Correlative light and electron microscopy of wall formation in Eimeria nieschulzi

Wiedmer, Stefanie; Kurth, Thomas; Buder, Ulrike; Bleischwitz, Sinja; Entzeroth, Rolf; Kurth, Michael

doi:10.1007/s00436-020-06765-6

Cited by 9 publications

(14 citation statements)

References 57 publications

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“…The oocyst wall consists mainly of proteins and lipids 39 . Polysaccharide granules and lipid droplets are also found in the cytoplasm of mature macrogamonts in T. gondii and E. maxima 39 , 94 , 95 . Recent studies indicate that the coccidian oocyst wall architecture is comprised not only of glycoproteins but also of an outer layer of acid-fast lipids 39 .…”

Section: Resultsmentioning

confidence: 98%

The transcriptome from asexual to sexual in vitro development of Cystoisospora suis (Apicomplexa: Coccidia)

Cruz‐Bustos

Feix

Lyrakis

et al. 2022

Sci Rep

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The apicomplexan parasite Cystoisospora suis is an enteropathogen of suckling piglets with woldwide distribution. As with all coccidian parasites, its lifecycle is characterized by asexual multiplication followed by sexual development with two morphologically distinct cell types that presumably fuse to form a zygote from which the oocyst arises. However, knowledge of the sexual development of C. suis is still limited. To complement previous in vitro studies, we analysed transcriptional profiles at three different time points of development (corresponding to asexual, immature and mature sexual stages) in vitro via RNASeq. Overall, transcription of genes encoding proteins with important roles in gametes biology, oocyst wall biosynthesis, DNA replication and axonema formation as well as proteins with important roles in merozoite biology was identified. A homologue of an oocyst wall tyrosine rich protein of Toxoplasma gondii was expressed in macrogametes and oocysts of C. suis. We evaluated inhibition of sexual development in a host-free culture for C. suis by antiserum specific to this protein to evaluate whether it could be exploited as a candidate for control strategies against C. suis. Based on these data, targets can be defined for future strategies to interrupt parasite transmission during sexual development.

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Section: Resultsmentioning

confidence: 98%

The transcriptome from asexual to sexual in vitro development of Cystoisospora suis (Apicomplexa: Coccidia)

Cruz‐Bustos

Feix

Lyrakis

et al. 2022

Sci Rep

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“…Immuno uorescence with anti-GAM22 and anti-GAM59 as markers revealed that macrogametocyte development and oocyst wall formation in E. necatrix was similar to those of E. maxima [10,17], E. tenella [17,18], E. acervuline [17] and E. nieschulzi [19], revealing that the structure of macrogametocytes and formation of the oocyst wall are strongly conserved across Eimeria species. Further observations using SEM showed that WFB1 localized to the surface of macrogametocytes appeared as dense spherical granules with a relatively smooth surface.…”

Section: Discussionmentioning

confidence: 90%

“…Previous studies have shown that WFB2 characteristically reacts with anti-GAM56, anti-GAM82 and anti-GAM230 pAbs, with anti-GAM56 and anti-GAM82 pAbs appearing to react more strongly with the whorled or "doughnut-shaped" structures that form groups within WFB2 and anti-GAM230 pAb appearing to stain the material around the cores [10,17,19]. EnGAM59 protein, a second GAM56-like protein similar to GAM56 and GAM82 of other chicken Eimeria, contains a tyrosine-rich domain [20,22].…”

Section: Discussionmentioning

confidence: 99%

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Examination of gametocyte protein 22 localization and oocyst wall formation in Eimeria necatrix using laser confocal microscopy and scanning electron microscopy

