“…Extraction was performed using the system described in Thakore et al 18 and the protocol described in Thakore et al 19 with the exception of an additional offline heating step of 56 o C for 10 minutes (in the case of the clinical studies); the system at LME did not include an onboard heater, but the system at Akonni did. The real-time PCR assay, described in Thakore et al 19 , was used in these studies.…”
Section: Extraction Using Sample Prep Systemmentioning
confidence: 99%
“…We previously reported an automated extraction method for isolating MTB DNA from sputum 18,19 . We also reported an integrated microfluidic amplification microarray that included a gel element array printed on a glass substrate.…”
“…Extraction was performed using the system described in Thakore et al 18 and the protocol described in Thakore et al 19 with the exception of an additional offline heating step of 56 o C for 10 minutes (in the case of the clinical studies); the system at LME did not include an onboard heater, but the system at Akonni did. The real-time PCR assay, described in Thakore et al 19 , was used in these studies.…”
Section: Extraction Using Sample Prep Systemmentioning
confidence: 99%
“…We previously reported an automated extraction method for isolating MTB DNA from sputum 18,19 . We also reported an integrated microfluidic amplification microarray that included a gel element array printed on a glass substrate.…”
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