Correlations of MMP‐2 and MMP‐9 gene polymorphisms with the risk of hepatopulmonary syndrome in cirrhotic patients: A case‐control study

Abstract

Hepatopulmonary syndrome (HPS) increases the mortality of patients who suffered from liver cirrhosis, especially patients plagued by severe hypoxemia. Gene polymorphisms are reported to be related to the risk of HPS in cirrhotic patients. Thus, our study aims to elucidate the correlation between MMP‐2 and MMP‐9 gene polymorphisms and HPS in cirrhotic patients. A total of 152 cirrhotic patients suffering from HPS as well as another 152 cirrhotic patients without HPS were recruited. Polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) was performed for MMP‐2 and MMP‐9 gene polymorphisms and logistic regression analysis for the relationship between clinicopathological features and HPS occurrence in cirrhotic patients. There were significant differences in genotype and allele frequency of MMP‐2 rs243865 and MMP‐9 rs3918242 polymorphisms between the HPS and control groups. CC/CT genotype and C allele of MMP‐2 rs243865 polymorphism as well as CC/TT genotype and T allele of MMP‐9 rs3918242 polymorphism increased the risk of HPS in cirrhotic patients. Genotypes of rs243865 and rs3918242 polymorphisms had remarkable correlations with spider nevi, clubbed fingers (toes), transaminase elevation, portal vein width, esophageal varices, Child‐Pugh classification and partial pressure of arterial oxygen (PaO2). Logistic regression analysis showed that rs243865 and rs3918242 polymorphisms, spider nevi, clubbed fingers (toes), esophageal varices, and Child‐Pugh classification were closely associated with the occurrence of HPS in cirrhotic patients. Our findings demonstrate that MMP‐2 rs243865 polymorphism and MMP‐9 rs3918242 polymorphism can increase the risk of HPS occurrence in cirrhotic patients, which provides a potential target for prevention of HPS in cirrhotic patients.

Genetic polymorphism of matrix metalloproteinase 9 and susceptibility to chronic obstructive pulmonary disease: A meta-analysis

“…Many studies have reported the involvement of MMP9 in the development of lung diseases [29] . MMP9 polymorphism is identified to increase the susceptibility to respiratory diseases [30] [31] [32] [33] . Multiple SNPs of MMP9 have been discovered.…”

Plasma TIMP-1 is a Useful Tool for Monitoring 24 Months Liver Fibrosis Improvement After Anti-HCV /DAA Therapy in HCV/HIV Mono/Coinfection

“…Interestingly this MMP-2 rs 243865 SNP has been associated with central obesity and non-alcoholic fat liver disease and with increased risk of cirrhotic hepatopulmonary syndrome in Chinese patients. However the carriage of the variant TT genotype of this MMP-2 SNP decreased the risk for both hepatic complications [42,43].…”

“…MMPs activity is regulated at gene expression level and by activation of latent pro-MMPs to active MMPs [16,17]. MMPs polymorphisms (SNPs) might modify gene expression and MMPs plasma levels, and indirectly in uence LF [27][28][29][30][31]. MMPs plasma levels are higher in HIV-infected naïve compared to HCV monoinfected patients [32][33][34], while effective antiretroviral therapy normalizes their plasma MMPs levels [32].…”