Wang

Liu

Gao

et al. 2023

Preprint

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Background Eimeria parasite infection occurs via ingestion of oocysts. The robust, bilayered oocyst wall is formed from the contents of wall forming bodies (WFBs), WFB1 and WFB2, located exclusively in macrogametocytes. Eimeria necatrix gametocyte proteins 22 and 59 (EnGAM22 and EnGAM59) have been found to localize to WFBs and the oocyst wall. However, the exact localization of these two proteins is not clear. The mechanisms of macrogametogenesis and oocyst wall formation in E. necatrix are also unknown. Methods WFBs of E. necatrix were extracted from purified gametocytes using a cut-off filter and observed by negative stain electron microscopy to assess the intactness of the WFBs. The extracts of purified WFBs and gametocytes were analyzed using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. Then, the localization of EnGAM22 and EnGAM59 proteins was determined using an indirect immunofluorescence assay. Finally, the development of macrogametocytes and the oocyst wall of E. necatrix was analyzed using laser confocal microscopy and scanning electron microscopy. Results Purified WFBs had the same shape and size as those observed in macrogametocytes. EnGAM22 protein localized to WFB1, whereas EnGAM59 protein localized to WFB2. Both EnGAM22 and EnGAM59 native proteins were detected in the extracts of WFBs and gametocytes. The outer layer of the oocyst wall was formed by the release of the contents of WFB1 at the surface of the macrogametocyte to form an anti-EnGAM22 positive layer. WFB2 then appeared to give rise to the inner layer, which was anti-EnGAM59 positive. Conclusions EnGAM22 and EnGAM59 proteins localized to WFB1 and WFB2 and were involved in the formation of the outer and inner layers of the oocyst wall of E. necatrix, respectively. The processes of macrogametogenesis and oocyst wall formation of E. necatrix are similar to other Eimeria parasites. The anti-EnGAM22 antibody could be used as a tool to track the transport and secretion of proteins in WFB1 during oocyst development.

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“…One of them, genus Eimeria is a monoxenous coccidian, primarily infecting a single host in their life cycle (Girard et al, 2016). Eimeria is also known as obligate intracellular parasites that have significant roles in medical or veterinary importance (Wiedmer et al, 2020). Species of the genus Eimeria destroy the epithelial cells lining the intestines and also lead to inflammation, bleeding, and bloody diarrhea in case of severe infections (Oldham, 1967).…”

Section: Discussion (I) Protozoan Parasitesmentioning

confidence: 99%

A Survey of Ecto and Endo-Parasites of House Mouse Mus Musculus Linnaeus, 1758 of Erbil City, Kurdistan Region, Iraq

Malla

Saida

2022

Bull. Iraq nat. Hist. Mus.

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In the present survey 18 species of endo and ecto-parasites were recorded during the examination of 50 Mus musculus (Linnaeus, 1758) among 10 localities in Erbil city, of which 7 species were protozoan and as follows : Chilomastix bettencourti (da Fonseca 1915)82%; Giardia muris (Filice, 1952) 68%; Tritrichomonas muris (Grassi,1879)36%; Entamoeba histolytica (Schaudinn,1903) 24%; Entamoeba coli (Grassi,1879)32%; Eimeria sp. 28% and Trypanosoma musculi (Kendall,1906) 2%; and 8 species were helminthes as follows: 4 Cestodes: Rodentolepis nana (von Siebold, 1852) 8%; Hymenolepis diminuta (Rudolphi, 1819)2%; larval stage of Echinococcus granulosus (Batsch, 1786)8%, Cysticercus fasciolaris (Rudolphi, 1808)6%, 4 Nematodes: Aspiculuris tetraptera (Nitzsch, 1821)8%; Syphacia obvelata (Rudolphi, 1802)36%; Syphacia muris (Yamaguti, 1935)2% and Trichuris muris (Schrank, 1788)10%; and 3 species of ectoparasites were diagnosed as follows: the Oriental rat flea Xenopsylla cheopis (Rothschild, 1903)2.0%, the spined rat louse Polyplax spinulosa (Burmeister, 1839)16.0%, and the mite Laelaps nuttalli (Hirst, 1916)4.0%. Endo-multiple infections had been noticed, as single (26%); double 50.0%; triple (22.0%) and tetra infections 2.0%. No significant differences were found between the sexes and weights of mice. The mice of Hayaskary and Langa were high infected with parasites. In the current study, we recorded the infection of the liver of Mus musculus with larval stage of Echinococcus granulosus (hydatid cyst) as the first natural infection in Iraq.

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Correlative light and electron microscopy of wall formation in Eimeria nieschulzi

Cited by 9 publications

References 57 publications

The transcriptome from asexual to sexual in vitro development of Cystoisospora suis (Apicomplexa: Coccidia)

The transcriptome from asexual to sexual in vitro development of Cystoisospora suis (Apicomplexa: Coccidia)

Examination of gametocyte protein 22 localization and oocyst wall formation in Eimeria necatrix using laser confocal microscopy and scanning electron microscopy

A Survey of Ecto and Endo-Parasites of House Mouse Mus Musculus Linnaeus, 1758 of Erbil City, Kurdistan Region, Iraq

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