Corresponding electrode processes of nucleic acids, proteins and nucleoproteins

Berg, Hermann; Fiedler, Ursula; Flemming, J.; Horn, G.

doi:10.1016/0302-4598(85)80013-1

Cited by 10 publications

(4 citation statements)

References 14 publications

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“…The four curves (1−4) in Figure show the effect of adding increasing concentrations of high-molecular-weight DNA to the aqueous solution. As shown by previous workers, − the effective diffusion coefficient of small molecules such as MV 2+ is dramatically reduced as a result of binding to DNA. The diffusion-limited plateau current decreases accordingly, and this information can be used to extract the binding constant after calculating the mole fraction of bound cation from eq 7.…”

Section: Resultssupporting

confidence: 53%

“…The binding of cations to DNA is a field of current interest owing to the importance of these interactions in determining nucleic acid conformation, − their use as structural probes of DNA, − and their use in hybridization sensors in which DNA duplexes are detected by the binding of metal complexes. , An example is the detection of short DNA sequences of the human immunodeficiency virus type 1 (HIV-1) using trisphenanthrolinecobalt(III) . Several techniques have been employed to study the binding of small molecules to DNA (and other polyelectrolytes) including, for example, viscometry, − UV/visible spectroscopy, , luminescence, − electrophoresis, NMR, , and electroanalytical techniques. − Electrochemical techniques have been reported to have several advantages in these measurements, e.g., applicability over a wider range of binding constants than classical methods such as dialysis and to many metal complexes that do not show large changes in molar absorptivity on binding to DNA . The electroanalytical techniques usually rely on the effect of the macromolecule (e.g., DNA) on the diffusion current of a metal complex.…”

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confidence: 99%

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Cation Binding to DNA Studied by Ion-Transfer Voltammetry at Micropipets

Horrocks

Mirkin

1998

Anal. Chem.

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Two new approaches to the study of ion binding to DNA have been developed. Both are based on measurement of ion transfer across the interface between two immiscible electrolyte solutions. In the first method, the cation of interest is initially present in the aqueous phase and transferred to the organic phase contained in a micropipet when its potential is made sufficiently negative. Upon addition of high-molecular-weight DNA to the aqueous phase, the concentration of free cation decreases, which results in a decrease in the ion-transfer current. The corresponding binding constant can be extracted from the dependence of normalized steady-state current vs DNA concentration without the knowledge of micropipet size, binding kinetics, or diffusion coefficient values. In the second method, the cation of interest is present in the organic phase inside the pipet and oligonucleotides (fragments of DNA) are added to the external aqueous phase. The transfer of the cation to the aqueous phase may be facilitated by the oligonucleotides present in the aqueous phase. The facilitated transfer appears as a steady-state wave dependent on the concentration of oligonucleotides in the aqueous phase. The binding constant can be estimated from the shift in the transfer potential between the facilitated and nonfacilitated transfer. The cation chosen, N-methylphenanthroline, is a known DNA intercalator, and analysis of the steady-state wave for facilitated transfer allows an estimate (2.8) of the number of ions transferred per molecule of oligonucleotide arriving at the interface. The DNA−methylphenanthroline complex adsorbs at the interface, and a stripping peak for extraction of N-methylphenanthroline from the adsorbed DNA back into the organic phase is observed on reversing the scan direction.

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Section: Resultssupporting

confidence: 53%

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confidence: 99%

Cation Binding to DNA Studied by Ion-Transfer Voltammetry at Micropipets

Horrocks

Mirkin

1998

Anal. Chem.

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“…Especially from curves 3 and 4 strong differences between ds-DNA and ss-DNA can be observed giving evidence for not the same adsorbed state. From these results and others found in the laboratory Bioelectrochemistry a model has been proposed based on 5 processes [41]: Our model for adsorption and potential induced compaction of DNA pulse polarographic data: (1) The adsorbed segments become partially dehydrated and the ionic double layer becomes disturbed.…”

Section: Influence Of Ionic Strength On the Adsorption Behavior Of Domentioning

confidence: 77%

Selected Contributions to Polarography by the Bioelectrochemistry Laboratory at Jena

Berg

2003

Review of Polarography

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“…[5][6][7][8][9][10] Electroanalytical methods have become important to the investigation of nucleic acids and several reports have appeared concerning the electrochemistry of DNA and RNA and metal complex-nucleic acid interaction. [11][12][13][14][15][16][17] Recently, several workers have described the use of metal complexes for the recognition of double stranded nucleic acids and have demonstrated possible DNA hybridisation sensors based on cyclic voltammetry, 18 electrogenerated chemiluminescence 19 and the quartz crystal microbalance. 19,20 These sensors detect the hybridisation of single stranded DNA with complementary immobilised probe oligomers.…”

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confidence: 99%