion of adequate numbers of peripheral blood progenitor cells (PBPCs). Several predictors of PBPC yield are used currently, including white blood cell (WBC) count and CD34 analysis. This study evaluated the utility of the new automated hematopoietic progenitor cell count available on Sysmex XN hematology analyzers (XN-HPCs) in PBSCT. STUDY DESIGN AND METHODS The performance characteristics of XN-HPC, CD34+, and WBC analysis were compared using 107 matched peripheral blood and apheresis samples. RESULTS Good correlation was observed between XN-HPC and CD34+ cell counts in peripheral blood (r = 0.88; slope, 0.81) and apheresis collections (r = 0.91; slope, 0.89). Moreover, peripheral blood XN-HPC and CD34 analysis showed comparable ability to predict successful PBPC harvests (≥ 2 × 106 CD34+ cells/kg). At a cutoff of 20 × 106 progenitor cells/L, peripheral blood XN- HPC and CD34 analysis both showed negative predictive values (NPVs) of 100% and positive predictive values (PPVs) of 55.4 and 63%, respectively. Using an optimized cutoff of 38 × 106 progenitor cells/L, derived from receiver operating characteristic analysis, the PPV for XN-HPC and CD34 analysis increased to 71.4 and 78.9%, respectively, with relatively unchanged NPVs (XN-HPC 97.7%, CD34+ 98.0%). In contrast, the correlation between peripheral blood WBC and CD34 analysis was poor (r = 0.48; slope, 669.85), and the peripheral blood WBC count (cutoff, 10 × 109/L) was a poor predictor of PBPC harvest (NPV 60%, PPV 43.1%). CONCLUSION XN-HPC compares favorably with CD34 analysis and may be a surrogate for CD34 analysis to predict optimal timing of PBPC collections.

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“…The HPC count can be rapidly, simply and cost-effectively obtained by using the IMI channel of the automated hematology analyzer. However, the correlation of the HPC count in the peripheral blood with the number of CD34+ cells in the peripheral blood or in the apheresis yields has differed among reports (r = 0.556–0.88 and 0.62–0.762, respectively) [8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18]. The discrepancy might have resulted from differences in the conditions of storage of blood samples, which is not described in great detail in these reports.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, we and others have reported that the HPC count monitored by an immature information (IMI) channel of the SE-9000 or SE-9500 hematology analyzer (TOA Medical Electronica, Kobe, Japan) is an alternative indicator of the optimal timing for apheresis procedures [8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18]. The HPC count can be rapidly, simply and cost-effectively obtained by using the IMI channel of the automated hematology analyzer.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Optimal Blood Sampling Conditions for Estimation of Hematopoietic Progenitor Cell Count by the SE-9000 Automated Hematolyzer

Nomura

Kubota²,

Baba³

et al. 2004

Acta Haematol

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We evaluated the optimal conditions for blood sampling for hematopoietic progenitor cells (HPCs) as estimated by the immature information program of the SE-9000 automated hematology analyzer. The HPC count was most stable when the blood samples were incubated at room temperature with ethylene-diaminetetraacetic acid dipotassium (EDTA-2K) as an anticoagulant. The HPC count should, however, be measured within 4 h after blood collection, even under optimal conditions. In contrast, the CD34+ cell count estimated by flow cytometric analysis was stable for at least 21 h after the blood samples were incubated with EDTA-2K at room temperature or 4°C. When appropriate blood samples were used, the HPC count in the peripheral blood significantly correlated with the CD34+ cell count in the peripheral blood and in the apheresis yields (r = 0.798 and 0.635, respectively); therefore, the HPC count is a reliable predictor for initiation of apheresis procedures to obtain sufficient HPCs for peripheral blood stem cell transplantation.

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Correlations between hematopoietic progenitor cell counts as measured by Sysmex and CD34+ cell harvest yields following mobilization with different regimens

Abstract: Lymphoma and multiple myeloma patients who were moderately pretreated and mobilized with chemotherapy and G-CSF showed the best results in correlation analyses even at low HPC counts. Therefore, HPC measurement can be used for timing of apheresis in these patients.

Cited by 18 publications

References 15 publications

Timing of Peripheral Blood Stem Cell Yield: Comparison of Alternative Methods with the Classic Method for CD34⁺Cell Determination

Timing of Peripheral Blood Stem Cell Yield: Comparison of Alternative Methods with the Classic Method for CD34⁺Cell Determination

Evaluation of new automated hematopoietic progenitor cell analysis in the clinical management of peripheral blood stem cell collections

Analysis of the Optimal Blood Sampling Conditions for Estimation of Hematopoietic Progenitor Cell Count by the SE-9000 Automated Hematolyzer

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Correlations between hematopoietic progenitor cell counts as measured by Sysmex and CD34+ cell harvest yields following mobilization with different regimens

Abstract: Lymphoma and multiple myeloma patients who were moderately pretreated and mobilized with chemotherapy and G-CSF showed the best results in correlation analyses even at low HPC counts. Therefore, HPC measurement can be used for timing of apheresis in these patients.

Cited by 18 publications

References 15 publications

Timing of Peripheral Blood Stem Cell Yield: Comparison of Alternative Methods with the Classic Method for CD34+Cell Determination

Timing of Peripheral Blood Stem Cell Yield: Comparison of Alternative Methods with the Classic Method for CD34+Cell Determination

Evaluation of new automated hematopoietic progenitor cell analysis in the clinical management of peripheral blood stem cell collections

Analysis of the Optimal Blood Sampling Conditions for Estimation of Hematopoietic Progenitor Cell Count by the SE-9000 Automated Hematolyzer

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Product

Resources

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Timing of Peripheral Blood Stem Cell Yield: Comparison of Alternative Methods with the Classic Method for CD34⁺Cell Determination

Timing of Peripheral Blood Stem Cell Yield: Comparison of Alternative Methods with the Classic Method for CD34⁺Cell Determination