Correlations between human salivary levels of lysozyme, lactoferrin, salivary peroxidase and secretory immunoglobulin A with different stimulatory states and over time

Rudney, Joel D.; Kajander, Keith C.; Smith, Q. T.

doi:10.1016/0003-9969(85)90129-3

Cited by 38 publications

(19 citation statements)

References 32 publications

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“…These were 7-12 years old in the study of Cole et al [1981] and 16 years of age when samples were taken in this trial. Nonetheless, results obtained here are also in good agreement with the values reported by Rudney et al [1985]. No significant correlations were found between levels of any of the antimicrobial agents and caries increments, caries score or gingival index.…”

supporting

confidence: 92%

Concentration of Antimicrobial Proteins in Human Saliva

1990

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supporting

confidence: 92%

Concentration of Antimicrobial Proteins in Human Saliva

1990

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“…The concentration of lactoferrin in unstimulated saliva is about 7 to 20 g/ml (126,371), and its fungicidal activity against C. albicans (404) has been attributed not only to sequestration of ferrous ions (284) but also to structural damage to the fungal cell wall (313) and activation of intracellular autolytic enzymes (243). Salivary concentrations of lactoferrin in patients with HIV infection have been variously reported to be increased (20,258), unchanged (276), or decreased (266,300).…”

Section: Alterations In Mechanisms Of Oral Innate Resistance To C Almentioning

confidence: 99%

Immunopathogenesis of Oropharyngeal Candidiasis in Human Immunodeficiency Virus Infection

2004

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Oropharyngeal and esophageal candidiases remain significant causes of morbidity in human immunodeficiency virus (HIV)-infected patients, despite the dramatic ability of antiretroviral therapy to reconstitute immunity. Notable advances have been achieved in understanding, at the molecular level, the relationships between the progression of HIV infection, the acquisition, maintenance, and clonality of oral candidal populations, and the emergence of antifungal resistance. However, the critical immunological defects which are responsible for the onset and maintenance of mucosal candidiasis in patients with HIV infection have not been elucidated. The devastating impact of HIV infection on mucosal Langerhans' cell and CD4+ cell populations is most probably central to the pathogenesis of mucosal candidiasis in HIV-infected patients. However, these defects may be partly compensated by preserved host defense mechanisms (calprotectin, keratinocytes, CD8+ T cells, and phagocytes) which, individually or together, may limit Candida albicans proliferation to the superficial mucosa. The availability of CD4C/HIV transgenic mice expressing HIV-1 in immune cells has provided the opportunity to devise a novel model of mucosal candidiasis that closely mimics the clinical and pathological features of candidal infection in human HIV infection. These transgenic mice allow, for the first time, a precise cause-and-effect analysis of the immunopathogenesis of mucosal candidiasis in HIV infection under controlled conditions in a small laboratory animal

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“…Another potential reason lies in the different concentrations of non-specific proteins in plasma and saliva. Albumin concentrations in_serum, for example, exceed 4000 mg/dl [20], whereas only concentrations below 200 mg/dl were found in saliva [21,22]. Therefore there is almost'no competition in saliva samples between the highly specific antibody and the non-specific albumin.…”

Section: Discussionmentioning

confidence: 99%

Measurement of Cortisol in Saliva Using a Commercial Radioimmunoassay Developed for Serum. Messung von Speichel-Cortisol mittels eines kommerziellen Serum-Radioimmunoassays

Gröschl¹,

Biskupek-Sigwart²,

Rauh³

et al. 2000

LaboratoriumsMedizin

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A commercially available radioimmunoassay developed for the determination of coriisol in human plasma and serum was used to measure salivary cortisol. Clearly elevated levels for spiked saliva (3-10 fold: P/B regression: y = 1.9x + 6.4; r = 0.983) were found. We suggest that differing biochemical conditions in the standard material (plasma) and the sample matrix (saliva) were responsible for the elevated measurements. For this reason we adapted the assay by replacing the original plasma standards by in-house saliva standards. In addition the concentrations of the antibodies and the tracer were decreased and the time for incubation was increased. After adaptation, cortisol could be measured in excellent accordance with the amounts added to the saliva samples (P/B regression: y = 1.03x -0.01; r = 0.998). The 50% intercept was 8 ng/ml. We measured intra-assay variation coefficients of 7.9% (1 ng/ml) and 6.1% (10 ng/ml), respectively. Inter-assay variation coefficients were 11.2% (1 ng/ml) and 9.6% (10 ng/ml), respectively. Saliva samples of healthy volunteers measured after adaptation were in good accordance with data from in-house assays reported in the literature. There was no disturbing influence of either dental care or food intake on the reproducibility of salivary cortisol levels by this specific assay (p=0.76 and p>0.05, respectively). After adaptation, the assay is therefore a reliable tool for salivary cortisol measurement. · Zusammenfassung: Ein kommerziell erhältlicher Radioimmunoassay zur Messung von Cortisol in humanem Plasma und Serum wurde auf seine Verwendbarkeit zur Messung von Cortisol im Speichel über-prüft. Es ergaben sich 3-10fach erhöhte Werte in Speichelproben, welche mit exogenem Cortisol versetzt wurden (P/B Regression: y = l,9x + 6,4; r = 0,983). Wir nehmen an, daß die unterschiedliche biochemische Zusammensetzung des Standardmaterials (Plasma) und der Probenmatrix (Speichel) der Grund.für die falsch-hohen Messungen ist. Der Assay wurde fol-

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Correlations between human salivary levels of lysozyme, lactoferrin, salivary peroxidase and secretory immunoglobulin A with different stimulatory states and over time

Cited by 38 publications

References 32 publications

Concentration of Antimicrobial Proteins in Human Saliva

Concentration of Antimicrobial Proteins in Human Saliva

Immunopathogenesis of Oropharyngeal Candidiasis in Human Immunodeficiency Virus Infection

Measurement of Cortisol in Saliva Using a Commercial Radioimmunoassay Developed for Serum. Messung von Speichel-Cortisol mittels eines kommerziellen Serum-Radioimmunoassays

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