Corrigendum: RNA-DNA hybrid (R-loop) immunoprecipitation mapping: an analytical workflow to evaluate inherent biases

Abstract

The authors would like to correct erroneous text relating to the Step-by-step protocol of the best-performing DRIP experiment (exp. 5) section in the Supplemental Material. The corrected text is as follows and has been updated in the Revised Supplemental Material online: "Cells were lysed in the lysis buffer provided by the NucleoSpin Tissue kit (Macherey-Nagel) at 65°C for 7 h (according to the kit protocol), or at 37°C overnight (where indicated in the main text)."

Resolution of R-loops by INO80 promotes DNA replication and maintains cancer cell proliferation and viability

“…Recombinant RNAse H (NEB #M0297) was used following standard protocol 66 with the modification that fixed cells on coverslips were digested with RNase H in RNAse H buffer for 2 h at 37 °C, after which coverslips were washed 3 ×10′ in PBS prior to immunofluorescence staining. Control samples were mock-treated with RNase H buffer.…”

“…DRIP assay was performed by performing IP with the S9.6 antibody in 2.5 μg genomic DNA and following the protocol of experiment 5 in ref. 66 . The primers used in the qPCR assay are as follows:…”