Corrigendum: Detection and quantification of airborne inoculum of <i>Sclerotinia sclerotiorum</i> using quantitative PCR

Rogers, S. L.; Atkins, Simon D.; West, Jonathan

doi:10.1111/j.1365-3059.2011.02447.x

Cited by 39 publications

(58 citation statements)

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“…As reported in literature, other primers have been described as successful for the detection of S. sclerotiorum for ascospores and in plant tissues infected by the pathogen (Yanni et al, 2009;Kim and Knudsen, 2008;Rogers et al, 2009). In this work these primers were tested in soybeans seeds inoculated with S. sclerotiorum but without success.…”

Section: Specificity Of the Primersmentioning

confidence: 94%

Detection of Sclerotinia sclerotiorum in soybean seeds by conventional and quantitative PCR techniques

Botelho

Barrocas²,

Machado

et al. 2015

J. Seed Sci.

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-Sclerotinia sclerotiorum, the etiological agent of the "white mould" in soybean, is responsible for severe losses in this crop and soil contamination. The introduction and dissemination of the disease can made through the use of seed lots contaminated with sclerotia and by seeds infected by mycelium. Therefore, seed health quality is one aspect to be monitored by means of health testing before to sowing time. In this study conventional and quantitative PCR techniques were used to assess their viability to detect S. sclerotiorum in artificially and naturally infected soybean seed samples. For that, seeds were inoculated by osmotic conditioning technique for 0, 24, 48 and 72 hours of contact of the seed with the fungal colony and mixed with healthy seeds generating incidence levels of 1, 2, 10, 20 and 100% for each incubation time. The cPCR was sensitive to detect S. sclerotiorum in samples with at least incidence 1% inoculated for 72 hours while the qPCR detected the pathogen in all incidence/inoculum potential combinations. The conventional PCR was able to detect 0.25% of the incidence of S. sclerotiorum in soybean seed lots naturally infected added a preincubation step.Index terms: seed healthy, molecular detection, seed borne pathogen, white mould.Detecção de Sclerotinia sclerotiorum em sementes de soja pelas técnicas de PCR convencional e quantitativo RESUMO -Sclerotinia sclerotiorum causador do "mofo branco" na cultura da soja, é responsável por redução da produção e contaminação do solo. A introdução e disseminação da doença podem se dar pelo uso de sementes contaminadas com escleródios e por sementes infectadas pelo micélio. A qualidade das sementes deve ser monitorada por testes de sanidade antes da semeadura. Neste estudo foram utilizadas técnicas de PCR convencional e quantitativo para avaliar a viabilidade de uso das mesmas para a detecção de S. sclerotiorum em amostras de sementes de soja artificialmente e naturalmente infectadas. Para isso, as sementes foram inoculadas usando a técnica do condicionamento osmótico por 0, 24, 48 e 72 horas de contato das sementes com a colônia do fungo e misturadas à sementes sadias gerando incidência de 1, 2, 10, 20 e 100% para cada tempo de incubação. A técnica de cPCR foi sensível para detectar S. sclerotiorum em amostras com, no mínimo, incidência de 1% inoculadas por 72 horas, enquanto que com o qPCR foi possível detectar o patógeno em todas as combinações incidência/potencial de inóculo. A PCR convencional foi capaz de detectar 0,25% de incidência de S. sclerotiorum em lotes de sementes de soja naturalmente infectados adicionando-se uma etapa de pré-incubação das sementes.Termos para indexação: sanidade de sementes, detecção molecular, patógeno de sementes, mofo branco.

