Corrigendum to “A portable low-cost long-term live-cell imaging platform for biomedical research and education” [Biosens. Bioelectron. 64 (2015) 639–649]

Walzik, Maria P.; Vollmar, Verena; Lachnit, Theresa; Dietz, Helmut; Haug, Susanne; Bachmann, Holger; Fath, Moritz; Aschenbrenner, Daniel; Mofrad, Sepideh Abolpour; Friedrich, Oliver; Gilbert, Daniel F.

doi:10.1016/j.bios.2017.08.021

Cited by 2 publications

(3 citation statements)

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“…[9][10][11] Currently, smart phone-based biosensors have been used in light microscopy, 12 fluorescence imaging, [13][14][15] fluorescence cytometry, 16 colorimetric biosensors, 17,18 surface plasmon resonance-based sensing, 19 electrochemical sensing, 20,21 lateral flow devices, [22][23][24] microfluidic chips, [25][26][27] genetic analysis, 28 and other applications. 22,[29][30][31][32][33] For example, a series of smart phone-based biosensors have been reported for liquid assay readouts. 31,34 Vashist reported a smart phone-based colorimetric reader that uses the back camera of a smart phone to photograph the liquid assay product and then uses software (Image J 1.48v) to calculate the pixel intensity of the image.…”

Section: Introductionmentioning

confidence: 99%

A portable smart phone-based plasmonic nanosensor readout platform that measures transmitted light intensities of nanosubstrates using an ambient light sensor

et al. 2016

Lab Chip

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Plasmonic nanosensors may be used as tools for diagnostic testing in the field of medicine. However, quantification of plasmonic nanosensors often requires complex and bulky readout instruments. Here, we report the development of a portable smart phone-based plasmonic nanosensor readout platform (PNRP) for accurate quantification of plasmonic nanosensors. This device operates by transmitting excitation light from a LED through a nanosubstrate and measuring the intensity of the transmitted light using the ambient light sensor of a smart phone. The device is a cylinder with a diameter of 14 mm, a length of 38 mm, and a gross weight of 3.5 g. We demonstrated the utility of this smart phone-based PNRP by measuring two well-established plasmonic nanosensors with this system. In the first experiment, the device measured the morphology changes of triangular silver nanoprisms (AgNPRs) in an immunoassay for the detection of carcinoembryonic antigen (CEA). In the second experiment, the device measured the aggregation of gold nanoparticles (AuNPs) in an aptamer-based assay for the detection of adenosine triphosphate (ATP). The results from the smart phone-based PNRP were consistent with those from commercial spectrophotometers, demonstrating that the smart phone-based PNRP enables accurate quantification of plasmonic nanosensors.

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Section: Introductionmentioning

confidence: 99%

A portable smart phone-based plasmonic nanosensor readout platform that measures transmitted light intensities of nanosubstrates using an ambient light sensor

et al. 2016

Lab Chip

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“…Indeed, growing efforts have been made to develop compact imaging systems so that cell functional assays can be performed without requiring specialized microscopy facilities. For example, various incubation microscopes were developed so the microscope can be placed inside a conventional incubator for cell imaging or directly control the temperature of the cell assay within the portable microscope (Jin et al 2015;Pushkarsky et al 2014;Walzik et al 2015;Zhang et al 2015). A highly integrated portable and robotically controlled live cell imaging system was employed for cell migration assay in a microfluidic device (Saito et al 2016).…”

Section: Introductionmentioning

confidence: 99%

“…A highly integrated portable and robotically controlled live cell imaging system was employed for cell migration assay in a microfluidic device (Saito et al 2016). In addition, USB microscopes or webcams were also used for functional cell and tissue imaging, which significantly lower the costs while maintaining adequate imaging performance (Isikman et al 2012;Kim et al 2011;Kim et al 2012;Lynch et al 2014;Walzik et al 2015). We have previously performed cell chemotaxis test using a USB microscope based portable system and a smartphone for remote data monitoring (Wu et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Mkit: A cell migration assay based on microfluidic device and smartphone

Yang

Peretz‐Soroka

et al. 2018

Biosensors and Bioelectronics

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Mobile sensing based on the integration of microfluidic device and smartphone, so-called MS 2 technology, has enabled many applications over recent years, and continues to stimulate growing interest in both research communities and industries. In particular, it has been envisioned that MS 2 technology can be developed for various cell functional assays to enable basic research and clinical applications. Toward this direction, in this paper, we describe the development of a MS 2 -based cell functional assay for testing cell migration (the M kit ). The system is constructed as an integrated test kit, which includes microfluidic chips, a smartphone-based imaging platform, the phone apps for image capturing and data analysis, and a set of reagent and accessories for performing the cell migration assay. We demonstrated that the M kit can effectively measure purified neutrophil and cancer cell chemotaxis. Furthermore, neutrophil chemotaxis can be tested from a drop of whole blood using the M kit with red blood cell (RBC) lysis. The effects of chemoattractant dose and gradient profile on neutrophil chemotaxis were also tested using the M kit . In addition to research applications, we demonstrated the effective use of the M kit for on-site test at the hospital and for testing clinical samples from chronic obstructive pulmonary disease patient. Thus, this developed M kit provides an easy and integrated experimental platform for cell migration related research and potential medical diagnostic applications.

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Corrigendum to “A portable low-cost long-term live-cell imaging platform for biomedical research and education” [Biosens. Bioelectron. 64 (2015) 639–649]

Cited by 2 publications

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A portable smart phone-based plasmonic nanosensor readout platform that measures transmitted light intensities of nanosubstrates using an ambient light sensor

A portable smart phone-based plasmonic nanosensor readout platform that measures transmitted light intensities of nanosubstrates using an ambient light sensor

Mkit: A cell migration assay based on microfluidic device and smartphone

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