Corrigendum: High performance thin layer chromatography (HPTLC) method development and validation for determination of doxycycline hyclate in capsule and tablet formulations

Kumssa, Lensi; Layloff, Thomas; Hymete, Ariaya; Ashenef, Ayenew

doi:10.1556/1326.2021.11926

AChrom

2022

DOI: 10.1556/1326.2021.11926

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Corrigendum: High performance thin layer chromatography (HPTLC) method development and validation for determination of doxycycline hyclate in capsule and tablet formulations

Lensi Kumssa

Thomas Layloff

Ariaya Hymete

et al.

Abstract: HPTLC was performed on glass plates coated with C18 reverse phase silica gel 60 F 254 and pretreated with 0.27 M ethylenediaminetetraaceticacid (EDTA) solution. should read as HPTLC was performed on glass plates coated with silica gel 60 F 254 and pretreated with 0.27 M ethylenediaminetetraaceticacid (EDTA) solution.

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Neutral red dye as a novel reagent in spectrophotometric determination of Doxycycline via oxidation and bleaching color of the dye

Khalaf

Othman

2023

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A simple and accrued spectrophotometric method was developed to estimate Doxycycline as pure and in its formulation. The method included using a novel reagent neutral red dye the present method. The method was based on the oxidation of Doxycycline with an excess amount of N-bromosucinimide in an acid medium, then the residual amount of N-bromosucinimide oxidized the neutral red dye and caused bleaching of the color of the dye. The absorbance of the dye's color did not bleach measured at wavelength 525 nm. The absorbance is proportional to the concentration of Doxycycline in the solution. The linearity was from 1 to 15 µg/ ml, and the higher concentration above 15 µg/ ml gave deviation from Beer's law. The important analytical parameter included molar absorptivity and Sandell's sensitivity index, which were calculated and equal to 3.67 x104 l/mol.cm, 0.01261µg/cm2, respectively. An application part included estimating Doxycycline in its dosage form (capsule) from different manufacturers with satisfactory results. Keywords: Doxycycline, Neutral red, Bleaching Color, Oxidation,

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Neutral red dye as a novel reagent in spectrophotometric determination of Doxycycline via oxidation and bleaching color of the dye

Khalaf

Othman

2023

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Corrigendum: High performance thin layer chromatography (HPTLC) method development and validation for determination of doxycycline hyclate in capsule and tablet formulations

Cited by 1 publication

References 0 publications

Neutral red dye as a novel reagent in spectrophotometric determination of Doxycycline via oxidation and bleaching color of the dye

Neutral red dye as a novel reagent in spectrophotometric determination of Doxycycline via oxidation and bleaching color of the dye

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