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Section: Specificity Of the Primersmentioning

confidence: 94%

Detection of Sclerotinia sclerotiorum in soybean seeds by conventional and quantitative PCR techniques

Botelho

Barrocas²,

Machado

et al. 2015

J. Seed Sci.

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“…The presence of large numbers of dead spores in those counts may not be ruled out, as they may loose viability during transport because of ambient conditions (Rotem and Aust, 1991). It would be useful for future work to assess the proportion of viable spores in air samples, although determining viable spores with a cultivation method as done in the present study is quite a long and labour-intensive process (at least 2 weeks were needed to ensure that collected spores were B. cinerea) compared to direct microscopic quantification of spores or possible assessment via quantitative PCR (Rogers et al, 2009). …”

Section: Discussionmentioning

confidence: 99%

Monitoring viable airborne inoculum of Botrytis cinerea in the South-East of France over 3 years: relation with climatic parameters and the origin of air masses

Leyronas¹,

Nicot²

2012

Aerobiologia

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Airborne inoculum of Botrytis cinerea was monitored bimonthly during three years (Sept. 2007-Dec. 2010) on a site in the South-East of France located approximately 5 km away from susceptible crops.Viable inoculum was collected for 96% of the sampling days, including during cold winter periods and hot and dry summer conditions. The concentration of airborne inoculum was significantly higher during daytime than at night. Peaks of concentration were recorded at different periods each year (Sept-Oct in 2008, May in 2010. The abundance of viable inoculum was positively correlated with average daily relative humidity and negatively correlated with air temperature and solar radiation. The analysis of backward trajectories suggested that air masses originating from the North or the South brought more viable inoculum than those from the West. This study showed that susceptible crops may be at danger from viable inoculum of B. cinerea during all seasons of the year, but that risk prediction models could be developed on the basis of climatic conditions and the origin of air masses.

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“…However, qPCR has been also used to quantify the airborne inoculum of fungal pathogens, such as Sclerotinia sclerotiorum, Leptosphaeria spp., Puccinia striiformis, and Botrytis squamosa (38,39,40,41), which cause serious disease in arable crops, and also for the forest tree pathogen Fusarium circinatum (20,42). Many fungal diseases are initiated by airborne inoculum that lands on susceptible hosts under favorable environmental conditions.…”

Section: Discussionmentioning

confidence: 99%

Rapid Detection of Ceratocystis platani Inoculum by Quantitative Real-Time PCR Assay

Luchi¹,

Ghelardini²,

Belbahri

et al. 2013

Appl Environ Microbiol

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Ceratocystis platani is the causal agent of canker stain of plane trees, a lethal disease able to kill mature trees in one or two successive growing seasons. The pathogen is a quarantine organism and has a negative impact on anthropogenic and natural populations of plane trees. Contaminated sawdust produced during pruning and sanitation fellings can contribute to disease spread. The goal of this study was to design a rapid, real-time quantitative PCR assay to detect a C. platani airborne inoculum. Airborne inoculum traps (AITs) were placed in an urban setting in the city of Florence, Italy, where the disease was present. Primers and TaqMan minor groove binder (MGB) probes were designed to target cerato-platanin (CP) and internal transcribed spacer 2 (ITS2) genes. The detection limits of the assay were 0.05 pg/l and 2 fg/l of fungal DNA for CP and ITS, respectively. Pathogen detection directly from AITs demonstrated specificity and high sensitivity for C. platani, detecting DNA concentrations as low as 1.2 ؋ 10 ؊2 to 1.4 ؋ 10 ؊2 pg/l, corresponding to ϳ10 conidia per ml. Airborne inoculum traps were able to detect the C. platani inoculum within 200 m of the closest symptomatic infected plane tree. The combination of airborne trapping and real-time quantitative PCR assay provides a rapid and sensitive method for the specific detection of a C. platani inoculum. This technique may be used to identify the period of highest risk of pathogen spread in a site, thus helping disease management.

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Corrigendum: Detection and quantification of airborne inoculum of Sclerotinia sclerotiorum using quantitative PCR

Abstract: Corrigendum to: Rogers SL, Atkins SD, West JS, 2009. Detection and quantification of airborne inoculum of Sclerotinia sclerotiorum using quantitative PCR. Plant Pathology 58: 324-331.

Cited by 39 publications

References 0 publications

Detection of Sclerotinia sclerotiorum in soybean seeds by conventional and quantitative PCR techniques

Detection of Sclerotinia sclerotiorum in soybean seeds by conventional and quantitative PCR techniques

Monitoring viable airborne inoculum of Botrytis cinerea in the South-East of France over 3 years: relation with climatic parameters and the origin of air masses

Rapid Detection of Ceratocystis platani Inoculum by Quantitative Real-Time PCR Assay

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